PMID- 31050631
OWN - NLM
STAT- MEDLINE
DCOM- 20200218
LR  - 20200218
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 100
IP  - 6
DP  - 2019 Jun
TI  - Diverse novel phleboviruses in sandflies from the Panama Canal area, Central 
      Panama.
PG  - 938-949
LID - 10.1099/jgv.0.001260 [doi]
AB  - The genus Phlebovirus (order Bunyavirales, family Phenuiviridae) comprises 57 
      viruses that are grouped into nine species-complexes. Sandfly-transmitted 
      phleboviruses are found in Europe, Africa and the Americas and are responsible 
      for febrile illness and infections of the nervous system in humans. The aim of 
      this study was to assess the genetic diversity of sandfly-transmitted 
      phleboviruses in connected and isolated forest habitats throughout the Panama 
      Canal area in Central Panama. In total, we collected 13 807 sandflies comprising 
      eight phlebotomine species. We detected several strains pertaining to five 
      previously unknown viruses showing maximum pairwise identities of 45-78 % to the 
      RNA-dependent RNA polymerase genes of phleboviruses. Entire coding regions were 
      directly sequenced from infected sandflies as virus isolation in cell culture was 
      not successful. The viruses were tentatively named La Gloria virus (LAGV), Mona 
      Grita virus (MOGV), Pena Blanca virus (PEBV), Tico virus (TICV) and Tres 
      Almendras virus (TRAV). Inferred phylogenies and p-distance-based analyses 
      revealed that PEBV groups with the Bujaru phlebovirus species-complex, TRAV with 
      the Candiru phlebovirus species-complex and MOGV belongs to the proposed Icoarci 
      phlebovirus species-complex, whereas LAGV and TICV seem to be distant members of 
      the Bujaru phlebovirus species-complex. No specific vector or habitat association 
      was found for any of the five viruses. Relative abundance of sandflies was 
      similar over habitat types. Our study shows that blood-feeding insects 
      originating from remote and biodiverse habitats harbour multiple previously 
      unknown phleboviruses. These viruses should be included in future surveillance 
      studies to assess their geographic distribution and to elucidate if these viruses 
      cause symptoms of disease in animals or humans.
FAU - Marklewitz, Marco
AU  - Marklewitz M
AD  - 3 Smithsonian Tropical Research Institute, Panama City, Republic of Panama.
AD  - 1 Institute of Virology, Charite - Universitatsmedizin Berlin, corporate member 
      of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of 
      Health, Berlin, Germany.
AD  - 2 German Center for Infection Research (DZIF), Berlin, Germany.
FAU - Dutari, Larissa C
AU  - Dutari LC
AD  - 4 Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de 
      Investigaciones Cientificas y Servicios de Alta Tecnologia (INDICASAT-AIP), 
      Panama City, Republic of Panama.
AD  - 5 Biotechnology Department, Acharya Nagarjuna University, Guntur, India.
AD  - 6 Programa Centroamericano de Maestria en Entomologia, Vicerrectoria de 
      Investigacion y, Postgrado, Universidad de Panama, Panama City, Republic of 
      Panama.
FAU - Paraskevopoulou, Sofia
AU  - Paraskevopoulou S
AD  - 1 Institute of Virology, Charite - Universitatsmedizin Berlin, corporate member 
      of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of 
      Health, Berlin, Germany.
FAU - Page, Rachel A
AU  - Page RA
AD  - 3 Smithsonian Tropical Research Institute, Panama City, Republic of Panama.
FAU - Loaiza, Jose R
AU  - Loaiza JR
AD  - 3 Smithsonian Tropical Research Institute, Panama City, Republic of Panama.
AD  - 6 Programa Centroamericano de Maestria en Entomologia, Vicerrectoria de 
      Investigacion y, Postgrado, Universidad de Panama, Panama City, Republic of 
      Panama.
AD  - 4 Centro de Biodiversidad y Descubrimiento de Drogas, Instituto de 
      Investigaciones Cientificas y Servicios de Alta Tecnologia (INDICASAT-AIP), 
      Panama City, Republic of Panama.
FAU - Junglen, Sandra
AU  - Junglen S
AD  - 1 Institute of Virology, Charite - Universitatsmedizin Berlin, corporate member 
      of Free University Berlin, Humboldt-University Berlin, and Berlin Institute of 
      Health, Berlin, Germany.
AD  - 2 German Center for Infection Research (DZIF), Berlin, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190503
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
SB  - IM
MH  - Africa
MH  - Animals
MH  - Europe
MH  - Genome, Viral/genetics
MH  - Humans
MH  - Insect Vectors/virology
MH  - Panama
MH  - Phlebotomus Fever/virology
MH  - Phlebovirus/*genetics/*isolation & purification
MH  - Phylogeny
MH  - Psychodidae/*virology
OTO - NOTNLM
OT  - Bunyavirales
OT  - Neotropics
OT  - Panama
OT  - Phlebotominae
OT  - Phlebovirus
OT  - species-complex
EDAT- 2019/05/06 06:00
MHDA- 2020/02/19 06:00
CRDT- 2019/05/04 06:00
PHST- 2019/05/06 06:00 [pubmed]
PHST- 2020/02/19 06:00 [medline]
PHST- 2019/05/04 06:00 [entrez]
AID - 10.1099/jgv.0.001260 [doi]
PST - ppublish
SO  - J Gen Virol. 2019 Jun;100(6):938-949. doi: 10.1099/jgv.0.001260. Epub 2019 May 3.