PMID- 31022564 OWN - NLM STAT- MEDLINE DCOM- 20200106 LR - 20200106 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 239 DP - 2019 Jul 15 TI - Anti-diabetic and renoprotective effects of Cassiae Semen extract in the streptozotocin-induced diabetic rats. PG - 111904 LID - S0378-8741(19)30378-2 [pii] LID - 10.1016/j.jep.2019.111904 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Cassiae Semen, the dried seed of Cassia obtusifolia L. (Leguminosae), is a traditional Chinese medicine. It has long been used as the treatment of diabetic hyperlipidemia and diabetic constipation in Traditional Chinese Medicine formulae. AIM OF THE STUDY: The present study was designed to investigate the anti-diabetic and renoprotective effects of Cassiae Semen extract (CSE) in streptozotocin (STZ)-induced diabetic rats. MATERIALS AND METHODS: Quality control of CSE was performed using HPLC. CSE were orally administered at 27, 54 and 81 mg/kg dose to high-sucrose-high-fat (HSHF) diet and STZ-induced diabetic rats for 60 days. Body weight, glucose metabolism and lipid metabolism profiles were measured to assess the anti-diabetic effect of CSE. Oxidative stress markers and inflammatory factors were determined using commercial kits. Renal function related parameters were also measured. Histopathological examination of kidney was conducted for the validation of pathological changes in the diabetic rats. Immunohistochemical examination of kidney was measured to investigate the expression of RAGE in renal tissues. RESULTS: Five compounds, including two anthraquinones and three naphtopyrones were simultaneously determined in CSE. Compared with diabetic control, groups treated with CSE exhibited an anti-diabetic effect, including a significant amelioration in body weight, glycemic control, oral glucose tolerance and lipid metabolism (P < 0.01). Moreover, oxidative stress and inflammatory responses decreased after oral administration of CSE (P < 0.01). CSE also showed protective effects on renal functions, decreasing the ratio of kidney/body weight, 24 h urine volume, 24 h urine protein, serum creatinine (Scr) and blood urea nitrogen (BUN) (P < 0.01). Additionally, renal protective effect was also observed in histopathological examination. Immunohistochemical analysis showed that CSE downregulated the expression of RAGE. CONCLUSIONS: It turned out that CSE had both anti-diabetic and renoprotective effects in diabetic rats. CSE can be a potential agent in the treatment of type 2 diabetes mellitus (T2DM) and its complications. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Wang, Qiuyan AU - Wang Q AD - Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China. FAU - Zhou, Jiewen AU - Zhou J AD - Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China. FAU - Xiang, Zhinan AU - Xiang Z AD - Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China. FAU - Tong, Qilin AU - Tong Q AD - Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China. FAU - Pan, Jun AU - Pan J AD - Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China. FAU - Wan, Luosheng AU - Wan L AD - Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China. Electronic address: wanluosheng@hust.edu.cn. FAU - Chen, Jiachun AU - Chen J AD - Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, 430030, China. Electronic address: homespringchen@mail.hust.edu.cn. LA - eng PT - Journal Article DEP - 20190422 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Cytokines) RN - 0 (Hypoglycemic Agents) RN - 0 (Lipids) RN - 0 (Plant Extracts) RN - 0 (Protective Agents) SB - IM MH - Animals MH - *Cassia MH - Cytokines/blood MH - Diabetes Mellitus, Experimental/*drug therapy/metabolism/pathology MH - Diabetes Mellitus, Type 2/*drug therapy/metabolism/pathology MH - Diabetic Nephropathies/*drug therapy/metabolism/pathology MH - Hypoglycemic Agents/*therapeutic use MH - Kidney/drug effects/pathology MH - Lipids/blood MH - Male MH - Plant Extracts/*therapeutic use MH - Protective Agents/*therapeutic use MH - Rats, Wistar MH - Seeds/chemistry OTO - NOTNLM OT - Anti-diabetes OT - Anti-inflammation OT - Anti-oxidation OT - Cassiae semen extract OT - Renoprotection EDAT- 2019/04/26 06:00 MHDA- 2020/01/07 06:00 CRDT- 2019/04/26 06:00 PHST- 2019/01/26 00:00 [received] PHST- 2019/04/08 00:00 [revised] PHST- 2019/04/20 00:00 [accepted] PHST- 2019/04/26 06:00 [pubmed] PHST- 2020/01/07 06:00 [medline] PHST- 2019/04/26 06:00 [entrez] AID - S0378-8741(19)30378-2 [pii] AID - 10.1016/j.jep.2019.111904 [doi] PST - ppublish SO - J Ethnopharmacol. 2019 Jul 15;239:111904. doi: 10.1016/j.jep.2019.111904. Epub 2019 Apr 22.