PMID- 30994959
OWN - NLM
STAT- MEDLINE
DCOM- 20200831
LR  - 20211204
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 8
DP  - 2019 Aug
TI  - Silencing of microRNA-27a facilitates autophagy and apoptosis of melanoma cells 
      through the activation of the SYK-dependent mTOR signaling pathway.
PG  - 13262-13274
LID - 10.1002/jcb.28600 [doi]
AB  - Melanoma is considered as an aggressive neoplastic transformation and featured 
      with high metastatic potential. Although some studies have provided targets for 
      novel therapeutic interventions, clinical development of targeted drugs for 
      melanoma still remains obscure. Therefore, this study aims to identify the role 
      of microRNA-27a (miR-27a) in autophagy and apoptosis of melanoma cells in 
      regulating spleen tyrosine kinase (SYK)-mediated the mammalian target of 
      rapamycin (mTOR) signaling pathway. A microarray-based analysis was made to 
      screen differentially expressed genes and predict target miRNA. Melanoma 
      specimens were collected with pigmented nevus as a control. Melanoma cell line 
      Mel-RM was treated with miR-27a inhibitor or pcDNA-SYK to prove their effects on 
      autophagy and apoptosis of melanoma cells. The volume change and tumor mass of 
      nude mice in each group were detected by the tumorigenesis assay. 
      Microarray-based analysis results showed that SYK was lowly expressed in melanoma 
      cells and may be regulated by miR-27a. Besides, miR-27a expression was increased 
      whereas SYK expression was decreased in melanoma tissues. Meanwhile, miR-27a was 
      positively correlated with tumor stage and lymph node metastasis of melanoma 
      tissues. Furthermore, miR-27a targeted SYK and silencing of miR-27a or 
      overexpression of SYK cells promoted autophagy and apoptosis of melanoma cells 
      and reduced their tumorigenic ability in vivo. In conclusion, this study proves 
      that silencing of miR-27a facilitates autophagy and apoptosis of melanoma cells 
      by upregulating SYK expression and activating the mTOR signaling pathway. The 
      finding offers new ideas for the clinical development of melanoma.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Tang, Hua
AU  - Tang H
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Xu, Xiaopeng
AU  - Xu X
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Xiao, Weirong
AU  - Xiao W
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Liao, Yangying
AU  - Liao Y
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Xiao, Xiao
AU  - Xiao X
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Li, Lan
AU  - Li L
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Li, Ke
AU  - Li K
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Jia, Xiaomin
AU  - Jia X
AD  - Department of Pathology, Hunan Provincial People's Hospital, The First Affiliated 
      Hospital of Hunan Normal University, Changsha, P.R. China.
FAU - Feng, Hao
AU  - Feng H
AUID- ORCID: 0000-0001-9850-0422
AD  - Department of Dermatology, Hunan Provincial People's Hospital, The First 
      Affiliated Hospital of Hunan Normal University, Changsha, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190417
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (MIRN27 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics/physiology
MH  - Autophagy/genetics/*physiology
MH  - Cell Line, Tumor
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Melanoma/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*metabolism
MH  - Signal Transduction/genetics/*physiology
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - apoptosis
OT  - autophagy
OT  - mammalian target of rapamycin (mTOR) signaling pathway
OT  - melanoma
OT  - microRNA-27a (miR-27a)
OT  - spleen tyrosine kinase (SYK)
EDAT- 2019/04/18 06:00
MHDA- 2020/09/01 06:00
CRDT- 2019/04/18 06:00
PHST- 2018/12/28 00:00 [received]
PHST- 2019/02/11 00:00 [accepted]
PHST- 2019/04/18 06:00 [pubmed]
PHST- 2020/09/01 06:00 [medline]
PHST- 2019/04/18 06:00 [entrez]
AID - 10.1002/jcb.28600 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Aug;120(8):13262-13274. doi: 10.1002/jcb.28600. Epub 2019 
      Apr 17.