PMID- 31037357 OWN - NLM STAT- MEDLINE DCOM- 20200106 LR - 20200106 IS - 1432-0878 (Electronic) IS - 0302-766X (Linking) VI - 378 IP - 1 DP - 2019 Oct TI - Mouse model to study pulmonary intravascular macrophage recruitment and lung inflammation in acute necrotizing pancreatitis. PG - 97-111 LID - 10.1007/s00441-019-03023-9 [doi] AB - Patients suffering from severe acute pancreatitis (AP) can develop acute lung injury (ALI) with poor outcomes and the mechanisms involved remain incompletely understood. Pulmonary intravascular macrophages (PIMs), which are credited as promoters of ALI, are not constitutively present in humans and rodents; however, there is evidence of PIM recruitment in rodents during some pathological conditions, such as hepatic diseases. Therefore, this study assesses PIM recruitment in the lungs of a mouse model of acute necrotizing pancreatitis (ANP) induced with L-arginine monohydrochloride. Mice were euthanized after 24 h, 72 h and 120 h. Control mice received sham injections of saline. Pancreatic histopathological grading and plasma amylase were used to confirm the development of ANP in L-arginine-treated mice. Histopathological grading of lungs from the ANP mice at 72 h showed increased mononuclear phagocytes in alveolar septa, compared to that from the controls. Lungs from the ANP mice also showed increased numbers of CD68-immunopositive alveolar septal macrophages, suggestive of PIM recruitment, compared to those from the controls. Lungs from the ANP mice showed increased expression of IL-6, IL-10, monocyte chemoattractant protein 1 (MCP-1) and von Willebrand factor compared to those from the controls. The recruitment of CD68-positive septal macrophages was not observed in MCP-1 knockout mice with ANP at 72 h when compared to C57BL/6 wild-type mice. Taken together, we developed a mouse model of PIM recruitment dependent on MCP-1 that allows us to explore their roles in ANP-associated ALI. FAU - Vrolyk, Vanessa AU - Vrolyk V AD - Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada. FAU - Schneberger, David AU - Schneberger D AD - Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada. FAU - Le, Khanh AU - Le K AD - Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada. FAU - Wobeser, Bruce K AU - Wobeser BK AD - Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada. FAU - Singh, Baljit AU - Singh B AD - Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada. baljit.singh1@ucalgary.ca. AD - Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, T2N 4Z6, Canada. baljit.singh1@ucalgary.ca. LA - eng GR - 2014-04308/RGPIN/ PT - Journal Article DEP - 20190429 PL - Germany TA - Cell Tissue Res JT - Cell and tissue research JID - 0417625 SB - IM MH - Animals MH - Disease Models, Animal MH - *Macrophage Activation MH - *Macrophages, Alveolar/metabolism/pathology MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Pancreatitis, Acute Necrotizing/*immunology/pathology MH - Pneumonia/*immunology/pathology OTO - NOTNLM OT - CD68 OT - Mouse OT - Pancreatitis OT - Pulmonary intravascular macrophage OT - vWF EDAT- 2019/05/01 06:00 MHDA- 2020/01/07 06:00 CRDT- 2019/05/01 06:00 PHST- 2018/08/21 00:00 [received] PHST- 2019/03/27 00:00 [accepted] PHST- 2019/05/01 06:00 [pubmed] PHST- 2020/01/07 06:00 [medline] PHST- 2019/05/01 06:00 [entrez] AID - 10.1007/s00441-019-03023-9 [pii] AID - 10.1007/s00441-019-03023-9 [doi] PST - ppublish SO - Cell Tissue Res. 2019 Oct;378(1):97-111. doi: 10.1007/s00441-019-03023-9. Epub 2019 Apr 29.