PMID- 31013639
OWN - NLM
STAT- MEDLINE
DCOM- 20190813
LR  - 20211204
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 8
DP  - 2019 Apr 22
TI  - Brassinin Represses Invasive Potential of Lung Carcinoma Cells through 
      Deactivation of PI3K/Akt/mTOR Signaling Cascade.
LID - 10.3390/molecules24081584 [doi]
LID - 1584
AB  - The epithelial-mesenchymal transition (EMT) is a phenomenon that facilitates 
      epithelial cells to acquire invasive potential to induce the initiation the 
      metastatic spread of tumor cells. Here, we determined if brassinin (BSN) can 
      affect the EMT process and deciphered its anti-cancer effects. BSN attenuated the 
      levels of EMT linked genes and suppressed transforming growth factor beta 
      (TGF-beta)-mediated regulation of diverse mesenchymal markers. Additionally, BSN did 
      increase the expression of various epithelial marker proteins in lung cancer 
      cells. TGF-beta-induced morphological changes and induction of invasive ability of 
      tumor cells was also found to be abrogated by BSN treatment. Finally, BSN not 
      only suppressed constitutive, but also inducible phosphoinositide-3-kinase 
      (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) 
      phosphorylation in tumor cells.
FAU - Yang, Min Hee
AU  - Yang MH
AD  - KHU-KIST Department of Converging Science and Technology, Kyung Hee University, 
      Seoul 02447, Korea. didmini@naver.com.
FAU - Lee, Jong Hyun
AU  - Lee JH
AD  - Department of Science in Korean Medicine, Kyung Hee University, 24 
      Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea. mirue88@nate.com.
FAU - Ko, Jeong-Hyeon
AU  - Ko JH
AD  - Department of Science in Korean Medicine, Kyung Hee University, 24 
      Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea. gokjh1647@gmail.com.
FAU - Jung, Sang Hoon
AU  - Jung SH
AD  - KHU-KIST Department of Converging Science and Technology, Kyung Hee University, 
      Seoul 02447, Korea. shjung507@gmail.com.
FAU - Sethi, Gautam
AU  - Sethi G
AD  - Department of Pharmacology, Yong Loo Lin School of Medicine, National University 
      of Singapore, Singapore 117600, Singapore. phcgs@nus.edu.sg.
FAU - Ahn, Kwang Seok
AU  - Ahn KS
AUID- ORCID: 0000-0002-2882-0612
AD  - KHU-KIST Department of Converging Science and Technology, Kyung Hee University, 
      Seoul 02447, Korea. ksahn@khu.ac.kr.
AD  - Department of Science in Korean Medicine, Kyung Hee University, 24 
      Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea. ksahn@khu.ac.kr.
LA  - eng
GR  - NRF-2018R1D1A1B07042969 and 2017M3A9E4065333/National Research Foundation of 
      Korea/
PT  - Journal Article
DEP - 20190422
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Indoles)
RN  - 0 (Thiocarbamates)
RN  - 105748-59-2 (brassinin)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
MH  - A549 Cells
MH  - Epithelial-Mesenchymal Transition/*drug effects
MH  - Humans
MH  - Indoles/*pharmacology
MH  - Lung Neoplasms/*drug therapy/*metabolism/pathology
MH  - Phosphatidylinositol 3-Kinase/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Signal Transduction/*drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Thiocarbamates/*pharmacology
PMC - PMC6514890
OTO - NOTNLM
OT  - EMT
OT  - PI3K/Akt/mTOR
OT  - brassinin
OT  - lung carcinoma
COIS- The authors declare no conflict of interest.
EDAT- 2019/04/25 06:00
MHDA- 2019/08/14 06:00
PMCR- 2019/04/22
CRDT- 2019/04/25 06:00
PHST- 2019/04/15 00:00 [received]
PHST- 2019/04/19 00:00 [revised]
PHST- 2019/04/19 00:00 [accepted]
PHST- 2019/04/25 06:00 [entrez]
PHST- 2019/04/25 06:00 [pubmed]
PHST- 2019/08/14 06:00 [medline]
PHST- 2019/04/22 00:00 [pmc-release]
AID - molecules24081584 [pii]
AID - molecules-24-01584 [pii]
AID - 10.3390/molecules24081584 [doi]
PST - epublish
SO  - Molecules. 2019 Apr 22;24(8):1584. doi: 10.3390/molecules24081584.