PMID- 31022614
OWN - NLM
STAT- MEDLINE
DCOM- 20191128
LR  - 20191210
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 172
DP  - 2019 Aug 5
TI  - Development and validation of a highly sensitive LC-MS/MS method for in vitro 
      measurement of histamine concentration.
PG  - 33-41
LID - S0731-7085(19)30070-6 [pii]
LID - 10.1016/j.jpba.2019.04.025 [doi]
AB  - The basophil histamine release test (HRT) is an important in vitro diagnostic 
      assay to evaluate immunoglobin E (IgE)-mediated allergic responses. In this 
      study, a bioanalytical LC-MS/MS method was developed and validated to quantify 
      histamine in the leukocyte suspension from human peripheral blood. The method 
      used pre-column derivatization with phenylisothiocyanate (PITC) and the resulting 
      phenylthiocarbamyl (PTC) histamine was analyzed by positive-ion electrospray 
      ionization using the multiple reaction monitoring mode. Chromatographic 
      separation was achieved using an Imtakt-HT C18 column (2.1 mm x 50 mm, 3.0 mum), 
      with a flow rate of 0.35 mL/min, 2 muL injection, and gradient elution with a 
      mixture of acetonitrile-2 mM ammonium acetate buffer (both containing 0.1% formic 
      acid). The total runtime of the method was 3.0 min including equilibration time. 
      The method had a lower limit of detection of 0.1 ng/mL, and the quantifiable 
      range was 0.1-100 ng/mL in the leukocyte suspension. The intra-day and inter-day 
      precision and accuracy results were within the acceptable limits. It was 
      established that histamine quantification should be performed within 2 h of 
      preparing the leukocyte suspension, and freezing and thawing should be avoided. 
      This method was successfully applied to the diagnosis and evaluation of the 
      pathophysiologic mechanism of respiratory or cutaneous allergic diseases.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Kim, Kwang-Youl
AU  - Kim KY
AD  - Department of Clinical Pharmacology, Inha University Hospital, Inha University 
      School of Medicine, Incheon, Republic of Korea.
FAU - Kwon, Hyun-Jung
AU  - Kwon HJ
AD  - Department of Clinical Pharmacology, Inha University Hospital, Inha University 
      School of Medicine, Incheon, Republic of Korea.
FAU - Cho, Sang-Heon
AU  - Cho SH
AD  - Department of Clinical Pharmacology, Inha University Hospital, Inha University 
      School of Medicine, Incheon, Republic of Korea.
FAU - Nam, Moonsuk
AU  - Nam M
AD  - Department of Clinical Pharmacology, Inha University Hospital, Inha University 
      School of Medicine, Incheon, Republic of Korea; Department of Internal Medicine, 
      Inha University Hospital, Inha University School of Medicine, Incheon, Republic 
      of Korea.
FAU - Kim, Cheol-Woo
AU  - Kim CW
AD  - Department of Clinical Pharmacology, Inha University Hospital, Inha University 
      School of Medicine, Incheon, Republic of Korea; Department of Internal Medicine, 
      Inha University Hospital, Inha University School of Medicine, Incheon, Republic 
      of Korea. Electronic address: cwkim1805@inha.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20190412
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Blood Specimen Collection/*methods
MH  - Chromatography, High Pressure Liquid/methods
MH  - Histamine/*blood/immunology
MH  - Humans
MH  - Hypersensitivity/blood/*diagnosis/immunology
MH  - Leukocytes/chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Basophil histamine release test
OT  - Leukocyte suspension
OT  - Multiple reaction monitoring
OT  - Phenylisothiocyanate
EDAT- 2019/04/26 06:00
MHDA- 2019/11/30 06:00
CRDT- 2019/04/26 06:00
PHST- 2019/01/09 00:00 [received]
PHST- 2019/04/10 00:00 [revised]
PHST- 2019/04/11 00:00 [accepted]
PHST- 2019/04/26 06:00 [pubmed]
PHST- 2019/11/30 06:00 [medline]
PHST- 2019/04/26 06:00 [entrez]
AID - S0731-7085(19)30070-6 [pii]
AID - 10.1016/j.jpba.2019.04.025 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2019 Aug 5;172:33-41. doi: 10.1016/j.jpba.2019.04.025. Epub 
      2019 Apr 12.