PMID- 31043276
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2213-2945 (Print)
IS  - 2213-2953 (Linking)
VI  - 7
IP  - 4
DP  - 2018 Jul-Aug
TI  - Fluorescence in situ hybridization of thyroid fine-needle aspiration biopsy 
      distinguishes between neoplastic and non-neoplastic Hurthle cell lesions.
PG  - 195-200
LID - S2213-2945(18)30040-1 [pii]
LID - 10.1016/j.jasc.2018.04.004 [doi]
AB  - INTRODUCTION: Fine-needle aspiration (FNA) biopsy of Hurthle cell proliferations 
      can be difficult to characterize based purely on morphologic features. Studies 
      have shown Hurthle cell neoplasms often demonstrate gains in chromosomes 5, 7, 
      and 12. This study examined fluorescence in situ hybridization (FISH) performance 
      characteristics in non-neoplastic and neoplastic Hurthle cell proliferations 
      sampled by FNA biopsy in order to assess chromosome patterns. MATERIALS AND 
      METHODS: FNA biopsies of Hurthle cell proliferations, including nodular 
      hyperplasia (NH), Hurthle cell adenoma (HCA), and Hurthle cell carcinoma (HCC), 
      that had subsequent surgical excision were selected. FISH was performed on an 
      air-dried, modified Wright-Giemsa-stained, aspirate smear slide from each case 
      using a 3-color panel consisting of 1 subtelomeric and 2 centromeric probes for 
      chromosomes 5, 7, and 12. Chromosomal probe patterns were recorded in up to 50 
      cells. A positive result was considered when >15% of cells showed a polysomy in 2 
      or more chromosomes. RESULTS: A total of 25 cases were included in the study. All 
      cases of NH were negative, and 7 of 9 (78%) HCAs and 8 of 12 (67%) HCCs were 
      positive. Of the positive cases, 2 of the 7 (29%) HCAs showed >50% of cells with 
      polysomy, and 5 of the 8 (63%) HCCs showed >50% of the cells with polysomy. 
      CONCLUSION: Thyroid FNA biopsy can identify Hurthle cell proliferations; risk 
      stratification based on morphology is difficult, however. FISH chromosomal 
      evaluation of thyroid FNA biopsies is useful to distinguish neoplastic from 
      non-neoplastic Hurthle cell proliferation.
CI  - Copyright (c) 2018 American Society of Cytopathology. Published by Elsevier Inc. 
      All rights reserved.
FAU - Bibbey, Scott M
AU  - Bibbey SM
AD  - Department of Pathology and Immunology, Washington University School of Medicine, 
      St. Louis, Missouri.
FAU - Collins, Brian T
AU  - Collins BT
AD  - Department of Pathology and Immunology, Washington University School of Medicine, 
      St. Louis, Missouri.
FAU - Bernadt, Cory T
AU  - Bernadt CT
AD  - Department of Pathology and Immunology, Washington University School of Medicine, 
      St. Louis, Missouri. Electronic address: cbernadt@wustl.edu.
LA  - eng
PT  - Journal Article
DEP - 20180427
PL  - United States
TA  - J Am Soc Cytopathol
JT  - Journal of the American Society of Cytopathology
JID - 101613234
OTO - NOTNLM
OT  - Fine-needle aspiration
OT  - Fluorescence in-situ hybridization
OT  - Hurthle cell
OT  - Thyroid
EDAT- 2018/01/01 00:00
MHDA- 2018/01/01 00:01
CRDT- 2019/05/03 06:00
PHST- 2018/03/09 00:00 [received]
PHST- 2018/04/18 00:00 [revised]
PHST- 2018/04/23 00:00 [accepted]
PHST- 2019/05/03 06:00 [entrez]
PHST- 2018/01/01 00:00 [pubmed]
PHST- 2018/01/01 00:01 [medline]
AID - S2213-2945(18)30040-1 [pii]
AID - 10.1016/j.jasc.2018.04.004 [doi]
PST - ppublish
SO  - J Am Soc Cytopathol. 2018 Jul-Aug;7(4):195-200. doi: 10.1016/j.jasc.2018.04.004. 
      Epub 2018 Apr 27.