PMID- 31004714
OWN - NLM
STAT- MEDLINE
DCOM- 20190610
LR  - 20190613
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 705
DP  - 2019 Jul 15
TI  - BDNF Val66Met genetic variation and its plasma level in patients with morbid 
      obesity: A case-control study.
PG  - 51-54
LID - S0378-1119(19)30398-1 [pii]
LID - 10.1016/j.gene.2019.04.045 [doi]
AB  - Obesity is a major public health concern worldwide. Genetic, behavioral, and 
      environmental factors contribute to the multifactorial etiology of obesity. 
      Evidence suggests an association between human Brain-Derived Neurotrophic Factor 
      (BDNF) Val66Met single nucleotide polymorphism (SNP) and obesity. Reduced plasma 
      BDNF levels have also been reported in patients with eating disorders and 
      obesity. We aimed to evaluate the BDNF Val66Met (rs6265) SNP and also plasma BDNF 
      levels in morbidly obese patients compared with healthy normal controls in 
      southern Iran. One hundred morbidly obese patients and one hundred eight healthy 
      normal controls were enrolled. Blood-derived DNA samples were genotyped using the 
      polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) 
      method and confirmed by DNA sequencing. Plasma BDNF levels were evaluated using a 
      commercially available sandwich enzyme-linked immunosorbent assay (ELISA) kit for 
      human BDNF. Data analysis was performed by SPSS software, version 18.0. Genotype 
      distribution was not significantly different between obese patients and controls. 
      However, plasma BDNF levels were significantly lower in obese patients compared 
      with controls. Interestingly, a significant association was found between BDNF 
      Val66Met SNP and plasma BDNF levels. No relationship was observed between BDNF 
      Val66Met SNP and all assessed demographic and clinical characteristics of obese 
      patients. It seems that plasma BDNF levels were associated with both obesity and 
      BDNF Val66Met SNP. However, this association was not found between BDNF Val66Met 
      SNP and obesity. Further studies with larger sample sizes are needed for more 
      detailed assessment of this genetic variation as a potential biomarker for 
      obesity.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Zamani, Mozhdeh
AU  - Zamani M
AD  - Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Hosseini, Seyed Vahid
AU  - Hosseini SV
AD  - Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Behrouj, Hamid
AU  - Behrouj H
AD  - Department of Biochemistry, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Erfani, Mehran
AU  - Erfani M
AD  - Department of Biochemistry, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Dastghaib, Sanaz
AU  - Dastghaib S
AD  - Department of Biochemistry, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Ahmadi, Mojtaba
AU  - Ahmadi M
AD  - Department of Biochemistry, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran.
FAU - Shamsdin, Seyedeh Azra
AU  - Shamsdin SA
AD  - Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, 
      Shiraz, Iran.
FAU - Mokarram, Pooneh
AU  - Mokarram P
AD  - Department of Biochemistry, School of Medicine, Shiraz University of Medical 
      Sciences, Shiraz, Iran. Electronic address: mokaram2@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20190417
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - AE28F7PNPL (Methionine)
RN  - HG18B9YRS7 (Valine)
SB  - IM
MH  - Adult
MH  - *Amino Acid Substitution
MH  - Brain-Derived Neurotrophic Factor/*blood/*genetics
MH  - Case-Control Studies
MH  - Down-Regulation
MH  - Female
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Iran
MH  - Male
MH  - Methionine/*genetics
MH  - Middle Aged
MH  - Obesity, Morbid/blood/*genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - Sequence Analysis, DNA
MH  - Valine/*genetics
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Obesity
OT  - Polymorphism
EDAT- 2019/04/21 06:00
MHDA- 2019/06/14 06:00
CRDT- 2019/04/21 06:00
PHST- 2018/11/20 00:00 [received]
PHST- 2019/03/23 00:00 [revised]
PHST- 2019/04/16 00:00 [accepted]
PHST- 2019/04/21 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2019/04/21 06:00 [entrez]
AID - S0378-1119(19)30398-1 [pii]
AID - 10.1016/j.gene.2019.04.045 [doi]
PST - ppublish
SO  - Gene. 2019 Jul 15;705:51-54. doi: 10.1016/j.gene.2019.04.045. Epub 2019 Apr 17.