PMID- 30991705
OWN - NLM
STAT- MEDLINE
DCOM- 20190816
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 8
DP  - 2019 Apr 15
TI  - Oxidative Stress-Tolerant Stem Cells from Human Exfoliated Deciduous Teeth 
      Decrease Hydrogen Peroxide-Induced Damage in Organotypic Brain Slice Cultures 
      from Adult Mice.
LID - 10.3390/ijms20081858 [doi]
LID - 1858
AB  - Oxidative stress causes severe tissue injury of the central nervous system in 
      ischemic brain damage (IBD), traumatic brain injury (TBI) and neurodegenerative 
      disorders. In this study, we used hydrogen peroxide (H(2)O(2)) to induce oxidative 
      stress in organotypic brain slice cultures (OBSCs), and investigated the 
      protective effects of oxidative stress-tolerant (OST) stem cells harvested from 
      human exfoliated deciduous teeth (SHED) which were co-cultivated with OBSCs. 
      Using presto blue assay and immunostaining, we demonstrated that both normal SHED 
      and OST-SHED could prevent H(2)O(2)-induced cell death, and increase the numbers of 
      mature neuron and neuronal progenitors in the hippocampus of OBSCs. During 
      co-cultivation, OST-SHED, but not normal SHED, exhibited neuronal cell morphology 
      and expressed neuronal markers. Results from ELISA showed that both normal SHED 
      and OST-SHED significantly decreased oxidative DNA damage in H(2)O(2)-treated OBSCs. 
      SHED could also produce neurotrophic factor BDNF (brain derived neurotrophic 
      factor) and promoted the production of IL-6 in OBSCs. Although OST-SHED had lower 
      cell viability, the neuronal protection of OST-SHED was significantly superior to 
      that of normal SHED. Our findings suggest that SHED, especially OST-SHED, could 
      prevent oxidative stress induced brain damage. OST-SHED can be explored as a new 
      therapeutic tool for IBD, TBI and neurodegenerative disorders.
FAU - Xiao, Li
AU  - Xiao L
AUID- ORCID: 0000-0002-3053-4930
AD  - Department of Pharmacology, School of Life Dentistry at Tokyo, The Nippon Dental 
      University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-0071, Japan. 
      xiaoli@tky.ndu.ac.jp.
FAU - Saiki, Chikako
AU  - Saiki C
AD  - Department of Physiology, School of Life Dentistry at Tokyo, The Nippon Dental 
      University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-0071, Japan. 
      chikako@tky.ndu.ac.jp.
FAU - Okamura, Hisashi
AU  - Okamura H
AD  - Department of Oral and Maxillofacial Surgery, The Nippon Dental University 
      Hospital, 2-3-16 Fujimi, Chiyoda-ku, Tokyo 102-8158, Japan. hisashi124@gmail.com.
LA  - eng
GR  - 17K11813/Japan Society for the Promotion of Science (JSPS)/
PT  - Journal Article
DEP - 20190415
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - BBX060AN9V (Hydrogen Peroxide)
MH  - Animals
MH  - Brain/*cytology
MH  - Cell Death
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Child
MH  - *Coculture Techniques
MH  - Female
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Mice, Inbred ICR
MH  - Neurogenesis
MH  - Neurons/*cytology
MH  - *Neuroprotection
MH  - *Oxidative Stress
MH  - Stem Cells/*cytology
MH  - Tooth, Deciduous/*cytology
PMC - PMC6514841
OTO - NOTNLM
OT  - brain damage
OT  - brain slice culture
OT  - neuronal protection
OT  - oxidative stress-tolerant stem cells
OT  - stem cells from human exfoliated deciduous teeth
COIS- The authors declare no conflict of interest.
EDAT- 2019/04/18 06:00
MHDA- 2019/08/17 06:00
PMCR- 2019/04/01
CRDT- 2019/04/18 06:00
PHST- 2019/03/21 00:00 [received]
PHST- 2019/04/11 00:00 [revised]
PHST- 2019/04/12 00:00 [accepted]
PHST- 2019/04/18 06:00 [entrez]
PHST- 2019/04/18 06:00 [pubmed]
PHST- 2019/08/17 06:00 [medline]
PHST- 2019/04/01 00:00 [pmc-release]
AID - ijms20081858 [pii]
AID - ijms-20-01858 [pii]
AID - 10.3390/ijms20081858 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Apr 15;20(8):1858. doi: 10.3390/ijms20081858.