PMID- 31002124
OWN - NLM
STAT- MEDLINE
DCOM- 20200813
LR  - 20210308
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 7
DP  - 2019 Apr
TI  - Long noncoding RNA AFAP1-AS1 promoted osteosarcoma proliferation and invasion via 
      upregulating BDNF.
PG  - 2744-2749
LID - 17547 [pii]
LID - 10.26355/eurrev_201904_17547 [doi]
AB  - OBJECTIVE: Recently, long noncoding RNAs (lncRNAs) have got much attention for 
      their role in tumor progression. LncRNA AFAP1-AS1 was studied in this research to 
      identify how it affects the development and proliferation of osteosarcoma. 
      PATIENTS AND METHODS: The mRNA expression of AFAP1-AS1 in osteosarcoma cells and 
      tissue samples was detected by quantitative real-time polymerase chain reaction 
      (qRT-PCR). Moreover, cell proliferation assay and Matrigel assay were performed. 
      Furthermore, the underlying mechanism was explored by using qRT-PCR and Western 
      blot assay. RESULTS: The expression level of AFAP1-AS1 was higher in osteosarcoma 
      samples than that in adjacent tissues. In addition, the proliferation and 
      invasion were inhibited after AFAP1-AS1 was downregulated in vitro. Besides, the 
      mRNA and protein expression levels of brain derived neurotrophic factor (BDNF) 
      were reduced after downregulation of AFAP1-AS1. Furthermore, the expression level 
      of BDNF was positively related to the expression of AFAP1-AS1 in osteosarcoma 
      tissues. CONCLUSIONS: AFAP1-AS1 could enhance the proliferation and invasion of 
      osteosarcoma cells by upregulating BDNF, which might be a potential therapeutic 
      target in osteosarcoma.
FAU - Zhao, B-M
AU  - Zhao BM
AD  - Department of Orthopaedic Surgery, The No. 1 People's Hospital of Jingzhou, 
      Jingzhou, China. cailin225@126.com.
FAU - Cheng, F-H
AU  - Cheng FH
FAU - Cai, L
AU  - Cai L
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (AFAP1-AS1 long noncoding RNA, human)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Long Noncoding)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
RIN - Eur Rev Med Pharmacol Sci. 2020 Sep;24(17):8624. PMID: 32964945
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Disease Progression
MH  - Down-Regulation
MH  - Humans
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - Osteosarcoma/*genetics/pathology
MH  - RNA, Long Noncoding/*genetics
MH  - Up-Regulation
EDAT- 2019/04/20 06:00
MHDA- 2020/08/14 06:00
CRDT- 2019/04/20 06:00
PHST- 2019/04/20 06:00 [entrez]
PHST- 2019/04/20 06:00 [pubmed]
PHST- 2020/08/14 06:00 [medline]
AID - 17547 [pii]
AID - 10.26355/eurrev_201904_17547 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2744-2749. doi: 
      10.26355/eurrev_201904_17547.