PMID- 31017523
OWN - NLM
STAT- MEDLINE
DCOM- 20190912
LR  - 20200205
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 83
IP  - 9
DP  - 2019 Sep
TI  - Citrus peel flavonoids improve lipid metabolism by inhibiting miR-33 and miR-122 
      expression in HepG2 cells.
PG  - 1747-1755
LID - 10.1080/09168451.2019.1608807 [doi]
AB  - Citrus plants are rich in flavonoids and beneficial for lipid metabolism. 
      However, the mechanism has not been fully elucidated. Both citrus peel flavonoid 
      extracts (CPFE) and a mixture of their primary flavonoid compounds, namely, 
      nobiletin, tangeretin and hesperidin, citrus flavonoid purity mixture (CFPM), 
      were found to have lipid-lowering effects on oleic acid-induced lipid 
      accumulation in HepG2 cells. The carnitine palmitoyltransferase 1alpha (CPT1alpha) gene 
      was markedly increased, while the fatty acid synthase (FAS) gene was 
      significantly decreased by both CPFE and CFPM in oleic acid-treated HepG2 cells. 
      Flavonoid compounds from citrus peel suppressed miR-122 and miR-33 expression, 
      which were induced by oleic acid. Changes in miR-122 and miR-33 expression, which 
      subsequently affect the expression of their target mRNAs FAS and CPT1alpha, are most 
      likely the principal mechanisms leading to decreased lipid accumulation in HepG2 
      cells. Citrus flavonoids likely regulate lipid metabolism by modulating the 
      expression levels of miR-122 and miR-33.
FAU - Su, Dongxiao
AU  - Su D
AD  - a School of Chemistry and Chemical Engineering, Guangzhou University , Guangzhou 
      , China.
AD  - b Zhejiang Provincial Top Discipline of Biological Engineering (Level A), 
      Zhejiang Wanli University , Ningbo , China.
FAU - Liu, Hesheng
AU  - Liu H
AD  - b Zhejiang Provincial Top Discipline of Biological Engineering (Level A), 
      Zhejiang Wanli University , Ningbo , China.
AD  - c College of Biological and Environmental Sciences, Zhejiang Wanli University , 
      Ningbo , China.
FAU - Qi, Xiangyang
AU  - Qi X
AD  - b Zhejiang Provincial Top Discipline of Biological Engineering (Level A), 
      Zhejiang Wanli University , Ningbo , China.
AD  - c College of Biological and Environmental Sciences, Zhejiang Wanli University , 
      Ningbo , China.
FAU - Dong, Lihong
AU  - Dong L
AD  - d Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural 
      Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture , Guangzhou 
      , P.R. China.
FAU - Zhang, Ruifen
AU  - Zhang R
AD  - d Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural 
      Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture , Guangzhou 
      , P.R. China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - b Zhejiang Provincial Top Discipline of Biological Engineering (Level A), 
      Zhejiang Wanli University , Ningbo , China.
AD  - c College of Biological and Environmental Sciences, Zhejiang Wanli University , 
      Ningbo , China.
LA  - eng
PT  - Journal Article
DEP - 20190424
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Flavonoids)
RN  - 0 (MIRN122 microRNA, human)
RN  - 0 (MIRN33a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Plant Extracts)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - EC 2.3.1.85 (FASN protein, human)
RN  - EC 2.3.1.85 (Fatty Acid Synthase, Type I)
SB  - IM
MH  - Citrus/*chemistry
MH  - Down-Regulation/drug effects
MH  - Fatty Acid Synthase, Type I/genetics
MH  - Flavonoids/*pharmacology
MH  - Hep G2 Cells
MH  - Humans
MH  - Lipid Metabolism/*drug effects
MH  - Lipogenesis/genetics
MH  - MicroRNAs/*genetics
MH  - Oleic Acid/pharmacology
MH  - Plant Extracts/*pharmacology
MH  - Real-Time Polymerase Chain Reaction
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - Citrus
OT  - HepG2
OT  - flavonoid
OT  - lipid metabolism
OT  - miRNA
EDAT- 2019/04/25 06:00
MHDA- 2019/09/13 06:00
CRDT- 2019/04/25 06:00
PHST- 2019/04/25 06:00 [pubmed]
PHST- 2019/09/13 06:00 [medline]
PHST- 2019/04/25 06:00 [entrez]
AID - 10.1080/09168451.2019.1608807 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2019 Sep;83(9):1747-1755. doi: 
      10.1080/09168451.2019.1608807. Epub 2019 Apr 24.