PMID- 31053587
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20221207
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 85
IP  - 14
DP  - 2019 Jul 15
TI  - Stable-Isotope Probing-Enabled Cultivation of the Indigenous Bacterium Ralstonia 
      sp. Strain M1, Capable of Degrading Phenanthrene and Biphenyl in Industrial 
      Wastewater.
LID - 10.1128/AEM.00511-19 [doi]
LID - e00511-19
AB  - To identify and obtain the indigenous degraders metabolizing phenanthrene (PHE) 
      and biphenyl (BP) from the complex microbial community within industrial 
      wastewater, DNA-based stable-isotope probing (DNA-SIP) and cultivation-based 
      methods were applied in the present study. DNA-SIP results showed that two 
      bacterial taxa (Vogesella and Alicyclobacillus) were considered the key 
      biodegraders responsible for PHE biodegradation only, whereas Bacillus and 
      Cupriavidus were involved in BP degradation. Vogesella and Alicyclobacillus have 
      not been linked with PHE degradation previously. Additionally, DNA-SIP helped 
      reveal the taxonomic identity of Ralstonia-like degraders involved in both PHE 
      and BP degradation. To target the separation of functional Ralstonia-like 
      degraders from the wastewater, we modified the traditional cultivation medium and 
      culture conditions. Finally, an indigenous PHE- and BP-degrading strain, 
      Ralstonia pickettii M1, was isolated via a cultivation-dependent method, and its 
      role in PHE and BP degradation was confirmed by enrichment of the 16S rRNA gene 
      and distinctive dioxygenase genes in the DNA-SIP experiment. Our study has 
      successfully established a program for the application of DNA-SIP in the 
      isolation of the active functional degraders from an environment. It also deepens 
      our insight into the diversity of indigenous PHE- and BP-degrading 
      communities.IMPORTANCE The comprehensive treatment of wastewater in industrial 
      parks suffers from the presence of multiple persistent organic pollutants (POPs), 
      such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls 
      (PCBs), which reduce the activity of activated sludge and are difficult to 
      eliminate. Characterizing and applying active bacterial degraders metabolizing 
      multiple POPs therefore helps to reveal the mechanisms of synergistic metabolism 
      and to improve wastewater treatment efficiency in industrial parks. To date, SIP 
      studies have successfully investigated the biodegradation of PAHs or PCBs in 
      real-world habitats. DNA-SIP facilitates the isolation of target microorganisms 
      that pose environmental concerns. Here, an indigenous phenanthrene (PHE)- and 
      biphenyl (BP)-degrading strain in wastewater, Ralstonia pickettii M1, was 
      isolated via a cultivation-dependent method, and its role in PHE and BP 
      degradation was confirmed by DNA-SIP. Our study provides a routine protocol for 
      the application of DNA-SIP in the isolation of the active functional degraders 
      from an environment.
CI  - Copyright (c) 2019 American Society for Microbiology.
FAU - Li, Jibing
AU  - Li J
AD  - State Key Laboratory of Organic Geochemistry, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
FAU - Luo, Chunling
AU  - Luo C
AD  - State Key Laboratory of Organic Geochemistry, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou, China clluo@gig.ac.cn.
AD  - College of Natural Resources and Environment, South China Agricultural 
      University, Guangzhou, China.
FAU - Zhang, Dayi
AU  - Zhang D
AD  - School of Environment, Tsinghua University, Beijing, China.
FAU - Cai, Xixi
AU  - Cai X
AD  - College of Resources and Environment, Fujian Agriculture and Forestry University, 
      Fuzhou, China.
FAU - Jiang, Longfei
AU  - Jiang L
AD  - State Key Laboratory of Organic Geochemistry, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
FAU - Zhang, Gan
AU  - Zhang G
AD  - State Key Laboratory of Organic Geochemistry, Guangzhou Institute of 
      Geochemistry, Chinese Academy of Sciences, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190701
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Industrial Waste)
RN  - 0 (Phenanthrenes)
RN  - 0 (Waste Water)
RN  - 0 (Water Pollutants, Chemical)
RN  - 2L9GJK6MGN (diphenyl)
RN  - 448J8E5BST (phenanthrene)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Biphenyl Compounds/*metabolism
MH  - Industrial Waste/analysis
MH  - Phenanthrenes/*metabolism
MH  - Ralstonia/*metabolism
MH  - Species Specificity
MH  - *Waste Disposal, Fluid
MH  - Wastewater/*microbiology
MH  - Water Pollutants, Chemical/*analysis
PMC - PMC6606873
OTO - NOTNLM
OT  - active degraders
OT  - biphenyl
OT  - distinctive dioxygenase genes
OT  - phenanthrene
OT  - stable-isotope probing (SIP)
EDAT- 2019/05/06 06:00
MHDA- 2020/06/23 06:00
PMCR- 2020/01/01
CRDT- 2019/05/05 06:00
PHST- 2019/03/05 00:00 [received]
PHST- 2019/04/26 00:00 [accepted]
PHST- 2019/05/06 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2019/05/05 06:00 [entrez]
PHST- 2020/01/01 00:00 [pmc-release]
AID - AEM.00511-19 [pii]
AID - 00511-19 [pii]
AID - 10.1128/AEM.00511-19 [doi]
PST - epublish
SO  - Appl Environ Microbiol. 2019 Jul 1;85(14):e00511-19. doi: 10.1128/AEM.00511-19. 
      Print 2019 Jul 15.