PMID- 31019210
OWN - NLM
STAT- MEDLINE
DCOM- 20201019
LR  - 20210109
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Apr 24
TI  - Isolation, Purification, and Characterization of Heparinase from Streptomyces 
      variabilis MTCC 12266.
PG  - 6482
LID - 10.1038/s41598-019-42740-7 [doi]
LID - 6482
AB  - Arterial/venous thrombosis is the major cardiovascular disorder accountable for 
      substantial mortality; and the current demand for antithrombotic agents is 
      extensive. Heparinases depolymerize unfractionated heparin (UFH) for the 
      production of low molecular-weight heparins (LMWHs; used as anticoagulants 
      against thrombosis). A microbial strain of Streptomyces sp. 
      showing antithrombotic activity was isolated from the soil sample collected from 
      north India. The strain was characterized by using 16S rRNA homology technique 
      and identified as Streptomyces variabilis MTCC 12266 capable of producing 
      heparinase enzyme. This is the very first communication reporting Streptomyces 
      genus as the producer of heparinase. It was observed that the production 
      of intracellular heparinase was [63.8 U/mg protein 
      (specific activity)] 1.58 folds higher compared to extracellular heparinase 
      [40.28 U/mg protein]. DEAE-Sephadex A-50 column followed by Sepharose-6B column 
      purification of the crude protein resulted 19.18 folds purified heparinase. 
      SDS-PAGE analysis of heparinase resulted an estimated molecular-weight of 42 kDa. 
      It was also found that intracellular heparinase has the ability to depolymerize 
      heparin to generate LMWHs. Further studies related to the mechanistic action, 
      structural details, and genomics involved in heparinase production 
      from Streptomyces variabilis are warranted for large scale 
      production/purification optimization of heparinase 
      for antithrombotic applications.
FAU - Singh, Vineeta
AU  - Singh V
AD  - Microbiology Division, CSIR-Central Drug Research Institute, Lucknow, 226031, 
      Uttar Pradesh, India. vscdri@gmail.com.
AD  - Department of Biotechnology, Institute of Engineering & Technology, Dr. A.P.J. 
      Abdul Kalam Technical University, Sitapur Road, Lucknow, 226021, Uttar Pradesh, 
      India. vscdri@gmail.com.
FAU - Haque, Shafiul
AU  - Haque S
AUID- ORCID: 0000-0002-2989-121X
AD  - Research and Scientific Studies Unit, College of Nursing & Allied Health 
      Sciences, Jazan University, Jazan, 45142, Saudi Arabia.
FAU - Kumari, Vibha
AU  - Kumari V
AD  - Microbiology Division, CSIR-Central Drug Research Institute, Lucknow, 226031, 
      Uttar Pradesh, India.
FAU - El-Enshasy, Hesham A
AU  - El-Enshasy HA
AD  - Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), 81310 
      UTM, Skudai, Malaysia.
FAU - Mishra, B N
AU  - Mishra BN
AD  - Department of Biotechnology, Institute of Engineering & Technology, Dr. A.P.J. 
      Abdul Kalam Technical University, Sitapur Road, Lucknow, 226021, Uttar Pradesh, 
      India.
FAU - Somvanshi, Pallavi
AU  - Somvanshi P
AUID- ORCID: 0000-0003-1214-9374
AD  - Department of Biotechnology, TERI School of Advanced Studies, Plot No. 10 
      Institutional Area, Vasant Kunj, New Delhi, 110070, India.
FAU - Tripathi, C K M
AU  - Tripathi CKM
AD  - Department of Biotechnology, Shri Ramswaroop Memorial University, Lucknow, 
      225003, Uttar Pradesh, India.
LA  - eng
PT  - Journal Article
DEP - 20190424
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Bacterial Proteins)
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 39455-31-7 (DEAE-Sephadex A-50)
RN  - 9005-49-6 (Heparin)
RN  - 9012-36-6 (Sepharose)
RN  - 9015-73-0 (DEAE-Dextran)
RN  - EC 4.2.2.7 (Heparin Lyase)
RN  - Streptomyces variabilis
SB  - IM
MH  - Bacterial Proteins/isolation & purification/*metabolism
MH  - Chromatography/methods
MH  - DEAE-Dextran/analogs & derivatives
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fibrinolytic Agents/chemistry/metabolism
MH  - Heparin/chemistry/*metabolism
MH  - Heparin Lyase/isolation & purification/*metabolism
MH  - Heparin, Low-Molecular-Weight/chemistry/*metabolism
MH  - India
MH  - Kinetics
MH  - Molecular Weight
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sepharose
MH  - Soil Microbiology
MH  - Streptomyces/classification/genetics/*metabolism
MH  - Substrate Specificity
PMC - PMC6482181
COIS- The authors declare no competing interests.
EDAT- 2019/04/26 06:00
MHDA- 2020/10/21 06:00
PMCR- 2019/04/24
CRDT- 2019/04/26 06:00
PHST- 2018/12/13 00:00 [received]
PHST- 2019/04/01 00:00 [accepted]
PHST- 2019/04/26 06:00 [entrez]
PHST- 2019/04/26 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/04/24 00:00 [pmc-release]
AID - 10.1038/s41598-019-42740-7 [pii]
AID - 42740 [pii]
AID - 10.1038/s41598-019-42740-7 [doi]
PST - epublish
SO  - Sci Rep. 2019 Apr 24;9(1):6482. doi: 10.1038/s41598-019-42740-7.