PMID- 31043294
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2213-2945 (Print)
IS  - 2213-2953 (Linking)
VI  - 6
IP  - 6
DP  - 2017 Nov-Dec
TI  - Pneumocystis jirovecii immunostain versus Gomori/Grocott methenamine silver stain 
      of bronchoalveolar lavage in cell blocks: an institutional experience.
PG  - 242-247
LID - S2213-2945(17)30072-8 [pii]
LID - 10.1016/j.jasc.2017.06.207 [doi]
AB  - INTRODUCTION: Current approaches to Pneumocystis jirovecii (PCJ) screening on 
      bronchioalveolar lavage samples (BAL) include Gomori/Grocott methenamine silver 
      stain (GMS), toluidine blue O stain, Wright-Giemsa stain, immunofluorescent 
      antibody stain, and polymerase chain reaction. Another method available is PCJ 
      immunohistochemistry stain (PCJ IHC). There are no published series evaluating 
      the efficacy of PCJ IHC in cell block preparation of BAL, we sought to compare 
      GMS versus PCJ IHC at our institution. MATERIALS AND METHODS: We performed a 
      retrospective analysis at our institution of all BAL with cell blocks where PCJ 
      IHC and GMS were done simultaneously since March 2015. RESULTS: 982 BAL samples 
      were identified from 640 patients (median age: 54 years; range: 1-84 years). For 
      895 cases, GMS and PCJ IHC were performed simultaneously. PCJ was identified in 
      14 samples, from 13 patients (2.2% of patients) using PCJ IHC. GMS stains were 
      read as positive in only 6 of these 14 cases (42.8%); in two of those cases, PCJ 
      was easily identified on routine Papanicolaou stains. We repeated GMS staining on 
      those 14 cases following before-schedule maintenance in our Ventana Benchmark 
      Autostainer, identifying 12 cases positive. In addition, a significantly higher 
      number of organisms was seen on repeat GMS (median: 58) than the original GMS 
      (median: 8.7). Nevertheless, a statistically significant higher number of 
      organisms was detected by PCJ IHC (median: 474). CONCLUSIONS: PCJ IHC performed 
      in cell block is more sensitive and specific than GMS and is a reliable marker 
      when a low number of PCJ organisms are present.
CI  - Copyright (c) 2017 American Society of Cytopathology. Published by Elsevier Inc. 
      All rights reserved.
FAU - Gonzalez, Abel Arnoldo
AU  - Gonzalez AA
AD  - Department of Clinical Pathology & Cell Biology, Columbia University Medical 
      Center, New York Presbyterian Hospital, New York, New York. Electronic address: 
      aag2161@columbia.edu.
FAU - Hamele-Bena, Diane
AU  - Hamele-Bena D
AD  - Department of Clinical Pathology & Cell Biology, Columbia University Medical 
      Center, New York Presbyterian Hospital, New York, New York.
FAU - Wood, Teresa
AU  - Wood T
AD  - Department of Cytology, Columbia University Medical Center, New York Presbyterian 
      Hospital, New York, New York.
FAU - Valladares-Silva, Sunilda
AU  - Valladares-Silva S
AD  - Histology Laboratory, Columbia University Medical Center, New York Presbyterian 
      Hospital, New York, New York.
FAU - Wasserman, Patricia G
AU  - Wasserman PG
AD  - Department of Clinical Pathology & Cell Biology, Columbia University Medical 
      Center, New York Presbyterian Hospital, New York, New York.
LA  - eng
PT  - Journal Article
DEP - 20170706
PL  - United States
TA  - J Am Soc Cytopathol
JT  - Journal of the American Society of Cytopathology
JID - 101613234
OTO - NOTNLM
OT  - Bronchioalveolar lavage
OT  - Cell block
OT  - GMS
OT  - Immunostain
OT  - Pneumocystis jirovecii
EDAT- 2017/01/01 00:00
MHDA- 2017/01/01 00:01
CRDT- 2019/05/03 06:00
PHST- 2017/05/11 00:00 [received]
PHST- 2017/06/30 00:00 [accepted]
PHST- 2019/05/03 06:00 [entrez]
PHST- 2017/01/01 00:00 [pubmed]
PHST- 2017/01/01 00:01 [medline]
AID - S2213-2945(17)30072-8 [pii]
AID - 10.1016/j.jasc.2017.06.207 [doi]
PST - ppublish
SO  - J Am Soc Cytopathol. 2017 Nov-Dec;6(6):242-247. doi: 10.1016/j.jasc.2017.06.207. 
      Epub 2017 Jul 6.