PMID- 31017665
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
DP  - 2019 Apr 24
TI  - Downregulation of miRNA-30a enhanced autophagy in osthole-alleviated myocardium 
      ischemia/reperfusion injury.
LID - 10.1002/jcp.28556 [doi]
AB  - Osthole could alleviate myocardial ischemia/reperfusion (I/R) injury. However, 
      the underlying mechanism remains unclear. In this study, we explored whether 
      microRNA (miR)-30a and its target autophagy marker Beclin-1 involved in the 
      osthole protective role in the rat and cells myocardial I/R injury models. The 
      myocardial damages including increases in myocardial collagen content and cell 
      apoptosis in I/R injury model were observed by Masson's Trichrome Staining and 
      terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assays. 
      Osthole significantly inhibited the myocardial damages. Osthole inhibited the 
      induction of miR-30a expression by I/R in rat and hypoxia/reoxygenation (H/R) in 
      myocardial cells. After knockdown, the expression of miR-30a by miR-30a 
      inhibitor, H/R induced cell apoptosis was significantly inhibited. The level of 
      Beclin-1 expression and ratio of LC3BII/LC3BI were inhibited by I/R in rat and 
      H/R in myocardial cells, whereas osthole significantly increased them. Knockdown 
      of miR-30a significantly upregulated the Beclin-1 expression and ratio of 
      LC3BII/LC3BI. Inhibition of autophagy by 3-MA significantly reversed the 
      protective role of osthole in H/R myocardial cell. Therefore, we concluded that 
      the mechanism by which osthole alleviate myocardial I/R injury may be achieved by 
      enhancing the autophagy partially via inhibiting the expression of miR-30a.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Liu, Shengzhong
AU  - Liu S
AD  - Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 
      Chengdu, Sichuan, China.
AD  - Cardiac Surgery Center, Sichuan Academy of Medical Sciences & Sichuan Provincial 
      People's Hospital, Chengdu, China.
FAU - He, Ying
AU  - He Y
AD  - Psychosomatic Medicine Center, Sichuan Academy of Medical Sciences & Sichuan 
      Provincial People's Hospital, Chengdu, China.
FAU - Shi, Jun
AU  - Shi J
AD  - Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 
      Chengdu, Sichuan, China.
FAU - Liu, Lulu
AU  - Liu L
AD  - Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 
      Chengdu, Sichuan, China.
FAU - Ma, Hao
AU  - Ma H
AD  - Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 
      Chengdu, Sichuan, China.
FAU - He, Li
AU  - He L
AD  - Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 
      Chengdu, Sichuan, China.
FAU - Guo, Yingqiang
AU  - Guo Y
AUID- ORCID: 0000-0001-8385-5116
AD  - Department of Cardiovascular Surgery, West China Hospital, Sichuan University, 
      Chengdu, Sichuan, China.
LA  - eng
GR  - 2018SZ0266/Sichuan Science and Technology Department/
PT  - Journal Article
DEP - 20190424
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
OTO - NOTNLM
OT  - autophagy
OT  - miR-30a
OT  - myocardial ischemia/reperfusion injury
OT  - osthole
EDAT- 2019/04/25 06:00
MHDA- 2019/04/25 06:00
CRDT- 2019/04/25 06:00
PHST- 2018/12/25 00:00 [received]
PHST- 2019/02/23 00:00 [revised]
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/04/25 06:00 [entrez]
PHST- 2019/04/25 06:00 [pubmed]
PHST- 2019/04/25 06:00 [medline]
AID - 10.1002/jcp.28556 [doi]
PST - aheadofprint
SO  - J Cell Physiol. 2019 Apr 24. doi: 10.1002/jcp.28556.