PMID- 31009874
OWN - NLM
STAT- MEDLINE
DCOM- 20200107
LR  - 20200107
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 171
DP  - 2019 Jul 15
TI  - Multi-class determination of steroid hormones and antibiotics in fatty hotpot 
      ingredients by pressurized liquid extraction and liquid chromatography-tandem 
      mass spectrometry.
PG  - 193-203
LID - S0731-7085(19)30397-8 [pii]
LID - 10.1016/j.jpba.2019.04.019 [doi]
AB  - Due to the misuse of hormones and antibiotics resulting in drug residues in meat 
      products, serious concerns have arisen regarding to the potential risks for human 
      health. In this paper, a simple and efficient method was developed to 
      simultaneously quantify six steroid hormones and six antibiotics in fatty foods 
      by combining pressurized liquid extraction (PLE) with ultra-high performance 
      liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Adsorbents, 
      extraction temperature, extraction time, recycle time and several other 
      parameters were optimized in current investigation. The optimized PLE method was 
      employed by using n-hexane-methanol-acetonitrile (2:1:1, v/v/v) as extraction 
      solvent, silica gel as lipid sorbent, and the optimum parameters were 60  degrees C, 
      1500 psi and 10 min, respectively. Under the optimal conditions, the recoveries 
      of spiked samples ranged from 82.2% to 115.0%. The limits of quantifications 
      (LODs) were from 0.4 to 25.0 mug/kg. The developed PLE method was successfully 
      applied to analyze steroid hormones and antibiotics in 27 fatty foods of hotpot 
      ingredients without any additional clean-up steps. The results showed that the 
      residues of estradiol benzoate (EB) and enrofloxacin (EN) in some matrices were 
      much higher than maximum residue limits (MRLs) value, especially EB in fish, 
      which exceeded 176.7 mug/kg. The results demonstrated that the developed method 
      was sensitive and efficient in testing hotpot ingredients, and was an excellent 
      analytical tool for monitoring residues of steroid hormones and antibiotics in 
      fatty foods.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Wang, Mengting
AU  - Wang M
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe 
      District, 110016, Shenyang, Liaoning Province, PR China.
FAU - Wang, Yang
AU  - Wang Y
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe 
      District, 110016, Shenyang, Liaoning Province, PR China.
FAU - Peng, Bin
AU  - Peng B
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe 
      District, 110016, Shenyang, Liaoning Province, PR China.
FAU - Zhao, Na
AU  - Zhao N
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe 
      District, 110016, Shenyang, Liaoning Province, PR China.
FAU - Zhao, Jing
AU  - Zhao J
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe 
      District, 110016, Shenyang, Liaoning Province, PR China.
FAU - Xiong, Zhili
AU  - Xiong Z
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe 
      District, 110016, Shenyang, Liaoning Province, PR China.
FAU - Zhao, Longshan
AU  - Zhao L
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road Shenhe 
      District, 110016, Shenyang, Liaoning Province, PR China. Electronic address: 
      longshanzhao@163.com.
LA  - eng
PT  - Journal Article
DEP - 20190415
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Dietary Fats)
RN  - 0 (Fats)
RN  - 0 (Veterinary Drugs)
RN  - 1S4CJB5ZGN (estradiol 3-benzoate)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Anti-Bacterial Agents/*analysis
MH  - Chemical Fractionation
MH  - Chromatography, High Pressure Liquid
MH  - Dietary Fats/*analysis
MH  - Drug Residues/*analysis
MH  - Estradiol/*analogs & derivatives/analysis
MH  - Fats/*chemistry
MH  - Food Analysis
MH  - Limit of Detection
MH  - Tandem Mass Spectrometry
MH  - Veterinary Drugs/*analysis
OTO - NOTNLM
OT  - Pressurized liquid extraction
OT  - Ultra-high performance liquid chromatography-tandem mass spectrometry
OT  - Veterinary drugs
EDAT- 2019/04/23 06:00
MHDA- 2020/01/08 06:00
CRDT- 2019/04/23 06:00
PHST- 2019/02/14 00:00 [received]
PHST- 2019/03/26 00:00 [revised]
PHST- 2019/04/09 00:00 [accepted]
PHST- 2019/04/23 06:00 [pubmed]
PHST- 2020/01/08 06:00 [medline]
PHST- 2019/04/23 06:00 [entrez]
AID - S0731-7085(19)30397-8 [pii]
AID - 10.1016/j.jpba.2019.04.019 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2019 Jul 15;171:193-203. doi: 10.1016/j.jpba.2019.04.019. 
      Epub 2019 Apr 15.