PMID- 31054452 OWN - NLM STAT- MEDLINE DCOM- 20190617 LR - 20190617 IS - 1873-376X (Electronic) IS - 1570-0232 (Linking) VI - 1118-1119 DP - 2019 Jun 15 TI - Preparation of molecularly imprinted polymers coupled with high-performance liquid chromatography for the selective extraction of salidroside from Rhodiola crenulata. PG - 180-186 LID - S1570-0232(19)30054-6 [pii] LID - 10.1016/j.jchromb.2019.04.004 [doi] AB - Salidroside is one of the bio-active compounds found in Rhodiola crenulata. To find an easy, time saving and efficient way to extract, purify and enrich salidroside from Rhodiola and other natural plants, we prepared a highly selective molecularly imprinted polymer (MIP) for extraction and preconcentration of salidroside using salidroside (SD) as a template, acrylamide (AM) as a functional monomer, ethylene glycol dimethacrylate (EDMA) as a crosslinking monomer, and dimethyl formamide (DMF) as a porogen. The performance of the MIPs was evaluated through selective recognition capacity and adsorption isotherms and kinetics. The results showed that MIPs possessed excellent specific recognition toward SD and could effectively discriminate its structural analogue. The application of the developed MIPs as a selective sorbent for solid-phase extraction (SPE) of SD was also investigated. Under the optimum conditions, a rapid, economical, and efficient method based upon MIP-SPE coupled with high-performance liquid chromatography (HPLC) was developed for the determination of SD in Rhodiola crenulata. The method showed satisfactory recoveries (from spiked real samples at 3 fortification levels of 0.5, 1 and 10 mg L(-1)) of 88.74%- 97.64% with relative standard deviations (RSDs) ranging from 2.05%-3.54%. Furthermore, MIP-SPE was successfully used to separate and purify SD from different parts in Rhodiola crenulata and it should be available for determination of salidroside in others herbs. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Yu, Hailong AU - Yu H AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Agri-food Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; College of Bioengineering, Beijing Polytechnic, Beijing 100176, PR China. FAU - He, Yahui AU - He Y AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Agri-food Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China; Beijing Purkinje General Iinstrument Co., Ltd., Beijing 100081, PR China. FAU - She, Yongxin AU - She Y AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Agri-food Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: sheyongxin@caas.cn. FAU - Wang, Miao AU - Wang M AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Agri-food Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China. FAU - Yan, Zheng AU - Yan Z AD - College of Bioengineering, Beijing Polytechnic, Beijing 100176, PR China. FAU - Ren, Jian Hua AU - Ren JH AD - College of Bioengineering, Beijing Polytechnic, Beijing 100176, PR China. FAU - Cao, Zhen AU - Cao Z AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Agri-food Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China. FAU - Shao, Yong AU - Shao Y AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Agri-food Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China. FAU - Wang, Shanshan AU - Wang S AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240 Erzurum, Turkey. FAU - Hacimuftuoglu, Ahmet AU - Hacimuftuoglu A AD - Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240 Erzurum, Turkey. FAU - Wang, Jing AU - Wang J AD - Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China; Key Laboratory of Agri-food Safety and Quality, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: wangjing05@caas.cn. LA - eng PT - Journal Article DEP - 20190416 PL - Netherlands TA - J Chromatogr B Analyt Technol Biomed Life Sci JT - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JID - 101139554 RN - 0 (Glucosides) RN - 0 (Phenols) RN - 0 (Plant Extracts) RN - M983H6N1S9 (rhodioloside) SB - IM MH - Chromatography, High Pressure Liquid/*methods MH - Glucosides/analysis/chemistry/*isolation & purification MH - Limit of Detection MH - Linear Models MH - Molecular Imprinting/*methods MH - Phenols/analysis/chemistry/*isolation & purification MH - Plant Extracts/chemistry MH - Reproducibility of Results MH - Rhodiola/*chemistry MH - Solid Phase Extraction/*methods OTO - NOTNLM OT - Active compound OT - HPLC OT - MIP OT - Rhodiola crenulata OT - Salidroside EDAT- 2019/05/06 06:00 MHDA- 2019/06/18 06:00 CRDT- 2019/05/05 06:00 PHST- 2019/01/09 00:00 [received] PHST- 2019/03/31 00:00 [revised] PHST- 2019/04/02 00:00 [accepted] PHST- 2019/05/06 06:00 [pubmed] PHST- 2019/06/18 06:00 [medline] PHST- 2019/05/05 06:00 [entrez] AID - S1570-0232(19)30054-6 [pii] AID - 10.1016/j.jchromb.2019.04.004 [doi] PST - ppublish SO - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jun 15;1118-1119:180-186. doi: 10.1016/j.jchromb.2019.04.004. Epub 2019 Apr 16.