PMID- 30990360
OWN - NLM
STAT- MEDLINE
DCOM- 20200601
LR  - 20200601
IS  - 1557-7465 (Electronic)
IS  - 1079-9907 (Linking)
VI  - 39
IP  - 6
DP  - 2019 Jun
TI  - Differences in Lipopolysaccharides-Induced Inflammatory Response Between Mouse 
      Embryonic Fibroblasts and Bone Marrow-Derived Macrophages.
PG  - 375-382
LID - 10.1089/jir.2018.0167 [doi]
AB  - Mouse embryonic fibroblasts (MEFs) are commonly used in research on the molecular 
      mechanism(s) of inflammation because of its good response to inflammatory 
      stimuli. However, the difference in inflammatory reaction between MEFs and 
      macrophages, a classical inflammatory cell type, has not been identified. In this 
      study, we report that both mRNA and protein levels of interleukin-6 (IL-6) and 
      tumor necrosis factor-alpha (TNF-alpha) in MEFs upon lipopolysaccharides (LPS) 
      stimulation were significantly lower than those in bone marrow-derived 
      macrophages (BMDMs). MAPK, NF-kappaB, and IRF3 pathways control the expression and 
      production of inflammatory activated in LPS-stimulated MEFs, but showed different 
      activation patterns in comparison with LPS-stimulated BMDMs. Upon LPS 
      stimulation, activation of the MAPK pathway was slow and remarkably weaker in 
      MEFs than that in BMDMs, whereas more pronounced activation of both NF-kappaB and 
      IRF3 pathways was observed in MEFs compared to BMDMs. This difference in the 
      activation of MAPK, NF-kappaB, and IRF3 pathways may result in different production 
      of IL-6 and TNF-alpha between MEFs and BMDMs. We further revealed that substantial 
      differences in more additional inflammatory response-related cytokines exist 
      between LPS-stimulated MEFs and BMDMs. In conclusion, MEFs exhibit good 
      responsiveness to LPS as a target cell for inflammation-related research. 
      However, MEFs cannot replace macrophages because of substantial differences in 
      their inflammatory reactivity.
FAU - Li, Yue
AU  - Li Y
AD  - Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical 
      Sciences, Southern Medical University, Guangzhou, China.
FAU - Niu, Shixian
AU  - Niu S
AD  - Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical 
      Sciences, Southern Medical University, Guangzhou, China.
FAU - Xi, Dalin
AU  - Xi D
AD  - Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical 
      Sciences, Southern Medical University, Guangzhou, China.
FAU - Zhao, Shuqi
AU  - Zhao S
AD  - Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical 
      Sciences, Southern Medical University, Guangzhou, China.
FAU - Sun, Jiang
AU  - Sun J
AD  - Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical 
      Sciences, Southern Medical University, Guangzhou, China.
FAU - Jiang, Yong
AU  - Jiang Y
AD  - Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical 
      Sciences, Southern Medical University, Guangzhou, China.
FAU - Liu, Jinghua
AU  - Liu J
AD  - Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical 
      Sciences, Southern Medical University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190416
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/*drug effects/metabolism
MH  - Inflammation/*chemically induced/metabolism
MH  - Interleukin-6/analysis/antagonists & inhibitors/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
MH  - Pregnancy
MH  - RNA, Messenger/antagonists & inhibitors/metabolism
MH  - Tumor Necrosis Factor-alpha/analysis/antagonists & inhibitors/metabolism
OTO - NOTNLM
OT  - bone marrow-derived macrophages
OT  - inflammatory cytokines
OT  - intracellular signal pathways
OT  - lipopolysaccharide
OT  - mouse embryonic fibroblasts
EDAT- 2019/04/17 06:00
MHDA- 2020/06/02 06:00
CRDT- 2019/04/17 06:00
PHST- 2019/04/17 06:00 [pubmed]
PHST- 2020/06/02 06:00 [medline]
PHST- 2019/04/17 06:00 [entrez]
AID - 10.1089/jir.2018.0167 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2019 Jun;39(6):375-382. doi: 10.1089/jir.2018.0167. 
      Epub 2019 Apr 16.