PMID- 30994326
OWN - NLM
STAT- MEDLINE
DCOM- 20200908
LR  - 20200908
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 91
IP  - 9
DP  - 2019 May 7
TI  - Design and Demonstration of Tunable Amplified Sensitivity of AlGaN/GaN High 
      Electron Mobility Transistor (HEMT)-Based Biosensors in Human Serum.
PG  - 5953-5960
LID - 10.1021/acs.analchem.9b00353 [doi]
AB  - We have developed a swift and simplistic protein immunoassay using aptamer 
      functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique 
      design of the sensor facilitates protein detection in a physiological salt 
      environment overcoming charge screening effects, without requiring sample 
      preprocessing. This study reports a tunable and amplified sensitivity of 
      solution-gated electric double layer (EDL) HEMT-based biosensors, which 
      demonstrates significantly enhanced sensitivity by designing a smaller gap 
      between the gate electrode and the detection, and by operating at higher gate 
      voltage. Sensitivity is calculated by quantifying NT-proBNP, a clinical biomarker 
      of heart failure, in buffer and untreated human serum samples. The biosensor 
      depicts elevated sensitivity and high selectivity. Furthermore, detailed 
      investigation of the amplified sensitivity in an increased ionic strength 
      environment is conducted, and it is revealed that a high sensitivity of 80.54 
      mV/decade protein concentration can be achieved, which is much higher than that 
      of previously reported FET biosensors. This sensor technology demonstrates 
      immense potential in developing surface affinity sensors for clinical 
      diagnostics.
FAU - Tai, Tse-Yu
AU  - Tai TY
FAU - Sinha, Anirban
AU  - Sinha A
AUID- ORCID: 0000-0002-4520-2859
FAU - Sarangadharan, Indu
AU  - Sarangadharan I
FAU - Pulikkathodi, Anil Kumar
AU  - Pulikkathodi AK
FAU - Wang, Shin-Li
AU  - Wang SL
FAU - Lee, Geng-Yen
AU  - Lee GY
AD  - Department of Electrical Engineering , National Central University , Zhongli 
      District, Taoyuan City 320 , Taiwan, R.O.C.
FAU - Chyi, Jen-Inn
AU  - Chyi JI
AD  - Department of Electrical Engineering , National Central University , Zhongli 
      District, Taoyuan City 320 , Taiwan, R.O.C.
FAU - Shiesh, Shu-Chu
AU  - Shiesh SC
AD  - Department of Medical Laboratory Science and Biotechnology , National Cheng Kung 
      University , Tainan City 701 , Taiwan, R.O.C.
FAU - Lee, Gwo-Bin
AU  - Lee GB
FAU - Wang, Yu-Lin
AU  - Wang YL
AUID- ORCID: 0000-0002-4791-936X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190424
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aluminum Compounds)
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Biomarkers)
RN  - 0 (Peptide Fragments)
RN  - 0 (aluminum gallium nitride)
RN  - 0 (pro-brain natriuretic peptide (1-76))
RN  - 114471-18-0 (Natriuretic Peptide, Brain)
RN  - 1R9CC3P9VL (gallium nitride)
RN  - CH46OC8YV4 (Gallium)
SB  - IM
MH  - Aluminum Compounds/*chemistry
MH  - Aptamers, Nucleotide/chemistry
MH  - Biomarkers/analysis
MH  - Biosensing Techniques/*methods
MH  - *Electrons
MH  - Gallium/*chemistry
MH  - Humans
MH  - Natriuretic Peptide, Brain/*blood/chemistry
MH  - Peptide Fragments/*blood/chemistry
MH  - *Transistors, Electronic
EDAT- 2019/04/18 06:00
MHDA- 2020/09/09 06:00
CRDT- 2019/04/18 06:00
PHST- 2019/04/18 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
PHST- 2019/04/18 06:00 [entrez]
AID - 10.1021/acs.analchem.9b00353 [doi]
PST - ppublish
SO  - Anal Chem. 2019 May 7;91(9):5953-5960. doi: 10.1021/acs.analchem.9b00353. Epub 
      2019 Apr 24.