PMID- 30990806
OWN - NLM
STAT- MEDLINE
DCOM- 20191218
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 4
DP  - 2019
TI  - Towards a low CO2 emission building material employing bacterial metabolism 
      (1/2): The bacterial system and prototype production.
PG  - e0212990
LID - 10.1371/journal.pone.0212990 [doi]
LID - e0212990
AB  - The production of concrete for construction purposes is a major source of 
      anthropogenic CO2 emissions. One promising avenue towards a more sustainable 
      construction industry is to make use of naturally occurring mineral-microbe 
      interactions, such as microbial-induced carbonate precipitation (MICP), to 
      produce solid materials. In this paper, we present a new process where calcium 
      carbonate in the form of powdered limestone is transformed to a binder material 
      (termed BioZEment) through microbial dissolution and recrystallization. For the 
      dissolution step, a suitable bacterial strain, closely related to Bacillus 
      pumilus, was isolated from soil near a limestone quarry. We show that this strain 
      produces organic acids from glucose, inducing the dissolution of calcium 
      carbonate in an aqueous slurry of powdered limestone. In the second step, the 
      dissolved limestone solution is used as the calcium source for MICP in sand 
      packed syringe moulds. The amounts of acid produced and calcium carbonate 
      dissolved are shown to depend on the amount of available oxygen as well as the 
      degree of mixing. Precipitation is induced through the pH increase caused by the 
      hydrolysis of urea, mediated by the enzyme urease, which is produced in situ by 
      the bacterium Sporosarcina pasteurii DSM33. The degree of successful 
      consolidation of sand by BioZEment was found to depend on both the amount of urea 
      and the amount of glucose available in the dissolution reaction.
FAU - Royne, Anja
AU  - Royne A
AUID- ORCID: 0000-0002-6868-6251
AD  - Department of Physics, University of Oslo, Oslo, Norway.
FAU - Phua, Yi Jing
AU  - Phua YJ
AD  - Department of Physics, University of Oslo, Oslo, Norway.
FAU - Balzer Le, Simone
AU  - Balzer Le S
AUID- ORCID: 0000-0002-4933-6643
AD  - SINTEF Industry, Department of Biotechnology and Nanomedicine, Trondheim, Norway.
FAU - Eikjeland, Ina Grosas
AU  - Eikjeland IG
AD  - Department of Physics, University of Oslo, Oslo, Norway.
FAU - Josefsen, Kjell Domaas
AU  - Josefsen KD
AD  - SINTEF Industry, Department of Biotechnology and Nanomedicine, Trondheim, Norway.
FAU - Markussen, Sidsel
AU  - Markussen S
AD  - SINTEF Industry, Department of Biotechnology and Nanomedicine, Trondheim, Norway.
FAU - Myhr, Anders
AU  - Myhr A
AD  - Pure Logic AS, Oslo, Norway.
FAU - Throne-Holst, Harald
AU  - Throne-Holst H
AUID- ORCID: 0000-0002-0606-9725
AD  - Consumption Research Norway (SIFO), OsloMet-Oslo Metropolitan University, Oslo, 
      Norway.
FAU - Sikorski, Pawel
AU  - Sikorski P
AUID- ORCID: 0000-0001-9413-1623
AD  - Department of Physics, Norwegian University of Science and Technology, Trondheim, 
      Norway.
FAU - Wentzel, Alexander
AU  - Wentzel A
AD  - SINTEF Industry, Department of Biotechnology and Nanomedicine, Trondheim, Norway.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190416
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Soil)
RN  - 142M471B3J (Carbon Dioxide)
RN  - EC 3.5.1.5 (Urease)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacillus
MH  - Bacteria/chemistry/*metabolism
MH  - Calcium Carbonate/*chemistry
MH  - Carbon Dioxide/chemistry/*toxicity
MH  - *Chemical Precipitation
MH  - Construction Industry
MH  - Construction Materials
MH  - Humans
MH  - Hydrolysis
MH  - Soil/chemistry
MH  - Urease/chemistry
PMC - PMC6467371
COIS- Royne, Wentzel and Sikorski are inventors on the patent application 
      PCT/EP2017/065509 BIO-CATALYTIC CALCIUM CARBONATE CEMENTATION. This does not 
      alter our adherence to PLOS ONE policies on sharing data and materials.
EDAT- 2019/04/17 06:00
MHDA- 2019/12/19 06:00
PMCR- 2019/04/16
CRDT- 2019/04/17 06:00
PHST- 2018/06/21 00:00 [received]
PHST- 2019/02/10 00:00 [accepted]
PHST- 2019/04/17 06:00 [entrez]
PHST- 2019/04/17 06:00 [pubmed]
PHST- 2019/12/19 06:00 [medline]
PHST- 2019/04/16 00:00 [pmc-release]
AID - PONE-D-18-18560 [pii]
AID - 10.1371/journal.pone.0212990 [doi]
PST - epublish
SO  - PLoS One. 2019 Apr 16;14(4):e0212990. doi: 10.1371/journal.pone.0212990. 
      eCollection 2019.