PMID- 31052759 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20191120 IS - 1094-4087 (Electronic) IS - 1094-4087 (Linking) VI - 27 IP - 9 DP - 2019 Apr 29 TI - Two-photon Bessel beam tomography for fast volume imaging. PG - 12147-12162 LID - 10.1364/OE.27.012147 [doi] AB - Light microscopy on dynamic samples, for example neural activity in the brain, often requires imaging volumes that extend over several 100 microm in axial direction at a rate of at least several tens of Hertz. Here, we develop a tomography approach for scanning fluorescence microscopy which allows recording a volume image in a single frame scan. Volumes are imaged by simultaneously recording four independent projections at different angles using temporally multiplexed, tilted Bessel beams. From the resulting projections, three-dimensional images are reconstructed using inverse Radon transforms combined with convolutional neural networks (U-net). FAU - Valle, Andres Flores AU - Valle AF FAU - Seelig, Johannes D AU - Seelig JD LA - eng PT - Journal Article PL - United States TA - Opt Express JT - Optics express JID - 101137103 EDAT- 2019/05/06 06:00 MHDA- 2019/05/06 06:01 CRDT- 2019/05/05 06:00 PHST- 2019/05/05 06:00 [entrez] PHST- 2019/05/06 06:00 [pubmed] PHST- 2019/05/06 06:01 [medline] AID - 409061 [pii] AID - 10.1364/OE.27.012147 [doi] PST - ppublish SO - Opt Express. 2019 Apr 29;27(9):12147-12162. doi: 10.1364/OE.27.012147.