PMID- 31002370
OWN - NLM
STAT- MEDLINE
DCOM- 20190820
LR  - 20190820
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 41
IP  - 6
DP  - 2019 Jun
TI  - Long non‑coding RNA RP1‑163G9.1 is downregulated in gastric adenocarcinoma and is 
      associated with a poor prognosis.
PG  - 3575-3585
LID - 10.3892/or.2019.7127 [doi]
AB  - The aim of the present study was to investigate the expression, function and 
      underlying molecular mechanism of the long non‑coding (lnc) RNA RP1‑163G9.1 in 
      patients with gastric adenocarcinoma (GA). The expression levels of lncRNA 
      RP1‑163G9.1 were determined in 112 paired clinical GA tissues by reverse 
      transcription‑quantitative polymerase chain reaction analysis. Subsequently, the 
      potential clinical values of lncRNA RP1‑163G9.1 were analyzed with statistical 
      methods. Additionally, the function of lncRNA RP1‑163G9.1 was explored at the 
      cellular level using the Cell Counting Kit‑8 proliferation assay, Transwell 
      experiments, fluorescence in situ hybridization (FISH), colony formation assay 
      and flow cytometry. Furthermore, the function of lncRNA RP1‑163G9.1 was assessed 
      in vivo using subcutaneous tumorigenesis experiments in nude mice. lncRNA 
      RP1‑163G9.1 expression in GA tissues and cells was significantly decreased when 
      compared with that in control gastric tissues (P<0.001) or gastric epithelial 
      cells GES‑1 (P<0.05). This finding was associated with the depth of invasion 
      (P=0.001), lymph node metastasis (P=0.009), tumor size (P=0.037) and 
      immunocytochemistry marker Ki‑67 (P=0.010). FISH detection demonstrated that 
      lncRNA RP1‑163G9.1 was primarily located in the cytoplasm. Notably, 
      overexpression of lncRNA RP1‑163G9.1 significantly decreased cell proliferation 
      (P<0.01), colony formation (P<0.01), invasion (P<0.01) and the number of cells at 
      the S‑phase of the cell cycle (P<0.05); However, it did not exert a significant 
      effect on apoptosis (P>0.05). Furthermore, tumor formation experiments revealed 
      that overexpression of lncRNA RP1‑163G9.1 inhibited cancer cell proliferation in 
      nude mice. The present research indicated that low expression of lncRNA 
      RP1‑163G9.1 may be associated with enhanced tumor proliferation and invasion in 
      GA.
FAU - Sun, Baochang
AU  - Sun B
AD  - The School of Basic Medical Sciences, Fujian Medical University, Minhou, Fuzhou, 
      Fujian 350108, P.R. China.
FAU - Dang, Yuan
AU  - Dang Y
AD  - Department of Experimental Medicine, Fuzhou General Hospital, Fuzhou, Fujian 
      350025, P.R. China.
FAU - Zhang, Fan
AU  - Zhang F
AD  - The School of Basic Medical Sciences, Fujian Medical University, Minhou, Fuzhou, 
      Fujian 350108, P.R. China.
FAU - Li, Kewen
AU  - Li K
AD  - The School of Basic Medical Sciences, Fujian Medical University, Minhou, Fuzhou, 
      Fujian 350108, P.R. China.
FAU - Ouyang, Xiaojuan
AU  - Ouyang X
AD  - The School of Basic Medical Sciences, Fujian Medical University, Minhou, Fuzhou, 
      Fujian 350108, P.R. China.
FAU - Wang, Kai
AU  - Wang K
AD  - The School of Basic Medical Sciences, Fujian Medical University, Minhou, Fuzhou, 
      Fujian 350108, P.R. China.
FAU - Huang, Qiaojia
AU  - Huang Q
AD  - The School of Basic Medical Sciences, Fujian Medical University, Minhou, Fuzhou, 
      Fujian 350108, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190417
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (RNA, Long Noncoding)
MH  - Adenocarcinoma/*genetics/pathology
MH  - Aged
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Disease-Free Survival
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Prognosis
MH  - RNA, Long Noncoding/*genetics
MH  - Stomach Neoplasms/*genetics/pathology
EDAT- 2019/04/20 06:00
MHDA- 2019/08/21 06:00
CRDT- 2019/04/20 06:00
PHST- 2018/07/29 00:00 [received]
PHST- 2019/04/05 00:00 [accepted]
PHST- 2019/04/20 06:00 [pubmed]
PHST- 2019/08/21 06:00 [medline]
PHST- 2019/04/20 06:00 [entrez]
AID - 10.3892/or.2019.7127 [doi]
PST - ppublish
SO  - Oncol Rep. 2019 Jun;41(6):3575-3585. doi: 10.3892/or.2019.7127. Epub 2019 Apr 17.