PMID- 31030426
OWN - NLM
STAT- MEDLINE
DCOM- 20190815
LR  - 20190815
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 36
IP  - 5
DP  - 2019 May 10
TI  - [Clinical feature and cytogenetic analysis of 28 patients with bone marrow 
      invasion non-Hodgkin's lymphoma].
PG  - 429-432
LID - 10.3760/cma.j.issn.1003-9406.2019.05.004 [doi]
AB  - OBJECTIVE: To study the correlation of hematomorphology, bone marrow cytogenetics 
      and clinical biochemical parameters with the prognosis of non-Hodgkin's lymphoma 
      with bone marrow invasion. METHODS: Morphological analysis of bone marrow cells 
      was performed by routine bone marrow puncture.Chromosome samples were prepared by 
      short-term bone marrow culture. Karyotype analysis was carried out by R-banding 
      in 28 patients. P53 gene was detected by fluorescence in situ hybridization 
      (FISH). Serum lactate dehydrogenase (LDH) of all patients was determined and 
      compared. RESULTS: In all patients, bone marrow morphology showed invasion of 
      lymphoma. Chromosome analysis revealed abnormal karyotypes in 19 cases, which 
      yielded an incidence of 67.85%. The proportion of lymphoma cells in bone marrow 
      among those with an abnormal karyotype was much higher than those with a normal 
      karyotype (60.2% vs. 33.5%, P<0.05). FISH assay showed that 9 (32.14%) patients 
      had P53 gene deletion. And the deletion was much more common among those with an 
      abnormal karyotype (42.11% vs. 11.11%, P<0.05). The serum LDH level in patients 
      with an abnormal karyotype was significantly higher compared with whose with a 
      normal karyotype (1464.37 U/L vs. 294.33 U/L, P<0.05). CONCLUSION: Patients with 
      abnormal karyotypes have a higher rate of P53 gene deletion, and their LDH level 
      is significantly higher than those with a normal karyotype, which predicted a 
      relatively poor prognosis.
FAU - Cen, Ling
AU  - Cen L
AD  - Central Laboratory, Changzhou Second People's Hospital Affiliated to Nanjing 
      Medical University, Changzhou, Jiangsu 213003, China. Email: 15295189493@163.com.
FAU - Jiang, Yu
AU  - Jiang Y
FAU - Chen, Tao
AU  - Chen T
FAU - Chao, Hongying
AU  - Chao H
FAU - Lu, Xuzhang
AU  - Lu X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
MH  - Adult
MH  - *Bone Marrow
MH  - Child
MH  - Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Lymphoma, Non-Hodgkin
EDAT- 2019/04/29 06:00
MHDA- 2019/08/16 06:00
CRDT- 2019/04/29 06:00
PHST- 2019/04/29 06:00 [entrez]
PHST- 2019/04/29 06:00 [pubmed]
PHST- 2019/08/16 06:00 [medline]
AID - 940636082 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2019.05.004 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2019 May 10;36(5):429-432. doi: 
      10.3760/cma.j.issn.1003-9406.2019.05.004.