PMID- 31049543
OWN - NLM
STAT- MEDLINE
DCOM- 20191111
LR  - 20240109
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 10
IP  - 5
DP  - 2019 May 22
TI  - Structure and in vitro hypoglycemic activity of a homogenous polysaccharide 
      purified from Sargassum pallidum.
PG  - 2828-2838
LID - 10.1039/c8fo02525h [doi]
AB  - This study aimed at investigating the structure, hypoglycemic activity and the 
      underlying mechanism of a homogeneous polysaccharide (PSP-2) purified from 
      Sargassum pallidum. Structural characterization revealed that PSP-2 with a 
      molecular weight of 144.8 kDa was composed of fucose (21.6%), arabinose (2.5%), 
      galactose (22.4%), glucose (2.2%), xylose (18.8%), mannose (1.2%), glucuronic 
      acid (7.7%) and galacturonic acid (23.6%). The backbone chain of PSP-2 was 
      composed of -->1)-beta-d-Xylp-(3-->, -->1,3)-beta-l-Fucp-(4-->, -->1)-alpha-d-Galp-(6-->, and 
      -->1)-alpha-d-GlcpNAc-(2-->, and the side chains were composed of -->1,3,6)-alpha-d-Galp-(2-->, 
      -->3)-beta-l-Fucp-(1,4-->, beta-d-GalpNAc-(1-->, and alpha-d-Manp-(1-->. In vitro hypoglycemic 
      assays indicated that PSP-2 could significantly enhance glucose consumption, 
      glycogen synthesis, and pyruvate kinase (PK) and hexokinase (HK) activities of 
      insulin-resistant HepG2 cells. Furthermore, the underlying mechanistic studies 
      revealed that PSP-2 could ameliorate insulin resistance by up-regulating the 
      expression levels of insulin receptor substrate-1 (IRS-1), glycogen synthase 
      (GS), phosphoinositide-3-kinase (PI3K) and glucose transporter-4 (GLUT4). These 
      results suggested that PSP-2 may be a potential candidate for the prevention and 
      treatment of Type 2 diabetes mellitus.
FAU - Cao, Changliang
AU  - Cao C
AD  - School of Food Science and Engineering, South China University of Technology, 
      Guangzhou 510640, China. felichao@scut.edu.cn lfxfu@scut.edu.cn.
FAU - Zhang, Bin
AU  - Zhang B
FAU - Li, Chao
AU  - Li C
FAU - Huang, Qiang
AU  - Huang Q
FAU - Fu, Xiong
AU  - Fu X
FAU - Liu, Rui Hai
AU  - Liu RH
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
RN  - EC 2.4.1.11 (Glycogen Synthase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Gene Expression/drug effects
MH  - Glucose/metabolism
MH  - Glucose Transporter Type 4/genetics/metabolism
MH  - Glycogen Synthase/genetics/metabolism
MH  - Hep G2 Cells
MH  - Humans
MH  - Hypoglycemic Agents/*chemistry/pharmacology
MH  - Insulin Receptor Substrate Proteins/genetics/metabolism
MH  - Molecular Weight
MH  - Plant Extracts/*chemistry/pharmacology
MH  - Polysaccharides/*chemistry/pharmacology
MH  - Sargassum/*chemistry
EDAT- 2019/05/03 06:00
MHDA- 2019/11/12 06:00
CRDT- 2019/05/04 06:00
PHST- 2019/05/03 06:00 [pubmed]
PHST- 2019/11/12 06:00 [medline]
PHST- 2019/05/04 06:00 [entrez]
AID - 10.1039/c8fo02525h [doi]
PST - ppublish
SO  - Food Funct. 2019 May 22;10(5):2828-2838. doi: 10.1039/c8fo02525h.