PMID- 31018
OWN - NLM
STAT- MEDLINE
DCOM- 19790126
LR  - 20190819
IS  - 0039-128X (Print)
IS  - 0039-128X (Linking)
VI  - 32
IP  - 3
DP  - 1978 Oct
TI  - 3beta-Hydroxysteroid dehydrogenase activity in human fetal membranes.
PG  - 365-72
AB  - A 3beta-hydroxysteroid dehydrogenase (3betaHSD) was demonstrated in term human 
      fetal membranes (chorion and amnion) with both dehydroepiandrosterone 
      (3beta-hydroxy-5-androsten-17-one) and pregnenolone 
      (3beta-hydroxy-5-pregnen-20-one as substrates, and the subcellular distribution 
      substrate and nucleotide specificity of the enzyme was studied. In both membranes 
      the microsomal fraction (particles which sedimented at 105,000 g after 90 min) 
      had the highest specific activity. The chorion was more active than the amnion 
      but the enzyme in both tissues had similar substrate and nucleotide specificity. 
      NAD was the preferred cofactor, and pregnenolone was a better substrate than 
      dehydroepiandrosterone in the presence of NAD. However, with NADP as cofactor 
      both steroids were equally good substrates. When the 3beta-hydroxysteroid 
      dehydrogenase activity of chorion microsomes was compared with that of placental 
      microsomes, the specific activities were found to be of the same order of 
      magnitude, and the substrate, nucleotide specificity and steroid binding 
      properties were almost identical.
FAU - Gibb, W
AU  - Gibb W
FAU - Lavoie, J C
AU  - Lavoie JC
FAU - Roux, J F
AU  - Roux JF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Steroids
JT  - Steroids
JID - 0404536
RN  - 0U46U6E8UK (NAD)
RN  - 459AG36T1B (Dehydroepiandrosterone)
RN  - 53-59-8 (NADP)
RN  - 73R90F7MQ8 (Pregnenolone)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*metabolism
MH  - Amnion/*enzymology
MH  - Chorion/*enzymology
MH  - Dehydroepiandrosterone
MH  - Female
MH  - Humans
MH  - In Vitro Techniques
MH  - Microsomes/enzymology
MH  - NAD/metabolism
MH  - NADP/metabolism
MH  - Placenta/enzymology
MH  - Pregnancy
MH  - Pregnenolone
MH  - Subcellular Fractions/enzymology
MH  - Substrate Specificity
EDAT- 1978/10/01 00:00
MHDA- 1978/10/01 00:01
CRDT- 1978/10/01 00:00
PHST- 1978/10/01 00:00 [pubmed]
PHST- 1978/10/01 00:01 [medline]
PHST- 1978/10/01 00:00 [entrez]
AID - 0039-128X(78)90088-0 [pii]
AID - 10.1016/0039-128x(78)90088-0 [doi]
PST - ppublish
SO  - Steroids. 1978 Oct;32(3):365-72. doi: 10.1016/0039-128x(78)90088-0.