PMID- 31006100
OWN - NLM
STAT- MEDLINE
DCOM- 20191216
LR  - 20200225
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 4
DP  - 2019 Aug
TI  - Yangyin Tongnao granules enhance neurogenesis in the peri-infarct area and 
      upregulate brain-derived neurotrophic factor and vascular endothelial growth 
      factor after focal cerebral ischemic infarction in rats.
PG  - 3817-3826
LID - 10.1007/s11033-019-04824-5 [doi]
AB  - Yangyin Tongnao granules (YYTNG) have been extensively applied in the treatment 
      of brain injury, mainly due to its antioxidant effects, inhibition of apoptosis, 
      and enhancement of blood circulation. To analyze the effect of YYTNG on the 
      recovery of neurological function and neurogenesis in the peri-infarct area after 
      cerebral ischemic infarction in rats and to elucidate its role in the 
      neuroprotective mechanism of stroke, Sprague-Dawley (SD) rats were subjected to 
      middle cerebral artery occlusion (MCAO) for 90 min followed by reperfusion. Rats 
      were randomly divided into five groups: sham, MCAO, and YYTNG-treated rats given 
      doses of 0.83, 1.65, or 3.3 g kg(-1) day(-1). The YYTNG-treated groups (1.65 and 
      3.3 g kg(-1) day(-1)) showed higher neurological scores and a lower infarct 
      volume than the MCAO group on day 3 after MCAO. Furthermore, the YYTNG-treated 
      groups (0.83, 1.65, and 3.3 g kg(-1) day(-1)) showed higher neurological scores 
      on day 7 after MCAO. The number of BrdU(+)/nestin(+), BrdU(+)/NeuN(+), and 
      BrdU(+)/GFAP(+) cells in the peri-infarct area 7 days after MCAO was 
      significantly increased in the YYTNG-treated groups in a dose-dependent manner. 
      The protein expression levels of brain-derived neurotrophic factor (BDNF) and 
      vascular endothelial growth factor (VEGF) were significantly higher in all three 
      YYTNG-treated groups than in the MCAO group. Based on these results, 
      administration of YYTNG post ischemia could ameliorate neurological function 
      deficits in rats with MCAO. The therapeutic effect of YYTNG may be due to the 
      promotion of neurogenesis in the peri-infarct area and the upregulation of 
      neuroprotective factors BDNF and VEGF in MCAO rats.
FAU - Wang, Yu
AU  - Wang Y
AUID- ORCID: 0000-0001-7894-6523
AD  - Zhejiang Chinese Medical University, Hangzhou, 310053, China.
FAU - Yang, Jiehong
AU  - Yang J
AD  - Zhejiang Chinese Medical University, Hangzhou, 310053, China.
FAU - Du, Haixia
AU  - Du H
AD  - Zhejiang Chinese Medical University, Hangzhou, 310053, China.
FAU - Zhang, Hongbo
AU  - Zhang H
AD  - Zhejiang Chinese Medical University, Hangzhou, 310053, China.
FAU - Wan, Haitong
AU  - Wan H
AUID- ORCID: 0000-0001-5726-7214
AD  - Zhejiang Chinese Medical University, Hangzhou, 310053, China. haitongw@163.com.
FAU - He, Yu
AU  - He Y
AD  - Zhejiang Chinese Medical University, Hangzhou, 310053, China. 
      heyu0923@hotmail.com.
LA  - eng
GR  - 81630105/National Natural Science Foundation of China/
GR  - LZ17H270001/Zhejiang Provincial Natural Science Foundation of China/
PT  - Journal Article
DEP - 20190420
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Astrocytes
MH  - Brain Ischemia/*drug therapy/metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Infarction, Middle Cerebral Artery/*drug therapy/metabolism
MH  - Neurogenesis/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vascular Endothelial Growth Factor A/*metabolism
OTO - NOTNLM
OT  - BDNF
OT  - NPCs
OT  - Neurogenesis
OT  - VEGF
OT  - Yangyin Tongnao granules
EDAT- 2019/04/22 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/04/22 06:00
PHST- 2019/01/29 00:00 [received]
PHST- 2019/04/13 00:00 [accepted]
PHST- 2019/04/22 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/04/22 06:00 [entrez]
AID - 10.1007/s11033-019-04824-5 [pii]
AID - 10.1007/s11033-019-04824-5 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Aug;46(4):3817-3826. doi: 10.1007/s11033-019-04824-5. Epub 
      2019 Apr 20.