PMID- 31023230 OWN - NLM STAT- MEDLINE DCOM- 20190517 LR - 20200225 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 19 IP - 1 DP - 2019 Apr 25 TI - Genome constitution and evolution of Elytrigia lolioides inferred from Acc1, EF-G, ITS, TrnL-F sequences and GISH. PG - 158 LID - 10.1186/s12870-019-1779-x [doi] LID - 158 AB - BACKGROUND: Elytrigia lolioides (Kar. et Kir.) Nevski, which is a perennial, cross-pollinating wheatgrass that is distributed in Russia and Kazakhstan, is classified into Elytrigia, Elymus, and Lophopyrum genera by taxonomists on the basis of different taxonomic classification systems. However, the genomic constitution of E. lolioides is still unknown. To identify the genome constitution and evolution of E. lolioides, we used single-copy nuclear genes acetyl-CoA carboxylase (Acc1) and elongation factor G (EF-G), multi-copy nuclear gene internal transcribed space (ITS), chloroplast gene trnL-F together with fluorescence and genomic in situ hybridization. RESULTS: Despite the widespread homogenization of ITS sequences, two distinct lineages (genera Pseudoroegneria and Hordeum) were identified. Acc1 and EF-G sequences suggested that in addition to Pseudoroegneria and Hordeum, unknown genome was the third potential donor of E. lolioides. Data from chloroplast DNA showed that Pseudoroegneria is the maternal donor of E. lolioides. Data from specific FISH marker for St genome indicated that E. lolioides has two sets of St genomes. Both genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) results confirmed the presence of Hordeum genome in this species. When E genome was used as the probe, no signal was found in 42 chromosomes. The E-like copy of Acc1 sequences was detected in E. lolioides possibly due to the introgression from E genome species. One of the H chromosomes in the accession W6-26586 from Kazakhstan did not hybridize H genome signals but had St genome signals on the pericentromeric regions in the two-color GISH. CONCLUSIONS: Phylogenetic and in situ hybridization indicated the presence of two sets of Pseudoroegneria and one set of Hordeum genome in E. lolioides. The genome formula of E. lolioides was designed as StStStStHH. E. lolioides may have originated through the hybridization between tetraploid Elymus (StH) and diploid Pseudoroegneria species. E and unknown genomes may participate in the speciation of E. lolioides through introgression. According to the genome classification system, E. lolioides should be transferred into Elymus L. and renamed as Elymus lolioidus (Kar. er Kir.) Meld. FAU - Wang, Long AU - Wang L AD - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. AD - Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. FAU - Jiang, Yuanyuan AU - Jiang Y AD - College of Science, Sichuan Agricultural University, Ya'an, 625014, Sichuan, China. FAU - Shi, Qinghua AU - Shi Q AD - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing, 100101, China. FAU - Wang, Yi AU - Wang Y AD - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. FAU - Sha, Lina AU - Sha L AD - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. FAU - Fan, Xing AU - Fan X AD - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. FAU - Kang, Houyang AU - Kang H AD - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. FAU - Zhang, Haiqin AU - Zhang H AD - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. FAU - Sun, Genlou AU - Sun G AD - Biology Department, Saint Mary's University, Halifax, Nova Scotia, Canada. FAU - Zhang, Li AU - Zhang L AD - College of Science, Sichuan Agricultural University, Ya'an, 625014, Sichuan, China. FAU - Zhou, Yonghong AU - Zhou Y AD - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. zhouyh@sicau.edu.cn. AD - Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. zhouyh@sicau.edu.cn. LA - eng GR - 31470305/National Natural Science Foundation of China/ GR - 31270243/National Natural Science Foundation of China/ GR - 31200252/National Natural Science Foundation of China/ PT - Journal Article DEP - 20190425 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 RN - 0 (DNA, Ribosomal Spacer) RN - 0 (Peptide Elongation Factor G) RN - EC 6.4.1.2 (Acetyl-CoA Carboxylase) SB - IM MH - Acetyl-CoA Carboxylase/*genetics MH - Base Sequence MH - *Biological Evolution MH - Consensus Sequence MH - DNA, Ribosomal Spacer/*genetics MH - Elymus/*genetics MH - *Genes, Chloroplast MH - *Genome, Plant MH - *In Situ Hybridization, Fluorescence MH - Peptide Elongation Factor G/*genetics MH - Phylogeny PMC - PMC6485066 OTO - NOTNLM OT - Acc1 OT - EF-G OT - Elytrigia lolioides OT - GISH OT - Genome constitution OT - ITS OT - Taxonomy OT - trnL-F COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2019/04/27 06:00 MHDA- 2019/05/18 06:00 PMCR- 2019/04/25 CRDT- 2019/04/27 06:00 PHST- 2018/12/31 00:00 [received] PHST- 2019/04/15 00:00 [accepted] PHST- 2019/04/27 06:00 [entrez] PHST- 2019/04/27 06:00 [pubmed] PHST- 2019/05/18 06:00 [medline] PHST- 2019/04/25 00:00 [pmc-release] AID - 10.1186/s12870-019-1779-x [pii] AID - 1779 [pii] AID - 10.1186/s12870-019-1779-x [doi] PST - epublish SO - BMC Plant Biol. 2019 Apr 25;19(1):158. doi: 10.1186/s12870-019-1779-x.