PMID- 31023003
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220409
IS  - 2005-3606 (Print)
IS  - 2005-5447 (Electronic)
IS  - 2005-3606 (Linking)
VI  - 12
IP  - 2
DP  - 2019 Jul 31
TI  - Enhanced Anti-Cancer Effects of Conditioned Medium from Hypoxic Human Umbilical 
      Cord-Derived Mesenchymal Stem Cells.
PG  - 291-303
LID - 10.15283/ijsc19002 [doi]
AB  - BACKGROUND AND OBJECTIVES: There have been contradictory reports on the 
      pro-cancer or anti-cancer effects of mesenchymal stem cells. In this study, we 
      investigated whether conditioned medium (CM) from hypoxic human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) (H-CM) showed enhanced anti-cancer 
      effects compared with CM from normoxic hUC-MSCs (N-CM). METHODS AND RESULTS: 
      Compared with N-CM, H-CM not only strongly reduced cell viability and increased 
      apoptosis of human cervical cancer cells (HeLa cells), but also increased 
      caspase-3/7 activity, decreased mitochondrial membrane potential (MMP), and 
      induced cell cycle arrest. In contrast, cell viability, apoptosis, MMP, and cell 
      cycle of human dermal fibroblast (hDFs) were not significantly changed by either 
      CM whereas caspase-3/7 activity was decreased by H-CM. Protein antibody array 
      showed that activin A, Beta IG-H3, TIMP-2, RET, and IGFBP-3 were upregulated in 
      H-CM compared with N-CM. Intracellular proteins that were upregulated by H-CM in 
      HeLa cells were represented by apoptosis and cell cycle arrest terms of 
      biological processes of Gene Ontology (GO), and by cell cycle of Kyoto 
      Encyclopedia of Genes and Genomes (KEGG) pathways. In hDFs, negative regulation 
      of apoptosis in biological process of GO and PI3K-Akt signaling pathway of KEGG 
      pathways were represented. CONCLUSIONS: H-CM showed enhanced anti-cancer effects 
      on HeLa cells but did not influence cell viability or apoptosis of hDFs and these 
      different effects were supported by profiling of secretory proteins in both kinds 
      of CM and intracellular signaling of HeLa cells and hDFs.
FAU - Han, Kyu-Hyun
AU  - Han KH
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Kim, Ae-Kyeong
AU  - Kim AK
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Jeong, Gun-Jae
AU  - Jeong GJ
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Jeon, Hye Ran
AU  - Jeon HR
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
FAU - Bhang, Suk Ho
AU  - Bhang SH
AD  - Sungkyunkwan University School of Chemical Engineering, Suwon, Korea.
FAU - Kim, Dong-Ik
AU  - Kim DI
AD  - Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University 
      School of Medicine, Seoul, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Int J Stem Cells
JT  - International journal of stem cells
JID - 101497587
PMC - PMC6657944
OTO - NOTNLM
OT  - Anti-cancer
OT  - Conditioned medium
OT  - Fibroblasts
OT  - Hypoxia
OT  - Mesenchymal stem cells
COIS- Potential Conflict of Interest The authors have no conflicting financial 
      interest.
EDAT- 2019/04/27 06:00
MHDA- 2019/04/27 06:01
PMCR- 2019/04/30
CRDT- 2019/04/27 06:00
PHST- 2019/01/02 00:00 [received]
PHST- 2019/03/05 00:00 [revised]
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/04/27 06:00 [pubmed]
PHST- 2019/04/27 06:01 [medline]
PHST- 2019/04/27 06:00 [entrez]
PHST- 2019/04/30 00:00 [pmc-release]
AID - ijsc19002 [pii]
AID - ijsc-12-291 [pii]
AID - 10.15283/ijsc19002 [doi]
PST - ppublish
SO  - Int J Stem Cells. 2019 Jul 31;12(2):291-303. doi: 10.15283/ijsc19002.