PMID- 31002144
OWN - NLM
STAT- MEDLINE
DCOM- 20200908
LR  - 20200908
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 7
DP  - 2019 Apr
TI  - Influences of hucMSC-exosomes on VEGF and BMP-2 expression in SNFH rats.
PG  - 2935-2943
LID - 17573 [pii]
LID - 10.26355/eurrev_201904_17573 [doi]
AB  - OBJECTIVE: To investigate the influences of human umbilical cord mesenchymal stem 
      cell-derived exosomes (hucMSC-exosome) on steroid-induced necrosis of the femoral 
      head (SNFH) and the expressions of vascular endothelial growth factor (VEGF) and 
      bone morphogenetic protein-2 (BMP-2) in rats. PATIENTS AND METHODS: A total of 20 
      male Sprague-Dawley rats were randomly divided into SNFH group and SNFH + 
      hucMSC-exosome group using a random number table. Prednisolone acetate (24.5 
      mg/kg) was injected twice a week to establish the rat model of SNFH, and 
      hucMSC-exosome in a certain dose was additionally injected into the marrow cavity 
      in SNFH + hucMSC-exosome group. After 3 weeks, the influences of hucMSC-exosome 
      on the pathological changes and apoptosis of the femoral head in SNFH rats were 
      detected via hematoxylin-eosin (H&E) staining and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. In addition, the 
      expressions of cluster of differentiation 31 (CD31), VEGF, and BMP-2 in bone 
      tissues in both groups were detected via immunohistochemical staining, and the 
      messenger ribonucleic acid (mRNA) and protein expression levels of VEGF and BMP-2 
      in necrotic bone tissues in both groups were detected via Reverse 
      Transcription-Polymerase Chain Reaction (RT-PCR) and Western blotting. RESULTS: 
      The results of H&E staining revealed that the fibrous callus formation was good, 
      the new trabecular structure was more obvious, the number of vacuum cleft 
      declined, and there were fewer enlarged adipocytes in SNFH + hucMSC-exosome group 
      compared with SNFH group. The results of TUNEL staining showed that the number of 
      apoptotic cells in femoral head tissues was smaller in SNFH + hucMSC-exosome 
      group (p<0.05). According to the results of immunohistochemistry, hucMSC-exosome 
      could increase the expression of vascular endothelial marker CD31 in SNFH rats 
      (p<0.05). Besides, the results of RT-PCR, immunostaining and Western blotting 
      manifested that both the mRNA and protein levels of BMP-2 and VEGF in femoral 
      head tissues were significantly increased in SNFH + hucMSC-exosome group 
      (p<0.05). CONCLUSIONS: HucMSC-exosome can improve SNFH in rats, whose mechanism 
      may be related to the up-regulation of VEGF and BMP-2 by exosomes.
FAU - Li, R
AU  - Li R
AD  - Department of Orthopaedics, The General Hospital of Jinan Military, Jinan, China. 
      1940783143@qq.com.
FAU - Chen, C
AU  - Chen C
FAU - Zheng, R-Q
AU  - Zheng RQ
FAU - Zou, L
AU  - Zou L
FAU - Hao, G-L
AU  - Hao GL
FAU - Zhang, G-C
AU  - Zhang GC
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Protein 2/*biosynthesis/genetics
MH  - Exosomes/*metabolism
MH  - Femur Head Necrosis/genetics/*metabolism/pathology
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Umbilical Cord/cytology/*metabolism
MH  - Vascular Endothelial Growth Factor A/*biosynthesis/genetics
EDAT- 2019/04/20 06:00
MHDA- 2020/09/09 06:00
CRDT- 2019/04/20 06:00
PHST- 2019/04/20 06:00 [entrez]
PHST- 2019/04/20 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
AID - 17573 [pii]
AID - 10.26355/eurrev_201904_17573 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2935-2943. doi: 
      10.26355/eurrev_201904_17573.