PMID- 3101055
OWN - NLM
STAT- MEDLINE
DCOM- 19870316
LR  - 20191029
IS  - 0257-2761 (Print)
IS  - 0257-2761 (Linking)
VI  - 5
IP  - 2
DP  - 1986
TI  - Ultrastructural immunolabeling in the diagnosis of light-chain-related renal 
      disease.
PG  - 170-87
AB  - Renal tissue from eight patients with light-chain deposition disease (LCDD) was 
      immunolabeled for electron microscopy by a postembedding indirect immunogold 
      staining procedure, using anti-kappa and anti-lambda antibodies. This technique 
      provided an exact immunomorphological method to confirm the presence of 
      monotypical light chains in glomeruli, tubules, and vessels. In two cases, it 
      served to prove monotypical light-chain deposition which was not clear upon 
      examination of sections stained for kappa and lambda light chains at the light 
      microscopic level, using PAP or immunofluorescence methods. Three cases of 
      amyloid nephropathy, in which the amyloid deposits stained for monospecific light 
      chains and were immunolabeled ultrastructurally, are also described. LCDD 
      manifests with a variety of morphological patterns in the kidney and the 
      pathological definition of this entity has been difficult. Ultrastructural 
      immunolabeling is a useful method to supplement existing techniques in the 
      diagnosis of LCDD and related conditions. One outstanding feature of the 
      postembedding technique is the ability to examine fixed and preserved renal 
      tissue for the presence of light chains on a retrospective basis.
FAU - Herrera, G A
AU  - Herrera GA
FAU - Paul, R
AU  - Paul R
FAU - Turbat-Herrera, E A
AU  - Turbat-Herrera EA
FAU - Work, J
AU  - Work J
FAU - Viale, G
AU  - Viale G
FAU - dell'Orto, P
AU  - dell'Orto P
FAU - Lott, R L
AU  - Lott RL
FAU - Colombi, R
AU  - Colombi R
FAU - Old, C W
AU  - Old CW
FAU - Lyerly, R H
AU  - Lyerly RH
AU  - et al.
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - Switzerland
TA  - Pathol Immunopathol Res
JT  - Pathology and immunopathology research
JID - 8708069
RN  - 0 (Immunoglobulin Light Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Immunoglobulin Light Chains/*analysis
MH  - Kidney/pathology/ultrastructure
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Nephrotic Syndrome/*diagnosis/immunology/pathology
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
AID - 10.1159/000157010 [doi]
PST - ppublish
SO  - Pathol Immunopathol Res. 1986;5(2):170-87. doi: 10.1159/000157010.