PMID- 31026690
OWN - NLM
STAT- MEDLINE
DCOM- 20190725
LR  - 20190725
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 250
DP  - 2019 Jul
TI  - Expression of resistance genes instead of gene abundance are correlated with 
      trace levels of antibiotics in urban surface waters.
PG  - 437-446
LID - S0269-7491(19)30653-0 [pii]
LID - 10.1016/j.envpol.2019.04.035 [doi]
AB  - In this study, antibiotic resistance to macrolide-lincosamide-streptogramin B 
      (MLS(B)) antibiotics in total microbial community in surface water in a coastal 
      urban city was measured using a modified fluorescence in situ hybridization 
      (FISH) technique. This FISH technique quantified the rate of antibiotic 
      resistance to MLS(B) antibiotics through targeting methylation site of A2058 of 
      23S rRNAs resulting from expressed erythromycin ribosome methylation (erm) genes. 
      Correlations between the rates of MLS(B) resistance measured by FISH and 
      macrolide concentrations was stronger than that between the relative abundance of 
      erm genes and macrolide concentrations, especially in residential areas where the 
      main detected antibiotics were macrolides. These results suggest that trace 
      levels of antibiotics in environmental waters, which was as low as 40 ng L(-1), 
      may still play important roles in the development and spread of antibiotic 
      resistance. Additionally, methylation as a result of erm gene expression, instead 
      of erm gene abundance, was a better indicator of selective pressure of trace 
      level macrolides. The rates of MLS(B) resistance varied significantly among land 
      use types, suggesting that anthropogenic activities are important factors to 
      select for erm gene expression in the environment. Microbial community analysis 
      of representative surface water samples showed that relatively high rates of 
      MLS(B) resistance were observed in Alphaproteobacteria (42%), Acidobacteria 
      (36%), Bacteroidaceae (32%), Chloroflexi (27%), and Betaproteobacteria (20.2%).
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Yi, Xinzhu
AU  - Yi X
AD  - School of Life Sciences, South China Normal University, Guangzhou, Guangdong, 
      510631, China.
FAU - Lin, Chenghui
AU  - Lin C
AD  - Department of Civil and Environmental Engineering, National University of 
      Singapore, Singapore, 117576.
FAU - Ong, Eugene Jie Li
AU  - Ong EJL
AD  - Department of Civil and Environmental Engineering, National University of 
      Singapore, Singapore, 117576.
FAU - Wang, Mian
AU  - Wang M
AD  - Lyles School of Civil Engineering and Division of Environmental and Ecological 
      Engineering, Purdue University, 550 Stadium Mall Drive, West Lafayette, IN, 
      United States.
FAU - Li, Bolin
AU  - Li B
AD  - Wuhan University of Technology, Wuhan, Hubei, 430070, China.
FAU - Zhou, Zhi
AU  - Zhou Z
AD  - Lyles School of Civil Engineering and Division of Environmental and Ecological 
      Engineering, Purdue University, 550 Stadium Mall Drive, West Lafayette, IN, 
      United States. Electronic address: zhizhou@purdue.edu.
LA  - eng
PT  - Journal Article
DEP - 20190410
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Lincosamides)
RN  - 0 (Macrolides)
RN  - 0 (Streptogramin Group B)
RN  - 0 (Water Pollutants, Chemical)
RN  - 11006-76-1 (Virginiamycin)
RN  - 3131-03-1 (Streptogramin B)
RN  - 63937KV33D (Erythromycin)
RN  - J91D5GN5AT (virginiamycin factor S1)
MH  - Anti-Bacterial Agents/*analysis
MH  - Drug Resistance, Microbial/*genetics
MH  - *Environmental Monitoring
MH  - Erythromycin
MH  - Genes, Microbial
MH  - In Situ Hybridization, Fluorescence
MH  - Lincosamides/analysis
MH  - Macrolides/analysis
MH  - Microbial Sensitivity Tests
MH  - Streptogramin B/analysis
MH  - Streptogramin Group B/analysis
MH  - Virginiamycin/analysis
MH  - *Water Microbiology
MH  - Water Pollutants, Chemical/*analysis
OTO - NOTNLM
OT  - Antibiotic resistance
OT  - Fluorescent in situ hybridization
OT  - Macrolide-lincosamide-streptogramin B
OT  - Surface water
EDAT- 2019/04/27 06:00
MHDA- 2019/07/26 06:00
CRDT- 2019/04/27 06:00
PHST- 2019/02/01 00:00 [received]
PHST- 2019/03/30 00:00 [revised]
PHST- 2019/04/06 00:00 [accepted]
PHST- 2019/04/27 06:00 [pubmed]
PHST- 2019/07/26 06:00 [medline]
PHST- 2019/04/27 06:00 [entrez]
AID - S0269-7491(19)30653-0 [pii]
AID - 10.1016/j.envpol.2019.04.035 [doi]
PST - ppublish
SO  - Environ Pollut. 2019 Jul;250:437-446. doi: 10.1016/j.envpol.2019.04.035. Epub 
      2019 Apr 10.