PMID- 31026586
OWN - NLM
STAT- MEDLINE
DCOM- 20200608
LR  - 20200608
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 137
DP  - 2019 Jun
TI  - Hydrogen sulfide protects against DSS-induced colitis by inhibiting NLRP3 
      inflammasome.
PG  - 99-109
LID - S0891-5849(18)32424-9 [pii]
LID - 10.1016/j.freeradbiomed.2019.04.025 [doi]
AB  - Hydrogen sulfide (H(2)S), as the third gasotransmitter, has been shown to be 
      effective in the prevention of inflammation. In addition, the NLRP3 inflammasome 
      is a key player in the pathogenesis of dextran sulfate sodium (DSS)-induced 
      colitis. Therefore, the aim of our research was to determine whether H(2)S exerts 
      an anti-inflammatory effect on DSS-induced colitis by targeting NLRP3 
      inflammasome. Our data showed that DSS-induced colitis is attenuated by H(2)S, 
      lessening the shortening of the colon lengths and colonic pathological damages. 
      The cytokines TNF-alpha, IL-1beta, and IL-6 in colon samples were also significantly 
      downregulated by H(2)S. Besides, H(2)S markedly suppressed the expression of 
      NLRP3 and cleaved caspase-1 (p20) in colons from DSS-induced colitis mice. More 
      importantly, CSE(-/-) mice were more susceptive to DSS-induced colitis when 
      compared to wild-type (WT) mice. Our experimental results also suggested that 
      H(2)S dose-dependently inhibits the activation of NLRP3 inflammasome in bone 
      marrow-derived macrophages (BMDMs) by reducing the cleavage of caspase-1 and the 
      secretion of IL-1beta. Furthermore, the inhibitory effect of H(2)S is due to a 
      reduction in reactive oxygen species (ROS) generation and partly dependent on the 
      disruption of nuclear erythroid 2-related factor-2 (Nrf2) activation. 
      Collectively, our study confirms that H(2)S exerts its protective effect on 
      DSS-induced mouse colitis at least partly by inhibiting the activation of NLRP3 
      inflammasome pathway.
CI  - Copyright (c) 2019. Published by Elsevier Inc.
FAU - Qin, Ming
AU  - Qin M
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China; State Key 
      Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau 
      University of Science and Technology, Macau, China; Department of Immunology, 
      ZunYi Medical University, Zunyi, 563000, China.
FAU - Long, Fen
AU  - Long F
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China.
FAU - Wu, Weijun
AU  - Wu W
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China.
FAU - Yang, Di
AU  - Yang D
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China.
FAU - Huang, Mengwei
AU  - Huang M
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China.
FAU - Xiao, Chenxi
AU  - Xiao C
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China.
FAU - Chen, Xu
AU  - Chen X
AD  - School of Pharmacy, Guilin Medical University, China.
FAU - Liu, Xinhua
AU  - Liu X
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China. Electronic 
      address: liuxinhua@fudan.edu.cn.
FAU - Zhu, Yi Zhun
AU  - Zhu YZ
AD  - Shanghai Key Laboratory of Bioactive Small Molecules, Department of Pharmacology, 
      School of Pharmacy, Fudan University, Shanghai, 201203, China; State Key 
      Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau 
      University of Science and Technology, Macau, China. Electronic address: 
      yzzhu@must.edu.mo.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190423
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Reactive Oxygen Species)
RN  - 9042-14-2 (Dextran Sulfate)
RN  - EC 3.4.22.36 (Caspase 1)
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/metabolism
MH  - Caspase 1/metabolism
MH  - Colitis/*metabolism
MH  - Colon/*pathology
MH  - Cystathionine gamma-Lyase/genetics/*metabolism
MH  - Dextran Sulfate
MH  - Disease Models, Animal
MH  - Humans
MH  - Hydrogen Sulfide/metabolism
MH  - Inflammasomes/metabolism
MH  - Inflammatory Bowel Diseases/*metabolism
MH  - Macrophages/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Hydrogen sulfide
OT  - NADPH oxidase 4
OT  - NLRP3 inflammasome
OT  - Nuclear factor erythroid 2-related factor 2
OT  - Reactive oxygen species
EDAT- 2019/04/27 06:00
MHDA- 2020/06/09 06:00
CRDT- 2019/04/27 06:00
PHST- 2018/11/17 00:00 [received]
PHST- 2019/04/21 00:00 [revised]
PHST- 2019/04/22 00:00 [accepted]
PHST- 2019/04/27 06:00 [pubmed]
PHST- 2020/06/09 06:00 [medline]
PHST- 2019/04/27 06:00 [entrez]
AID - S0891-5849(18)32424-9 [pii]
AID - 10.1016/j.freeradbiomed.2019.04.025 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2019 Jun;137:99-109. doi: 
      10.1016/j.freeradbiomed.2019.04.025. Epub 2019 Apr 23.