PMID- 31010055 OWN - NLM STAT- MEDLINE DCOM- 20190812 LR - 20200225 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 20 IP - 8 DP - 2019 Apr 20 TI - Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats. LID - 10.3390/ijms20081951 [doi] LID - 1951 AB - Hyperbaric oxygen treatment (HBOT) has been used to reduce neuropathic pain. Melatonin and opioid receptors are involved in neuropathic pain, but it is not known if HBOT works through these pathways to achieve its antinociceptive effect. We divided anesthetized rats into two treatment and three sham groups. The two treatment groups received third-degree burns on their right hind paws, one treated in a hyperbaric chamber for a week and the other for two weeks. We evaluated the mechanical paw-withdrawal threshold (MWT) and expression of melatonin receptor 1 (MT1), melatonin receptor 2 (MT2), mu (MOR) and kappa (KOR) opioid receptor, brain-derived neurotrophic factor (BDNF), Substance P, and calcitonin gene-related peptide (CGRP) in cuneate nucleus, dorsal horn, and hind paw skin by immunohistochemical, immunofluorescence assays and real-time quantitative polymerase chain reaction (RT-PCR). The group receiving one-week HBOT had increased expressions of MT1, MT2, MOR and KOR and decreased expressions of BDNF, Substance P, and CGRP. Their mechanically measured pain levels returned to normal within a week and lasted three weeks. This anti-allodynia effect lasted twice as long in those treated for two weeks. Our findings suggest that increasing the duration of HBOT can reduce burn-induced mechanical allodynia for an extended period of time in rats. The upregulation of melatonin and opioid receptors observed after one week of HBOT suggests they may be partly involved in attenuation of the mechanical allodynia. Downregulation of BDNF, substance P and CGRP may have also contributed to the overall beneficial effect of HBOT. FAU - Wu, Zong-Sheng AU - Wu ZS AD - Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. a8905114@gmail.com. FAU - Wu, Sheng-Hua AU - Wu SH AD - Department of Anesthesiology, Kaohsiung Medical University Hospital, 807 Kaohsiung, Taiwan. elsawu2@gmail.com. AD - Department of Anesthesiology, Kaohsiung Municipal Hsiao-Kang Hospital, 807 Kaohsiung, Taiwan. elsawu2@gmail.com. AD - Department of Anesthesiology, School of Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. elsawu2@gmail.com. FAU - Lee, Su-Shin AU - Lee SS AD - Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, 807 Kaohsiung, Taiwan. sushin@kmu.edu.tw. AD - Department of Surgery, School of Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. sushin@kmu.edu.tw. AD - Center for Stem Cell Research, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. sushin@kmu.edu.tw. AD - Orthopaedic Research Center, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. sushin@kmu.edu.tw. FAU - Lin, Cen-Hung AU - Lin CH AD - Department of Plastic & Reconstructive Surgery, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, 807 Kaohsiung, Taiwan. gigilin119@msn.com. FAU - Chang, Chih-Hau AU - Chang CH AD - Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, 807 Kaohsiung, Taiwan. igor8301023@yahoo.com.tw. FAU - Lo, Jing-Jou AU - Lo JJ AD - School of Post-Baccalaureate Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. joekll@hotmail.com. FAU - Chai, Chee-Yin AU - Chai CY AD - Departments of Pathology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. cychai@kmu.edu.tw. FAU - Wu, Ching-Shuang AU - Wu CS AD - Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. m785034@kmu.edu.tw. FAU - Huang, Shu-Hung AU - Huang SH AD - Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, 807 Kaohsiung, Taiwan. huangsh63@gmail.com. AD - Department of Surgery, School of Medicine, College of Medicine, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. huangsh63@gmail.com. AD - Center for Stem Cell Research, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. huangsh63@gmail.com. AD - Orthopaedic Research Center, Kaohsiung Medical University, 807 Kaohsiung, Taiwan. huangsh63@gmail.com. LA - eng GR - 107-2314-B-037 -063 -MY2/the Ministry of Science and Technology of Taiwan/ GR - 106-2314-B-037-028/the Ministry of Science and Technology of Taiwan/ GR - MOHW107-TDU-B-212-123006/Ministry of Health and Welfare/ GR - KMUH107-7R26/Kaohsiung Medical University Hospital/ GR - KMUH107-7R90/Kaohsiung Medical University Hospital/ GR - KMU-Q108011/Kaohsiung Medical University Research Foundation/ PT - Journal Article DEP - 20190420 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (Receptors, Melatonin) RN - 0 (Receptors, Opioid) RN - 33507-63-0 (Substance P) RN - JHB2QIZ69Z (Calcitonin Gene-Related Peptide) MH - Animals MH - Astrocytes/metabolism/pathology MH - Behavior, Animal MH - Brain-Derived Neurotrophic Factor/metabolism MH - Burns/*complications MH - Calcitonin Gene-Related Peptide/metabolism MH - Glial Fibrillary Acidic Protein/metabolism MH - *Hyperbaric Oxygenation MH - Male MH - Medulla Oblongata/metabolism MH - Neuralgia/*etiology/*therapy MH - Nociception MH - Rats, Sprague-Dawley MH - Receptors, Melatonin/metabolism MH - Receptors, Opioid/metabolism MH - Skin/pathology MH - Spinal Cord Dorsal Horn/metabolism MH - Substance P/metabolism PMC - PMC6514672 OTO - NOTNLM OT - cuneate nucleus OT - hyperbaric oxygen OT - melatonin OT - neuropathic pain OT - opioid receptor COIS- The authors declare no conflict of interest. EDAT- 2019/04/24 06:00 MHDA- 2019/08/14 06:00 PMCR- 2019/04/01 CRDT- 2019/04/24 06:00 PHST- 2019/03/01 00:00 [received] PHST- 2019/04/12 00:00 [revised] PHST- 2019/04/18 00:00 [accepted] PHST- 2019/04/24 06:00 [entrez] PHST- 2019/04/24 06:00 [pubmed] PHST- 2019/08/14 06:00 [medline] PHST- 2019/04/01 00:00 [pmc-release] AID - ijms20081951 [pii] AID - ijms-20-01951 [pii] AID - 10.3390/ijms20081951 [doi] PST - epublish SO - Int J Mol Sci. 2019 Apr 20;20(8):1951. doi: 10.3390/ijms20081951.