PMID- 3103991
OWN - NLM
STAT- MEDLINE
DCOM- 19870505
LR  - 20180216
IS  - 0009-3157 (Print)
IS  - 0009-3157 (Linking)
VI  - 33
IP  - 1
DP  - 1987
TI  - A rapid method for the evaluation of new antituberculous agents.
PG  - 22-7
AB  - Fifty-one new synthetic compounds belonging to four different series, namely (1a) 
      substituted aryl-4-(substituted phenyl) succinimide; (1b) N-(substituted 
      methyl)-4-(heterocyclic) succinimide; (2) heterocyclic 4-(5'-nitro-2-furyl) 
      thiazoles; (3) substituted aryl-4-(3', 4'-dihydroxy phenyl) thiazoles, and (4) 
      phenyl-N,N-1,2,3-bis-methoxy carbonyl guanidines were screened for 
      antituberculous activity using a conventional broth dilution test (BDT) and a 
      liquid scintillation radiometric method (LSRM). Eight compounds showed in vitro 
      activity. LSRM showed 100% agreement with BDT. LSRM is completed within 60 h, 
      while BDT requires 8-9 days. Unlike BDT, LSRM allowed one to measure the graded 
      changes in the metabolism and the growth rate of Mycobacterium tuberculosis in 
      response to various concentrations of the drug. It permits the measurement of the 
      relative prolongation of the replication time by the drug or the test compound. 
      With LSRM it was possible to detect the phase of growth during which the test 
      compound shows or begins its antituberculous activity. It improves our 
      understanding of the antituberculous activity of the test compound and hence is 
      more advantageous. It is rapid, reliable, quantitative and more sensitive than 
      BDT. LSRM is thus suitable for the evaluation of new drugs.
FAU - Ashtekar, D R
AU  - Ashtekar DR
FAU - Fernandes, F
AU  - Fernandes F
FAU - Khadse, B G
AU  - Khadse BG
FAU - Shirodkar, M V
AU  - Shirodkar MV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Chemotherapy
JT  - Chemotherapy
JID - 0144731
RN  - 0 (Antitubercular Agents)
RN  - 0 (Guanidines)
RN  - 0 (Succinimides)
RN  - 0 (Thiazoles)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 2KNI5N06TI (Pyrazinamide)
RN  - 8G167061QZ (Ethambutol)
RN  - V83O1VOZ8L (Isoniazid)
RN  - VJT6J7R4TR (Rifampin)
RN  - Y45QSO73OB (Streptomycin)
SB  - IM
MH  - Antitubercular Agents/*pharmacology
MH  - Carbon Dioxide/metabolism
MH  - Ethambutol/pharmacology
MH  - Guanidines/pharmacology
MH  - Isoniazid/pharmacology
MH  - Mycobacterium tuberculosis/*drug effects/growth & development/metabolism
MH  - Pyrazinamide/pharmacology
MH  - Rifampin/pharmacology
MH  - *Scintillation Counting/methods
MH  - Streptomycin/pharmacology
MH  - Succinimides/pharmacology
MH  - Thiazoles/pharmacology
MH  - Time Factors
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1159/000238472 [doi]
PST - ppublish
SO  - Chemotherapy. 1987;33(1):22-7. doi: 10.1159/000238472.