PMID- 31031544
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231011
IS  - 0931-1785 (Print)
IS  - 1439-0434 (Electronic)
IS  - 0931-1785 (Linking)
VI  - 166
IP  - 10
DP  - 2018 Oct
TI  - Efficacy of chemotherapy and thermotherapy in elimination of East African cassava 
      mosaic virus from Tanzanian cassava landrace.
PG  - 739-745
LID - 10.1111/jph.12725 [doi]
AB  - Cassava mosaic disease is caused by cassava mosaic begomoviruses (CMBs) and can 
      result in crop losses up to 100% in cassava (Manihot esculenta) in Tanzania. We 
      investigated the efficacy of chemotherapy and thermotherapy for elimination of 
      East African cassava mosaic virus (EACMV) of Tanzanian cassava. In vitro 
      plantlets from EACMV-infected plants obtained from coastal Tanzania were 
      established in the greenhouse. Leaves were sampled from the plants and tested to 
      confirm the presence of EACMV. Plantlets of plants positive for EACMV were 
      initiated in Murashige and Skoog (MS) medium. On the second subculture, they were 
      subjected into chemical treatment in the medium containing salicylic acid (0, 10, 
      20, 30 and 40 mg/L) and ribavirin (0, 5, 10, 15 and 20 mg/L). In the second 
      experiment, EACMV-infected plantlets were subjected to temperatures between 35 
      and 40 degrees C with 28 degrees C as the control. After 42 days of growth, DNA was extracted 
      from plant leaves and PCR amplification was performed using EACMV specific 
      primers. It was found that plant survival decreased with increasing levels of 
      both salicylic acid and ribavirin concentrations. In general, plants treated with 
      salicylic acid exhibited a lower plant survival % than those treated with 
      ribavirin. However, the percentage of virus-free plants increased with an 
      increase in the concentration of both ribavirin and salicylic acid. The most 
      effective concentrations were 20 mg/L of ribavirin and 30 mg/L of salicylic acid; 
      these resulted in 85.0% and 88.9% virus-free plantlets, respectively. With regard 
      to thermotherapy, 35 degrees C resulted in 79.5% virus-free plantlets compared to 69.5% 
      at 40 degrees C. Based on virus elimination, ribavirin at 20 mg/L, salicylic acid 30 mg/L 
      and thermotherapy at 35 degrees C are recommended for production of EACMV free cassava 
      plantlets from infected cassava landraces.
FAU - Kidulile, Christina Edward
AU  - Kidulile CE
AUID- ORCID: 0000-0002-2719-519X
AD  - Department of Biotechnology Mikocheni Agricultural Research Institute Dar es 
      Salaam Tanzania.
AD  - Institute for Biotechnology Research Jomo Kenyatta University of Agriculture and 
      Technology Nairobi Kenya.
FAU - Miinda Ateka, Elijah
AU  - Miinda Ateka E
AD  - Department of Horticulture Jomo Kenyatta University of Agriculture and Technology 
      Nairobi Kenya.
FAU - Alakonya, Amos Emitati
AU  - Alakonya AE
AD  - Institute for Biotechnology Research Jomo Kenyatta University of Agriculture and 
      Technology Nairobi Kenya.
AD  - International Institute of Tropical Agriculture Ibadan Nigeria.
FAU - Ndunguru, Joseph Canisius
AU  - Ndunguru JC
AD  - Department of Biotechnology Mikocheni Agricultural Research Institute Dar es 
      Salaam Tanzania.
LA  - eng
PT  - Journal Article
DEP - 20180812
PL  - Germany
TA  - J Phytopathol (1986)
JT  - Journal of phytopathology (1986)
JID - 101639096
PMC - PMC6473628
OTO - NOTNLM
OT  - EACMV
OT  - cassava mosaic begomoviruses
OT  - ribavirin
OT  - salicylic acid
OT  - virus-free plantlets
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be constructed as potential 
      conflict of interest.
EDAT- 2019/04/30 06:00
MHDA- 2019/04/30 06:01
PMCR- 2019/04/19
CRDT- 2019/04/30 06:00
PHST- 2017/05/29 00:00 [received]
PHST- 2018/03/02 00:00 [revised]
PHST- 2018/06/02 00:00 [accepted]
PHST- 2019/04/30 06:00 [entrez]
PHST- 2019/04/30 06:00 [pubmed]
PHST- 2019/04/30 06:01 [medline]
PHST- 2019/04/19 00:00 [pmc-release]
AID - JPH12725 [pii]
AID - 10.1111/jph.12725 [doi]
PST - ppublish
SO  - J Phytopathol (1986). 2018 Oct;166(10):739-745. doi: 10.1111/jph.12725. Epub 2018 
      Aug 12.