PMID- 31046751
OWN - NLM
STAT- MEDLINE
DCOM- 20191224
LR  - 20200309
IS  - 1472-6831 (Electronic)
IS  - 1472-6831 (Linking)
VI  - 19
IP  - 1
DP  - 2019 May 2
TI  - Influence of epigallocatechin-3-gallate in promoting proliferation and osteogenic 
      differentiation of human periodontal ligament cells.
PG  - 73
LID - 10.1186/s12903-019-0768-7 [doi]
LID - 73
AB  - BACKGROUND: Epigallocatechin-3-gallate (EGCG) was recently proposed to have the 
      potential to regulate bone metabolism, however, its influence on osteogenesis 
      remains controversial. The present study aimed to investigate the effects of EGCG 
      on the proliferation and osteogenesis of human periodontal ligament cells 
      (hPDLCs). METHODS: Cells were cultured in osteogenic medium and treated with EGCG 
      at various concentrations. Cell proliferation was analyzed using a CCK-8 assay 
      and acridine orange (AO)/ethidium bromide (EB) staining. Flow cytometry was used 
      to measure the intracellular reactive oxygen species (ROS) potential of hPDLCs. 
      The expression levels of osteogenic marker genes and proteins in hPDLCs, 
      including type I collagen (COL1), runt-related transcription factor 2 (RUNX2), 
      osteopontin (OPN), and osterix (OSX), were determined by quantitative real-time 
      polymerase chain reaction (qRT-PCR) and western blot analysis. In addition, 
      alkaline phosphatase (ALP) activity was monitored both quantitatively and 
      qualitatively. Extracellular matrix mineralization was further analyzed by 
      alizarin red S staining. RESULTS: The results showed that EGCG concentrations 
      from 6 to 10 muM increased the ROS level and inhibited the cell proliferation of 
      hPDLCs. EGCG concentrations from 2 to 8 muM effectively increased extracellular 
      matrix mineralization, in which 4 and 6 muM EGCG generated the most mineralizing 
      nodules. The ALP activity and the mRNA and protein expression levels of the 
      tested osteogenic markers were most strongly up-regulated by treatment with 4 and 
      6 muM EGCG. CONCLUSIONS: The present study demonstrated that EGCG might promote 
      the osteogenesis of hPDLCs in a dose-dependent manner, with concentrations of 4 
      and 6 muM EGCG showing the strongest osteogenic enhancement without cytotoxicity, 
      indicating a promising role for EGCG in periodontal regeneration in patients with 
      deficient alveolar bone in the future.
FAU - Liu, Jie
AU  - Liu J
AD  - Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, 
      College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
AD  - Department of Prosthodontics, College of Stomatology, Xi'an Jiaotong University, 
      98 Xiwu Road, Xi'an, 710004, Shaanxi, China.
FAU - Lu, Yi
AU  - Lu Y
AD  - Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, 
      College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
FAU - Liu, Jin
AU  - Liu J
AD  - Department of Periodontics, College of Stomatology, Xi'an Jiaotong University, 
      Xi'an, China.
FAU - Jin, Changxiong
AU  - Jin C
AD  - Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, 
      College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
FAU - Meng, Yuchen
AU  - Meng Y
AD  - Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, 
      College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
FAU - Pei, Dandan
AU  - Pei D
AUID- ORCID: 0000-0003-4470-5400
AD  - Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, 
      College of Stomatology, Xi'an Jiaotong University, Xi'an, China. 
      peidandan1986@126.com.
AD  - Department of Prosthodontics, College of Stomatology, Xi'an Jiaotong University, 
      98 Xiwu Road, Xi'an, 710004, Shaanxi, China. peidandan1986@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190502
PL  - England
TA  - BMC Oral Health
JT  - BMC oral health
JID - 101088684
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase
MH  - Catechin/*analogs & derivatives
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Humans
MH  - *Osteogenesis
MH  - *Periodontal Ligament
PMC - PMC6498622
OTO - NOTNLM
OT  - Epigallocatechin-3-gallate
OT  - Human periodontal ligament cells
OT  - Osteogenic differentiation
OT  - Proliferation
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The present study was approved by the 
      Ethics Committee of College of Medicine & Hospital of Stomatology, Xi'an Jiaotong 
      University. All patients or their parents have signed the informed consent form. 
      CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare 
      that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains 
      neutral with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2019/05/03 06:00
MHDA- 2019/12/25 06:00
PMCR- 2019/05/02
CRDT- 2019/05/04 06:00
PHST- 2018/11/05 00:00 [received]
PHST- 2019/04/16 00:00 [accepted]
PHST- 2019/05/04 06:00 [entrez]
PHST- 2019/05/03 06:00 [pubmed]
PHST- 2019/12/25 06:00 [medline]
PHST- 2019/05/02 00:00 [pmc-release]
AID - 10.1186/s12903-019-0768-7 [pii]
AID - 768 [pii]
AID - 10.1186/s12903-019-0768-7 [doi]
PST - epublish
SO  - BMC Oral Health. 2019 May 2;19(1):73. doi: 10.1186/s12903-019-0768-7.