PMID- 30995924
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20200225
IS  - 1742-2094 (Electronic)
IS  - 1742-2094 (Linking)
VI  - 16
IP  - 1
DP  - 2019 Apr 17
TI  - HLA and killer cell immunoglobulin-like receptor (KIRs) genotyping in patients 
      with acute ischemic stroke.
PG  - 88
LID - 10.1186/s12974-019-1469-5 [doi]
LID - 88
AB  - INTRODUCTION: In humans, a major component of natural killer (NK) and T cell 
      target recognition depends on the surveillance of human leukocyte antigen (HLA) 
      class I molecules by killer immunoglobulin-like receptors (KIRs). AIMS: To 
      implement the knowledge about the immunological genetic background of acute 
      ischemic stroke susceptibility in relation to the frequency of the KIR genes and 
      HLA alleles. METHODS: Subjects with acute ischemic stroke and subjects without 
      stroke were genotyped for the presence of KIR genes and of the three major KIR 
      ligand groups, HLA-C1, HLA-C2, and HLA-Bw4, both HLA-B and HLA-A loci. RESULTS: 
      Between November 2013 and February 2016, consecutive patients with acute ischemic 
      stroke were recruited. As healthy controls, we enrolled subjects without acute 
      ischemic stroke. Subjects with acute ischemic stroke in comparison with controls 
      showed a higher frequency of 2DL3, 2DL5B, 2DS2, and 2DS4 KIR genes and a lower 
      frequency of HLA-B-Bw4(I) alleles. Subjects without acute ischemic stroke showed 
      a higher frequency of interaction between KIR 2DS2 and HLAC2. We also observed a 
      higher frequency of 2DL3 and 2 DL4 KIR genes in subjects with atherosclerotic 
      (LAAS) subtype. Multiple logistic regression analysis showed a protective effect 
      towards stroke of HLA-B-Bw4(I) and interaction between KIR 2DL2 and HLAC1 and 
      2DS2-HLAC2 and a detrimental effect of 2DL2-HLA-C1_A interactions. CONCLUSION: 
      Our findings of a higher frequency of activating KIR genes seem to be consistent 
      with findings previously reported patients with coronary syndrome. This higher 
      frequency of "proinflammatory" genes in subjects with ischemic stroke could also 
      explain the immunoinflammatory activation of the acute phase of stroke.
FAU - Tuttolomondo, Antonino
AU  - Tuttolomondo A
AUID- ORCID: 0000-0001-6440-7318
AD  - Internal Medicine and Stroke Care Ward, Department of Health Promotion Sciences, 
      Maternal and Infant Care, Internal Medicine and Medical Specialties (PROMISE), 
      University of Palermo, P.zza delle Cliniche n.2, 90127, Palermo, Italy. 
      bruno.tuttolomondo@unipa.it.
FAU - Di Raimondo, Domenico
AU  - Di Raimondo D
AD  - Internal Medicine and Stroke Care Ward, Department of Health Promotion Sciences, 
      Maternal and Infant Care, Internal Medicine and Medical Specialties (PROMISE), 
      University of Palermo, P.zza delle Cliniche n.2, 90127, Palermo, Italy.
FAU - Pecoraro, Rosaria
AU  - Pecoraro R
AD  - Pronto Soccorso Unit, Giuseppe Giglio Hospital, Cefalu, Italy.
AD  - PhD Programme in Clinical Medicine and Behavioural Sciences, University of 
      Palermo, Palermo, PA, 90133, Italy.
FAU - Casuccio, Alessandra
AU  - Casuccio A
AD  - Department of Health Promotion Sciences, Maternal and Infant Care, Internal 
      Medicine and Medical Specialties (PROMISE), University of Palermo, P.zza delle 
      Cliniche n.2, 90127, Palermo, Italy.
FAU - Di Bona, Danilo
AU  - Di Bona D
AD  - School and Chair of Allergology, Dipartimento delle Emergenze e Trapianti 
      d'Organo, University of Bari, Bari, Italy.
FAU - Aiello, Anna
AU  - Aiello A
AD  - Dipartimento di Biopatologia e Biotecnologie Mediche, Universita' degli Studi di 
      Palermo, Palermo, Italy.
FAU - Accardi, Giulia
AU  - Accardi G
AD  - Dipartimento di Biopatologia e Biotecnologie Mediche, Universita' degli Studi di 
      Palermo, Palermo, Italy.
