PMID- 31016708
OWN - NLM
STAT- MEDLINE
DCOM- 20190906
LR  - 20190906
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1973
DP  - 2019
TI  - The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons.
PG  - 261-279
LID - 10.1007/978-1-4939-9216-4_17 [doi]
AB  - Molecular beacons composed of the artificial serinol nucleic acid (SNA) have 
      demonstrated utility as novel fluorescence probes for visualization of RNA in 
      fixed cells using both conventional fluorescence in situ hybridization (FISH) and 
      wash-free FISH protocols. The SNA molecular beacons have higher affinity for 
      target RNA and greater sensitivity than molecular beacons composed of DNA. Here 
      we describe facile synthesis of the SNA using a conventional DNA synthesizer and 
      protocols for purification by PAGE and HPLC as well as methods for use of the SNA 
      molecular beacon in FISH.
FAU - Murayama, Keiji
AU  - Murayama K
AD  - Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Nagoya, Japan.
FAU - Kashida, Hiromu
AU  - Kashida H
AD  - Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Nagoya, Japan.
AD  - PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.
FAU - Asanuma, Hiroyuki
AU  - Asanuma H
AD  - Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya 
      University, Nagoya, Japan. asanuma@chembio.nagoya-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Nucleic Acids)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Propanolamines)
RN  - 0 (Propylene Glycols)
RN  - 63231-63-0 (RNA)
RN  - IC94L30J8M (serinol)
SB  - IM
MH  - Fluorescent Dyes/*chemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Hybridization
MH  - Nucleic Acids/*chemistry
MH  - Oligonucleotide Probes/*chemistry
MH  - Propanolamines/*chemistry
MH  - Propylene Glycols/*chemistry
MH  - RNA/*analysis
OTO - NOTNLM
OT  - Artificial nucleic acid
OT  - DNA
OT  - FISH
OT  - Fluorescent probe
OT  - Molecular beacon
OT  - RNA imaging
OT  - SNA
EDAT- 2019/04/25 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/04/25 06:00
PHST- 2019/04/25 06:00 [entrez]
PHST- 2019/04/25 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
AID - 10.1007/978-1-4939-9216-4_17 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1973:261-279. doi: 10.1007/978-1-4939-9216-4_17.