PMID- 31015332
OWN - NLM
STAT- MEDLINE
DCOM- 20190620
LR  - 20200514
IS  - 2150-7511 (Electronic)
VI  - 10
IP  - 2
DP  - 2019 Apr 23
TI  - Functional Specialization in Vibrio cholerae Diguanylate Cyclases: Distinct Modes 
      of Motility Suppression and c-di-GMP Production.
LID - 10.1128/mBio.00670-19 [doi]
LID - e00670-19
AB  - Vibrio cholerae biofilm formation and associated motility suppression are 
      correlated with increased concentrations of cyclic diguanylate monophosphate 
      (c-di-GMP), which are in turn driven by increased levels and/or activity of 
      diguanylate cyclases (DGCs). To further our understanding of how c-di-GMP 
      modulators in V. cholerae individually and collectively influence motility with 
      cellular resolution, we determined how DGCs CdgD and CdgH impact intracellular 
      c-di-GMP levels, motility, and biofilm formation. Our results indicated that CdgH 
      strongly influences swim speed distributions; cells in which cdgH was deleted had 
      higher average swim speeds than wild-type cells. Furthermore, our results suggest 
      that CdgD, rather than CdgH, is the dominant DGC responsible for postattachment 
      c-di-GMP production in biofilms. Lipopolysaccharide (LPS) biosynthesis genes were 
      found to be extragenic bypass suppressors of the motility phenotypes of strains 
      DeltacdgD and DeltacdgH We compared the motility regulation mechanism of the DGCs with 
      that of Gmd, an LPS O-antigen biosynthesis protein, and discovered that 
      comodulation of c-di-GMP levels by these motility effectors can be positively or 
      negatively cooperative rather than simply additive. Taken together, these results 
      suggest that different environmental and metabolic inputs orchestrate DGC 
      responses of V. cholerae via c-di-GMP production and motility 
      modulation.IMPORTANCE Cyclic diguanylate monophosphate (c-di-GMP) is a broadly 
      conserved bacterial signaling molecule that affects motility, biofilm formation, 
      and virulence. Although it has been known that high intracellular concentrations 
      of c-di-GMP correlate with motility suppression and biofilm formation, how the 53 
      predicted c-di-GMP modulators in Vibrio cholerae collectively influence motility 
      is not understood in detail. Here we used a combination of plate assays and 
      single-cell tracking methods to correlate motility and biofilm formation outcomes 
      with specific enzymes involved in c-di-GMP synthesis in Vibrio cholerae, the 
      causative agent of the disease cholera.
CI  - Copyright (c) 2019 Zamorano-Sanchez et al.
FAU - Zamorano-Sanchez, David
AU  - Zamorano-Sanchez D
AD  - Department of Microbiology and Environmental Toxicology, University of 
      California, Santa Cruz, Santa Cruz, California, USA.
FAU - Xian, Wujing
AU  - Xian W
AD  - Department of Bioengineering, University of California, Los Angeles, Los Angeles, 
      California, USA.
FAU - Lee, Calvin K
AU  - Lee CK
AD  - Department of Bioengineering, University of California, Los Angeles, Los Angeles, 
      California, USA.
FAU - Salinas, Mauro
AU  - Salinas M
AD  - Department of Microbiology and Environmental Toxicology, University of 
      California, Santa Cruz, Santa Cruz, California, USA.
FAU - Thongsomboon, Wiriya
AU  - Thongsomboon W
AD  - Department of Chemistry, Stanford University, Stanford, California, USA.
FAU - Cegelski, Lynette
AU  - Cegelski L
AD  - Department of Chemistry, Stanford University, Stanford, California, USA.
FAU - Wong, Gerard C L
AU  - Wong GCL
AD  - Department of Bioengineering, University of California, Los Angeles, Los Angeles, 
      California, USA gclwong.ucla@gmail.com fyildiz@ucsc.edu.
AD  - Department of Chemistry and Biochemistry, University of California, Los Angeles, 
      Los Angeles, California, USA.
AD  - California Nano Systems Institute, University of California, Los Angeles, Los 
      Angeles, California, USA.
FAU - Yildiz, Fitnat H
AU  - Yildiz FH
AD  - Department of Microbiology and Environmental Toxicology, University of 
      California, Santa Cruz, Santa Cruz, California, USA gclwong.ucla@gmail.com 
      fyildiz@ucsc.edu.
LA  - eng
GR  - R01 AI102584/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190423
PL  - United States
TA  - mBio
JT  - mBio
JID - 101519231
RN  - 0 (Escherichia coli Proteins)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - EC 4.6.- (Phosphorus-Oxygen Lyases)
RN  - EC 4.6.1.- (diguanylate cyclase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
EIN - mBio. 2020 Sep 22;11(5):. PMID: 32963008
MH  - Biofilms/*growth & development
MH  - Cyclic GMP/*analogs & derivatives/metabolism
MH  - Escherichia coli Proteins/genetics/*metabolism
MH  - Gene Deletion
MH  - Gene Expression Regulation, Bacterial
MH  - Locomotion
MH  - Phosphorus-Oxygen Lyases/genetics/*metabolism
MH  - Vibrio cholerae/*enzymology/genetics/*physiology
PMC - PMC6479008
OTO - NOTNLM
OT  - Vibrio cholerae
OT  - biofilm
OT  - c-di-GMP
OT  - motility
EDAT- 2019/04/25 06:00
MHDA- 2019/06/21 06:00
PMCR- 2019/04/23
CRDT- 2019/04/25 06:00
PHST- 2019/04/25 06:00 [entrez]
PHST- 2019/04/25 06:00 [pubmed]
PHST- 2019/06/21 06:00 [medline]
PHST- 2019/04/23 00:00 [pmc-release]
AID - mBio.00670-19 [pii]
AID - mBio00670-19 [pii]
AID - 10.1128/mBio.00670-19 [doi]
PST - epublish
SO  - mBio. 2019 Apr 23;10(2):e00670-19. doi: 10.1128/mBio.00670-19.