FAU - Arnao, Valentina
AU  - Arnao V
AD  - Dipartimento di BioMedicina Sperimentale e Neuroscienze Cliniche, Universita 
      degli Studi di Palermo, Palermo, Italy.
FAU - Clemente, Giuseppe
AU  - Clemente G
AD  - Internal Medicine and Stroke Care Ward, Department of Health Promotion Sciences, 
      Maternal and Infant Care, Internal Medicine and Medical Specialties (PROMISE), 
      University of Palermo, P.zza delle Cliniche n.2, 90127, Palermo, Italy.
FAU - Corte, Vittoriano Della
AU  - Corte VD
AD  - PhD Programme in Molecular and Clinical Medicine, University of Palermo, Palermo, 
      PA, 90133, Italy.
FAU - Maida, Carlo
AU  - Maida C
AD  - Internal Medicine and Stroke Care Ward, Department of Health Promotion Sciences, 
      Maternal and Infant Care, Internal Medicine and Medical Specialties (PROMISE), 
      University of Palermo, P.zza delle Cliniche n.2, 90127, Palermo, Italy.
FAU - Simonetta, Irene
AU  - Simonetta I
AD  - Internal Medicine and Stroke Care Ward, Department of Health Promotion Sciences, 
      Maternal and Infant Care, Internal Medicine and Medical Specialties (PROMISE), 
      University of Palermo, P.zza delle Cliniche n.2, 90127, Palermo, Italy.
FAU - Caruso, Calogero
AU  - Caruso C
AD  - Dipartimento di Biopatologia e Biotecnologie Mediche, Universita' degli Studi di 
      Palermo, Palermo, Italy.
FAU - Squatrito, Rosario
AU  - Squatrito R
AD  - Pronto Soccorso Unit, Giuseppe Giglio Hospital, Cefalu, Italy.
FAU - Pinto, Antonio
AU  - Pinto A
AD  - Internal Medicine and Stroke Care Ward, Department of Health Promotion Sciences, 
      Maternal and Infant Care, Internal Medicine and Medical Specialties (PROMISE), 
      University of Palermo, P.zza delle Cliniche n.2, 90127, Palermo, Italy.
CN  - KIRIIND (KIR Infectious and Inflammatory Diseases) Collaborative Group
LA  - eng
PT  - Journal Article
DEP - 20190417
PL  - England
TA  - J Neuroinflammation
JT  - Journal of neuroinflammation
JID - 101222974
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Receptors, KIR)
SB  - IM
MH  - Aged
MH  - Cross-Sectional Studies
MH  - Female
MH  - Genetic Predisposition to Disease/genetics
MH  - Genotype
MH  - Histocompatibility Antigens Class I/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Receptors, KIR/*genetics
MH  - Stroke/*genetics/*immunology
PMC - PMC6471781
OTO - NOTNLM
OT  - HLA
OT  - Killer immunoglobulin-like receptors (KIRs)
OT  - Stroke
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: This protocol study was approved by 
      the Ethics Committee of the Policlinico P. Giaccone Hospital and of Giuseppe 
      Giglia Hospital, Cefalu (Palermo). All patients gave their written informed 
      consent to participate in the study, as well as for sampling and banking of the 
      biological material. CONSENT FOR PUBLICATION: Not applicable. COMPETING 
      INTERESTS: The authors declare that they have no competing interests. PUBLISHER'S 
      NOTE: Springer Nature remains neutral with regard to jurisdictional claims in 
      published maps and institutional affiliations.
FIR - Tuttolomondo, Antonino
IR  - Tuttolomondo A
FIR - Di Raimondo, Domenico
IR  - Di Raimondo D
FIR - Di Bona, Danilo
IR  - Di Bona D
FIR - Caruso, Calogero
IR  - Caruso C
FIR - Pinto, Antonio
IR  - Pinto A
EDAT- 2019/04/19 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/04/17
CRDT- 2019/04/19 06:00
PHST- 2018/11/25 00:00 [received]
PHST- 2019/03/27 00:00 [accepted]
PHST- 2019/04/19 06:00 [entrez]
PHST- 2019/04/19 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/04/17 00:00 [pmc-release]
AID - 10.1186/s12974-019-1469-5 [pii]
AID - 1469 [pii]
AID - 10.1186/s12974-019-1469-5 [doi]
PST - epublish
SO  - J Neuroinflammation. 2019 Apr 17;16(1):88. doi: 10.1186/s12974-019-1469-5.