PMID- 30992382
OWN - NLM
STAT- MEDLINE
DCOM- 20200225
LR  - 20200225
IS  - 1470-8736 (Electronic)
IS  - 0143-5221 (Linking)
VI  - 133
IP  - 9
DP  - 2019 May 15
TI  - CircRASSF2 promotes laryngeal squamous cell carcinoma progression by regulating 
      the miR-302b-3p/IGF-1R axis.
PG  - 1053-1066
LID - 10.1042/CS20190110 [doi]
AB  - Background: Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) 
      broadly expressed in cells of various species. However, the molecular mechanisms 
      that link circRNAs with laryngeal squamous cell carcinoma (LSCC) are not well 
      understood. In the present study, we attempted to provide novel basis for 
      targeted therapy for LSCC from the aspect of circRNA-microRNA (miRNA)-mRNA 
      interaction.Methods: We investigated the expression of circRNAs in three paired 
      LSCC tissues and adjacent non-tumor tissues by microarray analysis. 
      Differentially expressed circRNAs were identified between LSCC tissues and 
      non-cancerous matched tissues, including 527 up-regulated circRNAs and 414 
      down-regulated circRNAs. We focused on hsa_circ_0059354, which is located on 
      chromosome 20 and derived from RASSF2, and thus we named it circRASSF2.Results: 
      circRASSF2 was found to be significantly up-regulated in LSCC tissues and LSCC 
      cell lines compared with paired adjacent non-tumorous tissues and normal cells. 
      Moreover, knockdown of circRASSF2 significantly inhibited cell proliferation and 
      migration in vitro, which was blocked by miR-302b-3p inhibitor. Bioinformatics 
      analysis predicted that there is a circRASSF2/miR-302b-3p/ insulin-like growth 
      factor 1 receptor (IGF-1R) axis in LSCC progression. Dual-luciferase reporter 
      system validated the direct interaction of circRASSF2, miR-302b-3p, and IGF-1R. 
      Western blot verified that inhibition of circRASSF2 decreased IGF-1R expression. 
      Furthermore, silencing circRASSF2 suppressed LSCC growth in vivo Importantly, we 
      demonstrated that circRASSF2 was up-regulated in serum exosomes from LSCC 
      patients. Altogether, silencing circRASSF2 suppresses progression of LSCC by 
      interacting with miR-302b-3p and decreasing inhibiting IGF-1R 
      expression.Conclusion: In conclusion, these data suggest that circRASSF2 is a 
      central component linking circRNAs to progression of LSCC via an 
      miR-302b-3p/IGF-1R axis.
CI  - (c) 2019 The Author(s).
FAU - Tian, Linli
AU  - Tian L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China.
FAU - Cao, Jing
AU  - Cao J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China.
FAU - Jiao, Hui
AU  - Jiao H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China.
FAU - Zhang, Jiarui
AU  - Zhang J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China.
FAU - Ren, Xiuxia
AU  - Ren X
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China.
FAU - Liu, Xinyu
AU  - Liu X
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China.
FAU - Liu, Ming
AU  - Liu M
AUID- ORCID: 0000-0001-6219-4451
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China jianguang1612@163.com 76202920@qq.com.
FAU - Sun, Yanan
AU  - Sun Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, No. 148 Baojian Road, Nangang District, 
      Harbin 150086, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190509
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (IGF1R protein, human)
RN  - 0 (MIRN302A microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RASSF2 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Down-Regulation
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - MicroRNAs/*genetics
MH  - Receptor, IGF Type 1/*genetics
MH  - Tumor Suppressor Proteins/*genetics
MH  - Up-Regulation
OTO - NOTNLM
OT  - IGF-1R
OT  - LSCC
OT  - ceRNAs,
OT  - circRASSF2
OT  - exosome
OT  - miR-302b-3p
EDAT- 2019/04/18 06:00
MHDA- 2020/02/26 06:00
CRDT- 2019/04/18 06:00
PHST- 2019/02/27 00:00 [received]
PHST- 2019/04/08 00:00 [revised]
PHST- 2019/04/15 00:00 [accepted]
PHST- 2019/04/18 06:00 [pubmed]
PHST- 2020/02/26 06:00 [medline]
PHST- 2019/04/18 06:00 [entrez]
AID - CS20190110 [pii]
AID - 10.1042/CS20190110 [doi]
PST - epublish
SO  - Clin Sci (Lond). 2019 May 9;133(9):1053-1066. doi: 10.1042/CS20190110. Print 2019 
      May 15.