PMID- 31004884
OWN - NLM
STAT- MEDLINE
DCOM- 20190828
LR  - 20190828
IS  - 1618-095X (Electronic)
IS  - 0944-7113 (Linking)
VI  - 59
DP  - 2019 Jun
TI  - Emodin improves glucose metabolism by targeting microRNA-20b in insulin-resistant 
      skeletal muscle.
PG  - 152758
LID - S0944-7113(18)30575-0 [pii]
LID - 10.1016/j.phymed.2018.11.018 [doi]
AB  - BACKGROUND: Emerging evidence has indicated the therapeutic potential of emodin 
      with its multiple pharmacological effects. PURPOSE: To evaluate role of emodin in 
      regulating insulin resistance (IR) and to elucidate the underlying molecular 
      mechanisms. STUDY DESIGN/METHODS: Fasting blood glucose (FBG) and lipid levels 
      were measured before and after intragastric administration of emodin in type 2 
      diabetes mellitus (T2DM) rats. Glucose consumption was determined in L6 cells to 
      investigate the effect of emodin on glucose metabolism. Expression of miR-20b and 
      SMAD7 was quantified by real-time PCR for mRNAs or western blot analysis for 
      proteins. RESULTS: Emodin ameliorated hyperglycemia and dyslipidemia in T2DM 
      rats, and glucose metabolism in a concentration- and time-dependent manner. 
      MiR-20b was markedly upregulated in the setting of IR and overexpression of 
      miR-20b disrupted glucose metabolism by repressing SMAD7 in L6 cells. Knockdown 
      of this miRNA produced the opposite effects. Emodin abolished the abnormal 
      upregulation of miR-20b and indirectly upregulated SMAD7. CONCLUSION: Emodin 
      improves glucose metabolism to produce anti-IR effects, and downregulation of 
      miR-20b thereby upregulation of SMAD7 is an underlying mechanism for the 
      beneficial effects of emodin.
CI  - Copyright (c) 2018. Published by Elsevier GmbH.
FAU - Xiao, Dan
AU  - Xiao D
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Hu, Yingying
AU  - Hu Y
AD  - Department of Pharmacy, the First Affiliated Hospital of Harbin Medical 
      University, Harbin 150081, China.
FAU - Fu, Yujie
AU  - Fu Y
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Wang, Rui
AU  - Wang R
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Zhang, Haiying
AU  - Zhang H
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Li, Mingqi
AU  - Li M
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Li, Zhange
AU  - Li Z
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Xuan, Lina
AU  - Xuan L
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Li, Xin
AU  - Li X
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China.
FAU - Xu, Chaoqian
AU  - Xu C
AD  - Mudanjiang Medical University, 157000, China.
FAU - Zhang, Yong
AU  - Zhang Y
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China; 
      Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, Harbin, 
      150086, China. Electronic address: hmuzhangyong@hotmail.com.
FAU - Yang, Baofeng
AU  - Yang B
AD  - Department of Pharmacology (State-Province Key Laboratories of 
      Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, 
      Ministry of Education), Harbin Medical University, Harbin 150081, China; 
      Department of Pharmacology and Therapeutics, Melbourne School of Biomedical 
      Sciences, Faculty of Medicine, Dentistry and Health Sciences, the University of 
      Melbourne, Melbourne, 3010, Australia. Electronic address: 
      yangbf@ems.hrbmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20181116
PL  - Germany
TA  - Phytomedicine
JT  - Phytomedicine : international journal of phytotherapy and phytopharmacology
JID - 9438794
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (RNA, Messenger)
RN  - 0 (Smad7 Protein)
RN  - 0 (Smad7 protein, rat)
RN  - IY9XDZ35W2 (Glucose)
RN  - KA46RNI6HN (Emodin)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Type 2/drug therapy/*metabolism
MH  - Down-Regulation/drug effects
MH  - Emodin/*pharmacology/therapeutic use
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 4/metabolism
MH  - Hyperglycemia/metabolism/prevention & control
MH  - Insulin/*metabolism
MH  - *Insulin Resistance
MH  - Male
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - *RNA, Messenger/metabolism
MH  - Rats
MH  - Smad7 Protein/metabolism
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - Emodin
OT  - Insulin resistance
OT  - SMAD7
OT  - Skeletal muscle
OT  - miR-20b
EDAT- 2019/04/21 06:00
MHDA- 2019/08/29 06:00
CRDT- 2019/04/21 06:00
PHST- 2018/08/28 00:00 [received]
PHST- 2018/10/27 00:00 [revised]
PHST- 2018/11/15 00:00 [accepted]
PHST- 2019/04/21 06:00 [pubmed]
PHST- 2019/08/29 06:00 [medline]
PHST- 2019/04/21 06:00 [entrez]
AID - S0944-7113(18)30575-0 [pii]
AID - 10.1016/j.phymed.2018.11.018 [doi]
PST - ppublish
SO  - Phytomedicine. 2019 Jun;59:152758. doi: 10.1016/j.phymed.2018.11.018. Epub 2018 
      Nov 16.