PMID- 31039523
OWN - NLM
STAT- MEDLINE
DCOM- 20190724
LR  - 20190724
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 212
DP  - 2019 Jul
TI  - The toxic mechanisms of BDE-47 to the marine diatom Thalassiosira pseudonana-a 
      study based on multiple physiological processes.
PG  - 20-27
LID - S0166-445X(19)30020-7 [pii]
LID - 10.1016/j.aquatox.2019.04.010 [doi]
AB  - Polybrominated diphenyl ethers (PBDEs), a series of highly persistent organic 
      pollutants (POPs), are ubiquitous in marine ecosystems. As key primary producers, 
      microalgae are of great importance on evaluating the environmental outcome of 
      PBDEs pollution. In this study, the toxic mechanisms of BDE-47 on the marine 
      diatom Thalassiosira pseudonana were evaluated by measuring multiple 
      physiological processes. Three concentrations of BDE-47 (25, 15 and 5 mug L(-1)) 
      were used along with two controls (blank: no BDE-47 or DMSO; negative control: 
      only DMSO). Experiments lasted 144 h (6 days), in which the actual BDE-47 
      concentrations, cell densities, nutrient (nitrate and phosphate) uptake, pigment 
      compositions, photosynthetic physiology, cell morphology and cellular contents 
      (organic carbon and nitrogen) were measured at 12-48 h intervals. The toxic 
      mechanisms of BDE-47 on T. pseudonana cells were evaluated by measuring multiple 
      physiological processes including photosynthesis, nutrient uptake, cellular 
      material synthesis and cell cycle progressions. The cell divisions of T. 
      pseudonana were severely inhibited by the stress of BDE-47, but the 
      photosynthetic parameters were much less declined and recovered earlier than the 
      cell divisions in the same BDE-47 treatments. The unsuppressed uptake rates of 
      nutrients, increased cell volume and cellular contents indicated the cellular 
      material synthesis proceeded normally. Finally, we found that the cell cycle was 
      arrested in G2/M phase under the stress of BDE-47, we thus concluded that the 
      inhibition of cell divisions by BDE-47 was not due to the lack of energy or 
      cellular materials, where the cell cycle arrest happened; this might be the most 
      important toxicological outcome.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Zhao, Yirong
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Tang, Xuexi
AU  - Tang X
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China.
FAU - Quigg, Antonietta
AU  - Quigg A
AD  - Department of Marine Biology, Texas A&M University, Galveston, Texas, 77553, USA; 
      Department of Oceanography, Texas A&M University, College Station, Texas, 77843, 
      USA.
FAU - Lv, Mengchen
AU  - Lv M
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China. Electronic address: zhaoyan@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190416
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Photosystem II Protein Complex)
RN  - 0 (Pigments, Biological)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0N97R5X10X (2,2',4,4'-tetrabromodiphenyl ether)
RN  - 7440-44-0 (Carbon)
RN  - N762921K75 (Nitrogen)
MH  - Carbon/metabolism
MH  - Cell Cycle/drug effects
MH  - Diatoms/cytology/*drug effects/growth & development/*physiology
MH  - Halogenated Diphenyl Ethers/*toxicity
MH  - Nitrogen/metabolism
MH  - Photosynthesis/drug effects
MH  - Photosystem II Protein Complex/metabolism
MH  - Physiological Phenomena/*drug effects
MH  - Pigments, Biological/metabolism
MH  - Stress, Physiological/drug effects
MH  - Water Pollutants, Chemical/*toxicity
OTO - NOTNLM
OT  - BDE-47
OT  - Cell cycle arrest
OT  - Cellular materials
OT  - PBDEs
OT  - POPs, Thalassiosira pseudonana
OT  - Photosynthesis
OT  - Population growth
EDAT- 2019/05/01 06:00
MHDA- 2019/07/25 06:00
CRDT- 2019/05/01 06:00
PHST- 2019/01/07 00:00 [received]
PHST- 2019/04/13 00:00 [revised]
PHST- 2019/04/15 00:00 [accepted]
PHST- 2019/05/01 06:00 [pubmed]
PHST- 2019/07/25 06:00 [medline]
PHST- 2019/05/01 06:00 [entrez]
AID - S0166-445X(19)30020-7 [pii]
AID - 10.1016/j.aquatox.2019.04.010 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2019 Jul;212:20-27. doi: 10.1016/j.aquatox.2019.04.010. Epub 2019 
      Apr 16.