PMID- 31018729
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1476-4954 (Electronic)
IS  - 1476-4954 (Linking)
VI  - 34
IP  - 4
DP  - 2021 Feb
TI  - HLA-C promotes proliferation and cell cycle progression in trophoblast cells.
PG  - 512-518
LID - 10.1080/14767058.2019.1611772 [doi]
AB  - Objectives: The development of maternal-fetal immune tolerance and adequate 
      trophoblast function are essential for the establishment and maintenance of 
      pregnancy. Human leukocyte antigen (HLA), the major histocompatibility complex 
      (MHC) antigen specific to humans, plays an important role in placentation and is 
      involved in many pregnancy-associated diseases. HLA-C is the only classical MHC I 
      gene expressed at the maternal-fetal interface. To investigate whether HLA-C 
      plays an independent role in regulating the development of trophoblasts, we 
      explored the effect of HLA-C expression on placental development.Methods: The 
      role of HLA-C in the growth and migration of trophoblast JAR and HTR-8/Svneo cell 
      lines was investigated after HLA-C-expressing lentivirus transfection.Results: 
      The MTT assay and colony formation assay showed that HLA-C promoted cell 
      proliferation. Furthermore, cell cycle analysis showed that HLA-C overexpression 
      accelerated the transition of trophoblast cells from the G0/G1 phase to the S 
      phase. However, FACS analysis and migration assay indicated that HLA-C had no 
      significant influence on trophoblast apoptosis and migration.Conclusion: Our 
      study demonstrated for the first time that besides being involved in immune 
      tolerance, HLA-C can directly promote placental growth without interacting with 
      immune cells, which could provide a new insight into studying the functions of 
      HLA-C at the maternal-fetal interface.
FAU - Lv, Hong
AU  - Lv H
AD  - Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, China.
FAU - Zhou, Qian
AU  - Zhou Q
AD  - Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated 
      to Shandong University, Jinan, China.
AD  - Shandong Provincial Hospital Affiliated to Shandong First Medical University, 
      Jinan, China.
FAU - Li, Lie
AU  - Li L
AD  - Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated 
      to Shandong University, Jinan, China.
AD  - Shandong Provincial Hospital Affiliated to Shandong First Medical University, 
      Jinan, China.
FAU - Wang, Shan
AU  - Wang S
AD  - Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated 
      to Shandong University, Jinan, China.
AD  - Shandong Provincial Hospital Affiliated to Shandong First Medical University, 
      Jinan, China.
LA  - eng
PT  - Journal Article
DEP - 20190515
PL  - England
TA  - J Matern Fetal Neonatal Med
JT  - The journal of maternal-fetal & neonatal medicine : the official journal of the 
      European Association of Perinatal Medicine, the Federation of Asia and Oceania 
      Perinatal Societies, the International Society of Perinatal Obstetricians
JID - 101136916
RN  - 0 (HLA-C Antigens)
SB  - IM
MH  - Cell Cycle
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - HLA-C Antigens/genetics
MH  - Humans
MH  - *Placenta
MH  - Pregnancy
MH  - *Trophoblasts
OTO - NOTNLM
OT  - Cell cycle
OT  - HLA-C
OT  - placentation
OT  - proliferation
OT  - trophoblast cell
EDAT- 2019/04/26 06:00
MHDA- 2021/06/22 06:00
CRDT- 2019/04/26 06:00
PHST- 2019/04/26 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2019/04/26 06:00 [entrez]
AID - 10.1080/14767058.2019.1611772 [doi]
PST - ppublish
SO  - J Matern Fetal Neonatal Med. 2021 Feb;34(4):512-518. doi: 
      10.1080/14767058.2019.1611772. Epub 2019 May 15.