PMID- 31009323
OWN - NLM
STAT- MEDLINE
DCOM- 20200205
LR  - 20200205
IS  - 1931-8448 (Electronic)
IS  - 1076-6294 (Linking)
VI  - 25
IP  - 7
DP  - 2019 Sep
TI  - Characterization of Plasmid-Mediated Quinolone Resistance in Gram-Negative 
      Bacterial Strains from Animals and Humans in China.
PG  - 1050-1056
LID - 10.1089/mdr.2018.0405 [doi]
AB  - The aim of this study was to determine the molecular epidemiology of 
      plasmid-mediated quinolone resistance (PMQR) determinants in Escherichia coli, 
      Salmonella enterica (Salmonella spp.), Klebsiella pneumoniae, and Proteus 
      mirabilis. Four hundred seventy-two nonrepetitive isolates were collected from 
      different sources in China and screened for the presence of PMQR genes (PMQRs). 
      Then, 49 PMQR producers were selected to study the coexistence of PMQRs and other 
      resistance genes using whole-genome sequencing (WGS). High rates of resistance to 
      tetracycline (93.4%), nalidixic acid (81.5%), and norfloxacin (65.8%) were 
      observed. The predominant PMQRs were aac(6')-Ib-cr (28.6%) and oqxAB (21.4%). The 
      prevalence of PMQR determinants was significantly higher (p < 0.05) in E. coli 
      from stockmen (55.9%, 19/34), pigs (51.1%, 70/137), and laying hens (43.1%, 
      28/65) than that from wild animals (21.7%, 5/23) and dairy cattle (20.1%, 5/24). 
      WGS results showed that 89.8% of the PMQR-positive isolates co-harbored 
      beta-lactamase genes, with bla(CTX-M) being the dominant beta-lactamase gene. In K. 
      pneumoniae, the coexistence rate of oqxAB and qnrB with fosA, bla(DHA-1), and 
      bla(SHV) was significantly higher than that in P. mirabilis and E. coli. In 
      contrast, the coexistence of qnrD and bla(OXA-1) was more prominent (p < 0.001) 
      in P. mirabilis than in the other two species. Particularly, oqxAB and mcr-1 had 
      an obvious preference for E. coli than K. pneumonia and P. mirabilis (p < 0.001), 
      which had not been reported in previous studies on the prevalence of PMQRs.
FAU - Li, Ping
AU  - Li P
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
FAU - Liu, Dan
AU  - Liu D
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
FAU - Zhang, Xiuzhong
AU  - Zhang X
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
FAU - Tuo, Hongmei
AU  - Tuo H
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
FAU - Lei, Changwei
AU  - Lei C
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
FAU - Xie, Xianjun
AU  - Xie X
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
FAU - Gu, Ju
AU  - Gu J
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
FAU - Zhang, Anyun
AU  - Zhang A
AD  - Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Key 
      Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College 
      of Life Sciences, Sichuan University, Chengdu, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190422
PL  - United States
TA  - Microb Drug Resist
JT  - Microbial drug resistance (Larchmont, N.Y.)
JID - 9508567
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Quinolones)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Animals
MH  - Animals, Wild/microbiology
MH  - Anti-Bacterial Agents/therapeutic use
MH  - Chickens
MH  - China
MH  - Drug Resistance, Multiple, Bacterial/*genetics
MH  - Gram-Negative Bacteria/*genetics/*isolation & purification
MH  - Gram-Negative Bacterial Infections/*microbiology
MH  - Humans
MH  - Microbial Sensitivity Tests/methods
MH  - Plasmids/*genetics
MH  - Prevalence
MH  - Quinolones
MH  - Swine
MH  - beta-Lactamases/genetics
OTO - NOTNLM
OT  - PMQR
OT  - WGS
OT  - coexistence
OT  - gram-negative bacterial strains
OT  - mcr-1
OT  - species preference
EDAT- 2019/04/23 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/04/23 06:00
PHST- 2019/04/23 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/04/23 06:00 [entrez]
AID - 10.1089/mdr.2018.0405 [doi]
PST - ppublish
SO  - Microb Drug Resist. 2019 Sep;25(7):1050-1056. doi: 10.1089/mdr.2018.0405. Epub 
      2019 Apr 22.