PMID- 31015841
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220408
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2019
DP  - 2019
TI  - Exosomes from Human Umbilical Cord Mesenchymal Stem Cells Reduce Damage from 
      Oxidative Stress and the Epithelial-Mesenchymal Transition in Renal Epithelial 
      Cells Exposed to Oxalate and Calcium Oxalate Monohydrate.
PG  - 6935806
LID - 10.1155/2019/6935806 [doi]
LID - 6935806
AB  - OBJECTIVE: To investigate whether exosomes from human umbilical cord mesenchymal 
      stem cells (hUC-MSCs) can protect against the toxic effects of oxalate and 
      calcium oxalate monohydrate (COM) crystals in human proximal tubular epithelial 
      (HK-2) cells. METHODS: Exosomes were isolated from hUC-MSCs, purified by 
      ultracentrifugation, and verified by examination of cell morphology using 
      transmission electron microscopy and the presence of specific biomarkers. HK-2 
      cells received 1 of 4 treatments: control (cells alone), hUC-MSC exosomes, 
      oxalate+COM, or oxalate+COM and hUC-MSC exosomes. Cell viability was determined 
      using the MTT assay. Oxidative stress was determined by measuring LDH activity 
      and the levels of H(2)O(2), malondialdehyde (MDA), and reactive oxygen species 
      (ROS). Expressions of N-cadherin, TGF-beta, and ZO-1 were determined by 
      immunofluorescence. Expressions of epithelial markers, mesenchymal markers, and 
      related signaling pathway proteins were determined by western blotting. RESULTS: 
      After 48 h, cells in the oxalate+COM group lost their adhesion, appeared long, 
      spindle-shaped, and scattered, and the number of cells had significantly 
      decreased. The oxalate+COM treatment also upregulated TGF-beta and mesenchymal 
      markers, downregulated epithelial markers, increased the levels of LDH, H(2)O(2), 
      MDA, and ROS, decreased cell viability, and increased cell migration. The 
      isolated exosomes had double-layer membranes, had hollow, circular, or elliptical 
      shapes, had diameters mostly between 30 and 100 nm, and expressed CD9, CD63, and 
      Alix. Treatment of HK-2 cells with hUC-MSC exosomes reversed or partly reversed 
      all the effects of oxalate+COM. CONCLUSIONS: Exosomes from hUC-MSCs alleviate the 
      oxidative injury and the epithelial-mesenchymal transformation of HK-2 cells that 
      is induced by oxalate+COM.
FAU - Li, Dian
AU  - Li D
AUID- ORCID: 0000-0002-8350-8648
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Zhang, Dan
AU  - Zhang D
AUID- ORCID: 0000-0003-4590-337X
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Tang, Bo
AU  - Tang B
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Zhou, Yue
AU  - Zhou Y
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Guo, Wenhao
AU  - Guo W
AUID- ORCID: 0000-0003-1717-7585
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Kang, Qing
AU  - Kang Q
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Wang, Zhang
AU  - Wang Z
AUID- ORCID: 0000-0002-6661-7674
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Shen, Lianju
AU  - Shen L
AUID- ORCID: 0000-0001-8973-5393
AD  - Chongqing Key Laboratory of Child Urogenital Development and Tissue Engineering, 
      Ministry of Education Key Laboratory of Child Development and Disorders, China 
      International Science and Technology Cooperation Base of Child Development and 
      Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, 
      China.
FAU - Wei, Guanghui
AU  - Wei G
AUID- ORCID: 0000-0002-7822-8684
AD  - Department of Urology, Children's Hospital of Chongqing Medical University, 
      Chongqing 400014, China.
FAU - He, Dawei
AU  - He D
AUID- ORCID: 0000-0003-1548-424X
AD  - Department of Urology, Children's Hospital of Chongqing Medical University, 
      Chongqing 400014, China.
LA  - eng
PT  - Journal Article
DEP - 20190319
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC6444261
EDAT- 2019/04/25 06:00
MHDA- 2019/04/25 06:01
PMCR- 2019/03/19
CRDT- 2019/04/25 06:00
PHST- 2018/12/01 00:00 [received]
PHST- 2019/01/24 00:00 [revised]
PHST- 2019/02/14 00:00 [accepted]
PHST- 2019/04/25 06:00 [entrez]
PHST- 2019/04/25 06:00 [pubmed]
PHST- 2019/04/25 06:01 [medline]
PHST- 2019/03/19 00:00 [pmc-release]
AID - 10.1155/2019/6935806 [doi]
PST - epublish
SO  - Stem Cells Int. 2019 Mar 19;2019:6935806. doi: 10.1155/2019/6935806. eCollection 
      2019.