PMID- 31040221 OWN - NLM STAT- MEDLINE DCOM- 20200713 LR - 20200713 IS - 2042-7670 (Electronic) IS - 0042-4900 (Linking) VI - 185 IP - 1 DP - 2019 Jul 6 TI - Porcine reproductive and respiratory syndrome virus RNA detection in different matrices under typical storage conditions in the UK. PG - 21 LID - 10.1136/vr.105312 [doi] AB - In the UK, approximately 40 per cent of the pig breeding herds are outdoors. To monitor their porcine reproductive and respiratory syndrome virus (PRRSV) status, blood is collected commonly from piglets around weaning. Sample collection in British outdoor pigs often occurs during the early morning hours when the piglets tend to accumulate inside sheltered areas. For practical reasons, dry cotton swabs are occasionally used for blood collection and stored at room temperature until arrival in the laboratory. Detection of PRRSV RNA is a function of viral concentration, sample type and storage condition. To evaluate a possible impact of the sampling protocol on PRRSV1 detection, experimentally spiked blood samples using three dilutions of a representative PRRSV1 strain were prepared. In addition, blood samples from pigs naturally infected with PRRSV were obtained from a PRRSV-positive British herd. Spiked blood and blood from infected pigs were used to obtain sera, dry or wet (immersed in saline) polyester or cotton swabs and FTA cards. The different samples were stored for 24 hours, 48 hours or 7 days at 4 degrees C or 20 degrees C and tested by a real-time reverse transcriptase PRRSV PCR assay. Under the study conditions, the best matrix was serum (96.7 per cent), followed by wet swabs (78 per cent), dry swabs (61.3 per cent) and FTA cards (51 per cent). Polyester swabs (76 per cent) showed a better performance than cotton swabs (63.3 per cent). The reduction in sensitivity obtained for swabs and FTA cards was particularly high at low viral concentrations. The results indicate that wet polyester swabs should be used whenever possible. CI - (c) British Veterinary Association 2019. No commercial re-use. See rights and permissions. Published by BMJ. FAU - Fan, Jinghui AU - Fan J AD - The Roslin Institute, University of Edinburgh, Midlothian, UK. AD - College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, China. FAU - Gerber, Priscilla F AU - Gerber PF AD - Animal Science, School of Environmental and Rural Science, University of New England, Armidale, Australia. FAU - Cubas Atienzar, Ana AU - Cubas Atienzar A AD - The Roslin Institute, University of Edinburgh, Midlothian, UK. FAU - Eppink, Lysan AU - Eppink L AD - The Roslin Institute, University of Edinburgh, Midlothian, UK. FAU - Wang, Chong AU - Wang C AD - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, USA. FAU - Opriessnig, Tanja AU - Opriessnig T AUID- ORCID: 0000-0001-9642-0904 AD - The Roslin Institute, University of Edinburgh, Midlothian, UK. AD - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, USA. LA - eng PT - Journal Article DEP - 20190430 PL - England TA - Vet Rec JT - The Veterinary record JID - 0031164 RN - 0 (RNA, Viral) SB - IM MH - Animals MH - Polymerase Chain Reaction/methods/*veterinary MH - Porcine Reproductive and Respiratory Syndrome/*diagnosis MH - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & purification MH - RNA, Viral/*analysis MH - Scotland MH - Specimen Handling/methods/*veterinary MH - Swine MH - Swine Diseases/*diagnosis MH - United Kingdom OTO - NOTNLM OT - diagnostics OT - infectious diseases OT - pcr OT - pigs COIS- Competing interests: None declared. EDAT- 2019/05/02 06:00 MHDA- 2020/07/14 06:00 CRDT- 2019/05/02 06:00 PHST- 2018/12/06 00:00 [received] PHST- 2019/03/29 00:00 [revised] PHST- 2019/04/07 00:00 [accepted] PHST- 2019/05/02 06:00 [pubmed] PHST- 2020/07/14 06:00 [medline] PHST- 2019/05/02 06:00 [entrez] AID - vr.105312 [pii] AID - 10.1136/vr.105312 [doi] PST - ppublish SO - Vet Rec. 2019 Jul 6;185(1):21. doi: 10.1136/vr.105312. Epub 2019 Apr 30.