PMID- 31005156
OWN - NLM
STAT- MEDLINE
DCOM- 20190701
LR  - 20190701
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1065
DP  - 2019 Aug 13
TI  - Palindromic molecular beacon-based intramolecular strand-displacement 
      amplification strategy for ultrasensitive detection of K-ras gene.
PG  - 98-106
LID - S0003-2670(19)30307-1 [pii]
LID - 10.1016/j.aca.2019.02.059 [doi]
AB  - The sensitive detection of tumor proto-oncogenes is indispensable because the 
      early diagnosis and accurate treatment of genetic diseases is the key guarantee 
      of patients' health. In this study, we proposed a novel palindromic molecular 
      beacon (PMB) that it bases on the signal amplification strategy for 
      ultrasensitive detection of Kras gene codon 12. PMB is designed to have two 
      palindromic fragments at its two ends, one of which is locked via folding into a 
      hairpin structure and the other promotes the formation of PMB duplex via 
      intermolecular self-hybridization. Target DNA can hybridize to the loop portion 
      of PMB and release the palindromic fragment at the 3' end. Within the PMB duplex, 
      the two palindromic fragments released hybridize with each other and serve as 
      polymerization primer responsible for the strand-displacement amplification 
      (SDA). Namely, hybridized target DNA can be displaced and initiates the next 
      round of reactions, making the polymerization/displacement/hybridization process 
      go forward circularly. As a result, a large number of polymerization products are 
      produced, dramatically enhancing optical signal. Because primer hybridization and 
      polymerization-based displacement occur within PMB duplex, the reaction process 
      is called intramolecular strand-displacement amplification (ISDA). Via utilizing 
      the newly-proposed PMB-based ISDA strategy, the target K-ras gene could be 
      detected down to 10 pM with a wide response range of 1 x 10(-11)-1.5 x 10(-7) M, 
      and point mutations are easily distinguished, realizing the ultrasensitive, 
      highly selective detection of K-ras gene. This impressive sensing paradigm 
      demonstrates a new concept of signal amplification for the detection of 
      disease-related genes only via using a simple way to efficiently amplify optical 
      signal.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Li, Hongbo
AU  - Li H
AD  - Key Laboratory of Functional Small Organic Molecule, Ministry of Education, 
      College of Chemistry and Chemical Engineering, Jiangxi Normal University, 
      Nanchang, 330022, PR China. Electronic address: lihongbo112@126.com.
FAU - Tang, Yongqiong
AU  - Tang Y
AD  - Key Laboratory of Functional Small Organic Molecule, Ministry of Education, 
      College of Chemistry and Chemical Engineering, Jiangxi Normal University, 
      Nanchang, 330022, PR China; Cancer Metastasis Alert and Prevention Center, 
      Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy 
      and Environment, Fujian Provincial Key Laboratory of Cancer Metastasis 
      Chemoprevention and Chemotherapy, College of Chemistry, Fuzhou University, 
      Fuzhou, 350002, PR China.
FAU - Zhao, Weihua
AU  - Zhao W
AD  - Key Laboratory of Functional Small Organic Molecule, Ministry of Education, 
      College of Chemistry and Chemical Engineering, Jiangxi Normal University, 
      Nanchang, 330022, PR China.
FAU - Wu, Zaisheng
AU  - Wu Z
AD  - Cancer Metastasis Alert and Prevention Center, Pharmaceutical Photocatalysis of 
      State Key Laboratory of Photocatalysis on Energy and Environment, Fujian 
      Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, 
      College of Chemistry, Fuzhou University, Fuzhou, 350002, PR China. Electronic 
      address: wuzaisheng@163.com.
FAU - Wang, Suqin
AU  - Wang S
AD  - Key Laboratory of Functional Small Organic Molecule, Ministry of Education, 
      College of Chemistry and Chemical Engineering, Jiangxi Normal University, 
      Nanchang, 330022, PR China. Electronic address: wangsuqin163@163.com.
FAU - Yu, Ruqin
AU  - Yu R
AD  - State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry 
      and Chemical Engineering Hunan University, Changsha, 410082, PR China.
LA  - eng
PT  - Journal Article
DEP - 20190319
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
SB  - IM
MH  - *Biosensing Techniques
MH  - Genes, ras/*genetics
MH  - Humans
MH  - *Nucleic Acid Amplification Techniques
MH  - Spectrometry, Fluorescence
OTO - NOTNLM
OT  - Intramolecular strand-displacement amplification (ISDA)
OT  - K-ras gene
OT  - Palindromic molecular beacon (PMB)
OT  - Ultrasensitive detection
EDAT- 2019/04/22 06:00
MHDA- 2019/07/02 06:00
CRDT- 2019/04/22 06:00
PHST- 2018/09/20 00:00 [received]
PHST- 2019/01/11 00:00 [revised]
PHST- 2019/02/27 00:00 [accepted]
PHST- 2019/04/22 06:00 [entrez]
PHST- 2019/04/22 06:00 [pubmed]
PHST- 2019/07/02 06:00 [medline]
AID - S0003-2670(19)30307-1 [pii]
AID - 10.1016/j.aca.2019.02.059 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2019 Aug 13;1065:98-106. doi: 10.1016/j.aca.2019.02.059. Epub 
      2019 Mar 19.