PMID- 31039968
OWN - NLM
STAT- MEDLINE
DCOM- 20190916
LR  - 20190916
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 30
IP  - 7
DP  - 2018 Jun
TI  - D-Aspartate amends reproductive performance of aged roosters by changing gene 
      expression and testicular histology.
PG  - 1038-1048
LID - 10.1071/RD17072 [doi]
AB  - Male broiler breeders (n=32) of 55 weeks of age were administered four different 
      doses of capsulated d-aspartate (DA; 0, 100, 200 or 300mgkg-1day-1, p.o. (DA0, 
      DA100, DA200 and DA300 respectively)) for 12 successive weeks to assess 
      reproductive performance, blood testosterone, testicular histology and transcript 
      levels of steroidogenic acute regulatory protein (StAR), cholesterol side-chain 
      cleavage enzyme (P450scc), androgen receptor (AR), LH receptor (LHR), 
      3beta-hydroxysteroid dehydrogenase (3BHSD), proliferating cell nuclear antigen 
      (PCNA), glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) and glutamate 
      ionotropic receptor NMDA type subunit 2B (GRIN2B). Blood samples and ejaculates 
      were collected, and bodyweight was recorded weekly for 10 weeks. AI was performed 
      weekly for the last 2 weeks to determine the number of sperm penetration holes in 
      the perivitelline layer, fertility and hatchability. Testes histology and 
      transcript levels were evaluated in the 12th week. Bodyweight, numbers of Leydig 
      cells and blood vessels, testis index and levels of sperm abnormalities were not 
      affected (P>0.05) by the treatment. However, sperm total and forward motility, 
      plasma membrane integrity and functionality of sperm, ejaculate volume, 
      testosterone concentration and fertility were higher (P<0.05) in both the DA200 
      and DA300 groups compared with the other groups. In the DA100 and DA200 groups, 
      sperm concentration, number of spermatogonia, thickness of the seminiferous 
      epithelium and the diameter of tubules were significantly higher (P<0.05) than 
      the other DA-treated groups. The number of penetration holes, hatchability and 
      malondialdehyde concentration were higher in the DA200, all DA-treated and DA300 
      groups respectively compared with the control and other treatment groups. Except 
      for P450scc, AR, LHR and PCNA transcript levels in the DA300 groups, the relative 
      expression of the genes evaluated improved significantly in the other DA-treated 
      groups. Based on these experimental findings, it is concluded that DA improves 
      reproductive performance of aged roosters.
FAU - Ansari, Mahdi
AU  - Ansari M
AD  - Department of Animal Science, College of Agriculture and Natural Resources, 
      University of Tehran, Karaj 31587-77871, Iran.
FAU - Zhandi, Mahdi
AU  - Zhandi M
AD  - Department of Animal Science, College of Agriculture and Natural Resources, 
      University of Tehran, Karaj 31587-77871, Iran.
FAU - Kohram, Hamid
AU  - Kohram H
AD  - Department of Animal Science, College of Agriculture and Natural Resources, 
      University of Tehran, Karaj 31587-77871, Iran.
FAU - Zaghari, Mojtaba
AU  - Zaghari M
AD  - Department of Animal Science, College of Agriculture and Natural Resources, 
      University of Tehran, Karaj 31587-77871, Iran.
FAU - Sadeghi, Mostafa
AU  - Sadeghi M
AD  - Department of Animal Science, College of Agriculture and Natural Resources, 
      University of Tehran, Karaj 31587-77871, Iran.
FAU - Gholami, Maedeh
AU  - Gholami M
AD  - Department of Animal Science, College of Animal Science and Fisheries, Sari 
      Agricultural Science Natural Resources University, Sari 4818168984, Iran.
FAU - Deldar, Hamid
AU  - Deldar H
AD  - Department of Animal Science, College of Animal Science and Fisheries, Sari 
      Agricultural Science Natural Resources University, Sari 4818168984, Iran.
FAU - Di Fiore, Maria Maddalena
AU  - Di Fiore MM
AD  - Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, 
      Universita degli Studi della Campania Luigi Vanvitelli, via Vivaldi, 43, 81100 
      Caserta, Italy.
FAU - Benson, Andrew Parks
AU  - Benson AP
AD  - Department of Poultry Science, University of Georgia, Athens, GA 30602-2772, USA.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Drosophila Proteins)
RN  - 0 (Nmdar1 protein, Drosophila)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
RN  - EC 1.14.15.6 (Cholesterol Side-Chain Cleavage Enzyme)
SB  - IM
MH  - Animals
MH  - Chickens/physiology
MH  - Cholesterol Side-Chain Cleavage Enzyme
MH  - D-Aspartic Acid/*pharmacology
MH  - Drosophila Proteins/metabolism
MH  - Gene Expression/*drug effects
MH  - Male
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Receptors, Androgen/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Reproduction/*drug effects/physiology
MH  - Semen Analysis
MH  - Spermatogenesis/*drug effects
MH  - Testis/*drug effects/metabolism
EDAT- 2019/05/02 06:00
MHDA- 2019/09/17 06:00
CRDT- 2019/05/02 06:00
PHST- 2017/02/26 00:00 [received]
PHST- 2017/11/28 00:00 [accepted]
PHST- 2019/05/02 06:00 [entrez]
PHST- 2019/05/02 06:00 [pubmed]
PHST- 2019/09/17 06:00 [medline]
AID - RD17072 [pii]
AID - 10.1071/RD17072 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2018 Jun;30(7):1038-1048. doi: 10.1071/RD17072.