PMID- 31028477
OWN - NLM
STAT- MEDLINE
DCOM- 20200304
LR  - 20200309
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 186
IP  - 5
DP  - 2019 Apr 26
TI  - A carbon dot and gold nanoparticle-based fluorometric immunoassay for 
      8-hydroxy-2'-deoxyguanosine in oxidatively damaged DNA.
PG  - 303
LID - 10.1007/s00604-019-3392-y [doi]
AB  - A method is described for the fluorometric determination of DNA containing 
      oxidatively damaged product 8-hydroxy-2'-deoxyguanosine (DNA-8-OHdG). Carbon dots 
      (CDs) were modified with glutaraldehyde for DNA conjugation, and antibody against 
      8-OHdG was immobilized on gold nanoparticles (AuNPs). The presence of DNA-8-OHdG 
      can be linked to CDs by reaction of amino groups on DNA with glutaraldehyde. 
      AuNPs were brought closely to CDs by specific immune reaction between 8-OHdG and 
      antibody on AuNPs. Under 350 nm photoexcitation, the emission of CDs with a peak 
      at 440 nm is quenched by the AuNPs and not restored. In the presence of 
      DNA-8-OHdG, the measured fluorescence intensity decreases and quenching 
      efficiency increases. The limit of detection is 700 pM, and the assay works in 
      the 0.01 nM to 25 muM DNA-8-OHdG concentration range. The method is perceived to 
      possess a good potential as a tool for detecting biomarkers for DNA damage due to 
      oxidative stress. Graphical abstract A fluorometric immunoassay for detecting 
      8-hydroxy-2'-deoxyguanosine (8-OHdG) in oxidatively damaged DNA is reported. It 
      is based on the use of carbon dots (CDs) and gold nanoparticles (AuNPs). Black 
      wavy lines represent DNA. Yellow polygonal sharps represent 8-OHdG. Blue and pink 
      balls represent CDs and AuNPs, respectively.
FAU - Hu, Wei
AU  - Hu W
AD  - Institute of Ocean Research, Zhejiang University of Technology, Hangzhou, 310014, 
      People's Republic of China.
AD  - Department of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou, 310014, People's Republic of China.
FAU - Chen, Tian
AU  - Chen T
AD  - Institute of Ocean Research, Zhejiang University of Technology, Hangzhou, 310014, 
      People's Republic of China.
AD  - Department of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou, 310014, People's Republic of China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Institute of Ocean Research, Zhejiang University of Technology, Hangzhou, 310014, 
      People's Republic of China.
AD  - Department of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou, 310014, People's Republic of China.
FAU - Ye, Weiwei
AU  - Ye W
AD  - Institute of Ocean Research, Zhejiang University of Technology, Hangzhou, 310014, 
      People's Republic of China. yeweiwei@zjut.edu.cn.
AD  - Department of Food Science and Technology, Zhejiang University of Technology, 
      Hangzhou, 310014, People's Republic of China. yeweiwei@zjut.edu.cn.
LA  - eng
GR  - 81601570/National Natural Science Foundation of China (NSFC)/International
GR  - LQ16C190002/Natural Science Foundation of Zhejiang Province (CN)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190426
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - 0 (Biomarkers)
RN  - 7440-44-0 (Carbon)
RN  - 7440-57-5 (Gold)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine/*analysis/urine
MH  - Biomarkers/analysis/urine
MH  - Carbon/*chemistry
MH  - *DNA Damage
MH  - Fluorometry
MH  - Gold/*chemistry
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Immunoassay/*methods
MH  - Limit of Detection
MH  - Metal Nanoparticles/*chemistry
MH  - Oxidative Stress/*genetics
OTO - NOTNLM
OT  - 8-OHdG
OT  - AuNPs
OT  - CDs
OT  - DNA damage
OT  - fluorometric immunoassay
EDAT- 2019/04/28 06:00
MHDA- 2020/03/05 06:00
CRDT- 2019/04/28 06:00
PHST- 2018/12/07 00:00 [received]
PHST- 2019/03/29 00:00 [accepted]
PHST- 2019/04/28 06:00 [entrez]
PHST- 2019/04/28 06:00 [pubmed]
PHST- 2020/03/05 06:00 [medline]
AID - 10.1007/s00604-019-3392-y [pii]
AID - 10.1007/s00604-019-3392-y [doi]
PST - epublish
SO  - Mikrochim Acta. 2019 Apr 26;186(5):303. doi: 10.1007/s00604-019-3392-y.