PMID- 31042461
OWN - NLM
STAT- MEDLINE
DCOM- 20200706
LR  - 20200908
IS  - 2211-1247 (Electronic)
VI  - 27
IP  - 5
DP  - 2019 Apr 30
TI  - LOVIT Is a Putative Vesicular Histamine Transporter Required in Drosophila for 
      Vision.
PG  - 1327-1333.e3
LID - S2211-1247(19)30489-9 [pii]
LID - 10.1016/j.celrep.2019.04.024 [doi]
AB  - Classical fast neurotransmitters are loaded into synaptic vesicles and 
      concentrated by the action of a specific vesicular transporter before being 
      released from the presynaptic neuron. In Drosophila, histamine is distributed 
      mainly in photoreceptors, where it serves as the main neurotransmitter for visual 
      input. In a targeted RNAi screen for neurotransmitter transporters involved in 
      concentrating photoreceptor synaptic histamine, we identified an SLC45 
      transporter protein, LOVIT (loss of visual transmission). LOVIT is prominently 
      expressed in photoreceptor synaptic vesicles and is required for Drosophila 
      visual neurotransmission. Null mutations of lovit severely reduced the 
      concentration of histamine in photoreceptor terminals. These results demonstrate 
      a LOVIT-dependent mechanism, maintaining the synaptic concentration of histamine, 
      and provide evidence for a histamine vesicular transporter besides the vesicular 
      monoamine transporter (VMAT) family.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Xu, Ying
AU  - Xu Y
AD  - National Institute of Biological Sciences, Beijing, China; Department of Surgery, 
      University of Michigan, Ann Arbor, MI, USA.
FAU - Wang, Tao
AU  - Wang T
AD  - National Institute of Biological Sciences, Beijing, China; Tsinghua Institute of 
      Multidisciplinary Biomedical Research, Tsinghua University, Beijing, China. 
      Electronic address: wangtao1006@nibs.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Drosophila Proteins)
RN  - 0 (Symporters)
RN  - 0 (lovit protein, Drosophila)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Drosophila Proteins/genetics/*physiology
MH  - Drosophila melanogaster/genetics/*physiology
MH  - Histamine/metabolism
MH  - Loss of Function Mutation
MH  - Photoreceptor Cells, Invertebrate/*physiology
MH  - Presynaptic Terminals/metabolism
MH  - Symporters/genetics/*physiology
MH  - Synaptic Vesicles/metabolism
MH  - *Vision, Ocular
OTO - NOTNLM
OT  - histamine
OT  - neurotransmitter concentration
OT  - synaptic transmission
OT  - vesicular transporter
EDAT- 2019/05/03 06:00
MHDA- 2020/07/07 06:00
CRDT- 2019/05/03 06:00
PHST- 2018/11/29 00:00 [received]
PHST- 2019/02/17 00:00 [revised]
PHST- 2019/04/02 00:00 [accepted]
PHST- 2019/05/03 06:00 [entrez]
PHST- 2019/05/03 06:00 [pubmed]
PHST- 2020/07/07 06:00 [medline]
AID - S2211-1247(19)30489-9 [pii]
AID - 10.1016/j.celrep.2019.04.024 [doi]
PST - ppublish
SO  - Cell Rep. 2019 Apr 30;27(5):1327-1333.e3. doi: 10.1016/j.celrep.2019.04.024.